Protein Gene Product 9.5 antibody | BH7

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Anti Human Protein Gene Product 9.5 Antibody, clone BH7 thumbnail image 1

Anti Human Protein Gene Product 9.5 Antibody, clone BH7 thumbnail image 2

Anti Human Protein Gene Product 9.5 Antibody, clone BH7 gallery image 1 — Immunostaining of rat spinal cord section with anti-mouse PGP9.5 (red) and anti-neurofiliament H (green) antibodies

Anti Human Protein Gene Product 9.5 Antibody, clone BH7 gallery image 2 — Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa PGP9.5 (UCH-L1) protein

Mouse anti Human Protein Gene Product 9.5

Product Type: Monoclonal Antibody
Clone: BH7
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA4750GA	IF WB			0.1 ml

Mouse anti Human protein gene product 9.5 monoclonal antibody, clone BH7 recognizes protein gene product 9.5 (PGP9.5) also known as ubiquitin C terminal hydrolase 1 (UCH-L1). UCH-L1 is a thiol protease that recognises and hydrolyses a peptide bond at the C-terminal glycine of ubiquitin. This protein is closely related to UCH-L3 and both are thought to recycle ubiquitin to maintain pools of monomeric ubiquitin necessary for proteolysis. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and their tumours.

UCH-L1 and UCH-L3 have been shown to have separate and overlapping functions in the maintanence of neurons of the gracile tract, nucleus tractus solitarius (NTS) and area postrema (Kurihara et al. 2001) .

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mammals	Expected from Sequence
Mouse
Rat
Bovine

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.065% Sodium Azide (NaN₃)

Immunogen

Recombinant full length human PGP9.5 purified from E.coli.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch

More Information

UniProt: P09936 Related reagents; Q9R0P9 Related reagents; Q00981 Related reagents; P23356 Related reagents
Entrez Gene: UCHL1 Related reagents; UCHL1 Related reagents; Uchl1 Related reagents; Uchl1 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0004197 cysteine-type endopeptidase activity; GO:0004221 ubiquitin thiolesterase activity; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0006511 ubiquitin-dependent protein catabolic process; GO:0008242 omega peptidase activity; GO:0016579 protein deubiquitination; GO:0016874 ligase activity; GO:0031694 alpha-2A adrenergic receptor binding; GO:0043130 ubiquitin binding; GO:0043407 negative regulation of MAP kinase activity; GO:0008234 cysteine-type peptidase activity; GO:0019233 sensory perception of pain; GO:0005829 cytosol; GO:0006950 response to stress; GO:0007412 axon target recognition; GO:0007628 adult walking behavior; GO:0008283 cell proliferation; GO:0019896 axon transport of mitochondrion; GO:0030424 axon; GO:0042755 eating behavior; GO:0043025 neuronal cell body; GO:0050905 neuromuscular process
Regulatory: For research purposes only

Applications of Protein Gene Product 9.5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence			1/500
Western Blotting			1/5000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Clone BH7 detects a band of approximately 24 kDa in rat hippocampal lysate

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Immunofluorescence

Product Specific References

References for Protein Gene Product 9.5 antibody

Peyrot des Gachons, C. et al. (2011) Unusual pungency from extra-virgin olive oil is attributable to restricted spatial expression of the receptor of oleocanthal.
J Neurosci. 31: 999-1009.

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