Ryanodine Receptor antibody | RYR.1 (G-1)

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Mouse anti Human Ryanodine Receptor

Product Type
Monoclonal Antibody
Clone
RYR.1 (G-1)
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT1818 C E F IB IF* 50 µg
Mouse anti Human Ryanodine Receptor antibody, clone RYR-1 recognizes the human ryanodine receptor 1, also known as Skeletal muscle calcium release channel or skeletal muscle ryanodine receptor. Ryanodine receptor 1 is a is a large 5038 amino acid ~500 kDa multi pass sarcoplamic reticulum associated transmembrane protein containing three B30.2/SPRY domains and five MIR domains. Mouse anti Human Ryanodine Receptor antibody, clone RYR-1 recognizes an epitope within the C-terminal cytoplasmic domain of the ryanodine receptor (UniProt: P21817).

Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscle cells. Mouse anti Human Ryanodine Receptor antibody, clone RYR-1 reacts with ryanodine receptor isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues). Defects in the ryanodine receptor 1 gene are associated with malignant hyperthermia (Gillard et al. 1992), central core disease of muscle (Quane et al. 1993) and certain congential myopathies (Monnier et al. 2000).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Dog
Bovine
Pig
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Sodium azide
Immunogen
Short synthetic polypeptide corresponding to the C-terminal domain of the ryanodine receptor.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P21817 Related reagents
Entrez Gene
RYR1 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0001666 response to hypoxia
GO:0006936 muscle contraction
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005219 ryanodine-sensitive calcium-release channel activity
GO:0005516 calmodulin binding
GO:0005790 smooth endoplasmic reticulum
GO:0005938 cell cortex
GO:0014701 junctional sarcoplasmic reticulum membrane
GO:0014802 terminal cisterna
GO:0031000 response to caffeine
GO:0031674 I band
GO:0051209 release of sequestered calcium ion into cytosol
Regulatory
For research purposes only

Applications of Ryanodine Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000
Flow Cytometry 1/500
Immunoblotting 1/100
Immunofluorescence 1 1/100
Immunohistology - Frozen 1/100
  1. 1 Use indirect immunofluorescence. Visualization by confocal microscopy is required, as detection by standard fluorescent microscopy will not be adequate to detect the RyR. Additionally, fluorescent, not enzymatic, detection is required. Due to the intensity of confocal lasers, use of an anti-fading agent, such as DABCO, is strongly recommended.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2b Negative Control MCA691 100 Tests F