Protein Gene Product 9.5 antibody | 31A3

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Mouse anti Human Protein Gene Product 9.5

Product Type
Monoclonal Antibody
Clone
31A3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
7863-1004 E P* WB 0.2 mg
Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubuiquitin pathway.

Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumours of the DNES.

Clones 31A3 and 13C4 each recognise a different epitope towards the N-terminus of the protein. Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 also recognizes PGP9.5 in other species, including rat and rabbit but evidence suggests it does not bind to PGP9.5 in guinea pigs (Wilson et al. 1988). Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 has been used successfully as a capture reagent with clone 13C4 as a detection reagent in a sandwich ELISA to evaluate contamination of processed meat samples with neuronal tissue (Gaunitz et al. 2009).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Rabbit
Guinea Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Native, from brain
Approx. Protein Concentrations
1mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P09936 Related reagents
Entrez Gene
UCHL1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0004197 cysteine-type endopeptidase activity
GO:0004221 ubiquitin thiolesterase activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0008242 omega peptidase activity
GO:0016579 protein deubiquitination
GO:0016874 ligase activity
GO:0031694 alpha-2A adrenergic receptor binding
GO:0043130 ubiquitin binding
GO:0043407 negative regulation of MAP kinase activity
Regulatory
For research purposes only

Applications of Protein Gene Product 9.5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/2000
Immunohistology - Paraffin 1 1/100 1/400
Western Blotting
  1. 1 This antibody is suitable for use on paraffin embedded tissue sections. We do however recommend fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding. Specimens which have not been fixed in acetic acid/alcohol will require pretreatment using the microwave-citrate buffer method.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Product Specific References

Source Reference

  1. Day, I.N.M and Thompson, R.J. (1986) Monoclonal antibodies to the neuroendocrine protein PGP 9.5
    Biochemical Society Transactions 14: 350–1.

References for Protein Gene Product 9.5 antibody

  1. Wilson, P.O. et al. (1988) The immunolocalization of protein gene product 9.5 using rabbit polyclonal and mouse monoclonal antibodies.
    Br J Exp Pathol. 69 (1): 91-104.
  2. Nonclercq, D. et al. (2002) Phenotypic variations and dynamic topography of transformed cells in an experimental model of diethylstilbestrol-induced renal tumour in male Syrian hamster.
    Histochem J. 34 (10): 487-97.
  3. García-Añoveros, J. et al. (2001) Transport and localization of the DEG/ENaC ion channel BNaC1alpha to peripheral mechanosensory terminals of dorsal root ganglia neurons.
    J Neurosci. 21 (8): 2678-86.
  4. Verhein, K.C. et al. (2011) Three days after a single exposure to ozone, the mechanism of airway hyperreactivity is dependent on substance P and nerve growth factor.
    Am J Physiol Lung Cell Mol Physiol. 300: L176-84.
  5. Caballero, O.L. et al. (2002) Interaction and colocalization of PGP9.5 withJAB1 and p27(Kip1).
    Oncogene. 21:3003-10.
  6. Kondo, T. et al. (2013) Prostaglandin E2 mediates acid-induced heartburn in healthy volunteers.
    Am J Physiol Gastrointest Liver Physiol. 304(6):G568-73
  7. Mihara, H. et al. (2010) Involvement of TRPV2 activation in intestinal movement through nitric oxide production in mice.
    J Neurosci. 30: 16536-44.
  8. Forsgren, K. et al. (1999) Regeneration of nerve fibres in the maxillary sinus mucosa after experimental surgery. An immunocytochemical double-labelling study in the rabbit.
    Acta Otolaryngol. 119: 486-91.
  9. Yee, C.L. et al. (2001) "Type III" cells of rat taste buds: immunohistochemical and ultrastructural studies of neuron-specific enolase, protein gene product 9.5, and serotonin.
    J Comp Neurol. 440: 97-108.
  10. Pauza, D.H. et al. (2014) A combined acetylcholinesterase and immunohistochemical method for precise anatomical analysis of intrinsic cardiac neural structures.
    Ann Anat. pii: S0940-9602(14)00159-9.
  11. Sugimoto, K. et al. (2011) Olmesartan ameliorates peripheral nerve dysfunction in Zucker diabetic fatty rats.
    J Hypertens. 29: 1337-46.
  12. Bishop, P. et al. (2014) The ubiquitin C-terminal hydrolase L1 (UCH-L1) C terminus plays a key role in protein stability, but its farnesylation is not required for membrane association in primary neurons.
    J Biol Chem. 289 (52): 36140-9.
  13. Moldovan, M. et al. (2013) Peptide mimetic of the S100A4 protein modulates peripheral nerve regeneration and attenuates the progression of neuropathy in myelin protein P0 null mice.
    Mol Med. 19: 43-53.
  14. Kaleczyc, J. et al. (2007) The distribution and chemical coding of intramural neurons supplying the porcine stomach - the study on normal pigs and on animals suffering from swine dysentery.
    Anat Histol Embryol. 36 (3): 186-93.
  15. Silva, I. et al. (2015) Activation of P2Y6 Receptors Facilitates Nonneuronal Adenosine Triphosphate and Acetylcholine Release from Urothelium with the Lamina Propria of Men with Bladder Outlet Obstruction.
    J Urol. 194 (4): 1146-54.
  16. Gaunitz C et al. (2009) Suitability of antigens PGP 9.5 and neurofilament light as marker proteins for detection of neuronal tissue in processed meat products.
    J Food Prot. 72 (5): 1070-7.
  17. Forsgren, K. et al. (1999) Regeneration of nerve fibres in the maxillary sinus mucosa after experimental surgery. An immunocytochemical double-labelling study in the rabbit.
    Acta Otolaryngol. 119 (4): 486-91.
  18. Pauziene, N. et al. (2016) Innervation of the rabbit cardiac ventricles.
    J Anat. 228 (1): 26-46.
  19. Rashwan, A. et al. (2016) Ontogeny and innervation of taste buds in mouse palatal gustatory epithelium.
    J Chem Neuroanat. 71: 26-40.
  20. Inokaitis, H. et al. (2016) Innervation of sinoatrial nodal cells in the rabbit.
    Ann Anat. Apr 1. pii: S0940-9602(16)30039-5. [Epub ahead of print]
  21. Lee, W-Y. et al. (2016) Establishment of a surgically induced cryptorchidism canine recipient model for spermatogonial stem cell transplantation.
    Laboratory Animal Research. 32 (4): 257.
  22. Lee, K.H. et al. (2016) Vitrified canine testicular cells allow the formation of spermatogonial stem cells and seminiferous tubules following their xenotransplantation into nude mice.
    Sci Rep. 6: 21919.
  23. Lakritz, J.R. et al. (2017) An oral form of methylglyoxal-bis-guanylhydrazone reduces monocyte activation and traffic to the dorsal root ganglia in a primate model of HIV-peripheral neuropathy.
    J Neurovirol. May 1 [Epub ahead of print]
  24. Day, I.N. & Thompson, R.J. (2010) UCHL1 (PGP 9.5): neuronal biomarker and ubiquitin system protein.
    Prog Neurobiol. 90 (3): 327-62.
  25. Park, H.J. et al. (2017) Stage-specific expression of Sal-like protein 4 in boar testicular germ cells.
    Theriogenology. 101: 44-52.
  26. Hur, T.Y. et al. (2017) Dose-dependent effects of busulfan on dog testes in preparation for spermatogonial stem cell transplantation.
    Lab Anim Res. 33 (3): 264-9.
  27. Park, H.J. et al. (2018) Species-specific expression of phosphoglycerate kinase 2 (PGK2) in the developing porcine testis.
    Theriogenology. 110: 158-167.
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