POLR3A antibody | AB01/1E10

Mouse anti Human POLR3A

Product Type
Monoclonal Antibody
Clone
AB01/1E10
Isotype
IgG1
Format
Purified
Specificity
POLR3A

Product Code Applications Pack Size List Price Your Price Qty
New MCA6411GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 0.1 mg loader
List Price Your Price
loader

Mouse anti Human POLR3A antibody recognizes POLR3A, otherwise known as DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase III subunit A, RNA polymerase III 155kDa subunit and RNA polymerase III subunit C160.

DNA-directed RNA polymerase is responsible for catalyzing the transcription of DNA into RNA via the four ribonucleoside triphosphates as substrates. It constitutes the largest and catalytic core of RNA polymerase III and transcribes small RNAs such as 5S rRNA and tRNAs; molecules essential for the normal functioning and survival of cells (Dieci et al. 2007). This protein plays a critical role in detecting and controlling infection by intracellular bacteria and DNA viruses. It also acts as a non-self double stranded DNA sensor in the innate immune response. Synthesis of non–self DNA into RNA leads to type I interferon production via the RIG-1 pathway (Chiu et al. 2009). Mutations in the POLR3A gene have been linked to a cluster of inherited neurodegenerative disorders called Hypomyelinating Leukodystrophy (Bernard et al. 2011). Furthermore, studies show the possible connection of the mutated POLR3A gene to autoimmune diseases such as Scleroerma.

Mouse anti Human POLR3A antibody detects a band of 157 kDa.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti Human POLR3A antibody detects a band of 157 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant protein, aa 600-996 of human POLR3A
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
O14802
Entrez Gene
POLR3A
GO Terms
GO:0003677 DNA binding
GO:0009615 response to virus
GO:0003899 DNA-directed RNA polymerase activity
GO:0005666 DNA-directed RNA polymerase III complex
GO:0006385 transcription elongation from RNA polymerase III promoter
GO:0006386 termination of RNA polymerase III transcription
GO:0008270 zinc ion binding
GO:0032549 ribonucleoside binding
GO:0032728 positive regulation of interferon-beta production
GO:0045087 innate immune response

MCA6411GA

100007433

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