Protein Gene Product 9.5 antibody | BH7
Mouse anti Human Protein Gene Product 9.5 antibody, clone BH7 (MCA4750GA) used to identify PGP9.5 expressing spermatogonia in seminiferous tubules of rhesus macaques by immunofluorescence.
Image caption:
Germ cell characterization in testes from monkeys at different ages. (A) Seminiferous tubules are shown in neonatal (6 M), juvenile (18 M), and adult (60 M) rhesus monkey testes. The histologic morphology of seminiferous tubules was demonstrated by H&E staining. (B) Germ cells in the testes were identified by UCHL1 marker as spermatogonia (B, green). DAPI was applied as a nuclear stain (scale bars, 50μm)
From: Yuan H, Sun J, Wang S, Xiang Z, Yang F, Yan Y, Duan Y, Li L, Wu X, Si W.
Primary culture of germ cells that portray stem cell characteristics and recipient preparation for autologous transplantation in the rhesus monkey.
J Cell Mol Med. 2022 Mar;26(5):1567-1578.
doi: 10.1111/jcmm.17197.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
IF WB ResetMouse anti Human Protein Gene Product 9.5
- Product Type
- Monoclonal Antibody
- Clone
- BH7
- Isotype
- IgG1
- Specificity
- Protein Gene Product 9.5
Antibody Quality and 
Unrivaled 
| Mouse anti Human protein gene product 9.5 monoclonal antibody, clone BH7 recognizes protein gene product 9.5 (PGP9.5) also known as ubiquitin C terminal hydrolase 1 (UCH-L1). UCH-L1 is a thiol protease that recognises and hydrolyses a peptide bond at the C-terminal glycine of ubiquitin. This protein is closely related to UCH-L3 and both are thought to recycle ubiquitin to maintain pools of monomeric ubiquitin necessary for proteolysis. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and their tumours. UCH-L1 and UCH-L3 have been shown to have separate and overlapping functions in the maintanence of neurons of the gracile tract, nucleus tractus solitarius (NTS) and area postrema (Kurihara et al. 2001) . |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mammals Expected from Sequence Mouse Rat Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.03% Sodium Azide 50% Glycerol - Immunogen
- Recombinant full length human PGP9.5 purified from E.coli.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
1/500 | |
| Western Blotting | |
1/5000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Clone BH7 detects a band of approximately 24 kDa in rat hippocampal lysate
References for Protein Gene Product 9.5 antibody
-
Peyrot des Gachons, C. et al. (2011) Unusual pungency from extra-virgin olive oil is attributable to restricted spatial expression of the receptor of oleocanthal.
J Neurosci. 31: 999-1009. -
Yuan, H. et al. (2022) Primary culture of germ cells that portray stem cell characteristics and recipient preparation for autologous transplantation in the rhesus monkey.
J Cell Mol Med. 26 (5): 1567-78.
- Synonyms
- PGP 9.5
- UCHL1
- UniProt
- P09936
- Q9R0P9
- Q00981
- P23356
- Entrez Gene
- UCHL1
- UCHL1
- Uchl1
- Uchl1
- GO Terms
- GO:0005886 plasma membrane
- GO:0004197 cysteine-type endopeptidase activity
- GO:0004221 ubiquitin thiolesterase activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006511 ubiquitin-dependent protein catabolic process
- GO:0008242 omega peptidase activity
- GO:0016579 protein deubiquitination
- GO:0016874 ligase activity
- View More GO Terms
- GO:0031694 alpha-2A adrenergic receptor binding
- GO:0043130 ubiquitin binding
- GO:0043407 negative regulation of MAP kinase activity
- GO:0008234 cysteine-type peptidase activity
- GO:0019233 sensory perception of pain
- GO:0005829 cytosol
- GO:0006950 response to stress
- GO:0007412 axon target recognition
- GO:0007628 adult walking behavior
- GO:0008283 cell proliferation
- GO:0019896 axon transport of mitochondrion
- GO:0030424 axon
- GO:0042755 eating behavior
- GO:0043025 neuronal cell body
- GO:0050905 neuromuscular process
View more products with PROTEIN GENE PRODUCT 9.5 specificity
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