p53 antibody | DO-1



Mouse anti Human p53 antibody, clone DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25,common to isoforms 1-3 of p53.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Bovine Cat Horse Green Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant human p53.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
Applications of p53 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Paraffin | Neat | ||
ELISA | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 | 1/25 | ||
Immunoprecipitation | |||
Western Blotting | 1/1000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Colon or breast carcinoma
Secondary Antibodies Available
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for p53 antibody
-
Vojtěsek B et al. (1992) An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53.
J Immunol Methods. 151 (1-2): 237-44. -
Levesque, M.A. et al. (1995) Time-resolved immunofluorometric assay of p53 protein.
Clin Chem. 41 (12 Pt 1): 1720-9. -
Carvalho, T. et al. (2009) Immunohistochemical evaluation of vascular urinary bladder tumors from cows with enzootic hematuria.
Vet Pathol. 46 (2): 211-21. -
Phillips, A. et al. (2010) HDMX-L is expressed from a functional p53-responsive promoter in the first intron of the HDMX gene and participates in an autoregulatory feedback loop to control p53 activity.
J Biol Chem. 285 (38): 29111-27. -
Bergman, L.M. et al. (2009) CtBPs promote cell survival through the maintenance of mitotic fidelity.
Mol Cell Biol. 29: 4539-51. -
Hietanen, S. et al. (2000) Activation of p53 in cervical carcinoma cells by small molecules.
Proc Natl Acad Sci U S A. 97 (15): 8501-6. -
Phelps, M. et al. (2003) p53-independent activation of the hdm2-P2 promoter through multiple transcription factor response elements results in elevated hdm2 expression in estrogen receptor alpha-positive breast cancer cells.
Cancer Res. 63: 2616-23.
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