Mucin 2 antibody | 996/1

Mouse anti Human Mucin 2

Product Type
Monoclonal Antibody
Clone
996/1
Isotype
IgG1
Specificity
Mucin 2

Product Code Applications Pack Size List Price Your Price Qty
MCA1743
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * P WB 0.2 mg loader
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loader
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Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes human Mucin 2 (MUC-2), and shows no cross-reactivity with MUC-1, MUC-3 or MUC-4.

In formalin fixed, paraffin embedded tissue sections Mouse anti Human Mucin 2 antibody, clone 996/1 reveals high levels of expression in colon, liver and prostate tissues (Durrant et al. 1994).

Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes malignant colonic mucosa as well as normal mucosa. Epitope mapping indicates that Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes a sequence PTGTQ within the mucin 2 tandem repeat region (Uray et al. 1999).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
MUC-2 tandem repeat peptide.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of a mouse NS0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Normal colon

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Source Reference

  1. Durrant, L.G. et al. (1994) Production of monoclonal antibodies recognising the peptide core of MUC2 intestinal mucin.
    Eur J Cancer. 30A (3): 355-63.

References for Mucin 2 antibody

  1. Filipe, M.I. et al. (1996) Expression of a peptide epitope of the colonic mucin MUC2 in precursor lesions to gastric carcinoma.
    Eur J Cancer Prev. 5 (4): 287-95.
  2. Paulsen, F.P. et al. (2003) Characterization of mucins in human lacrimal sac and nasolacrimal duct.
    Invest Ophthalmol Vis Sci. 44 (5): 1807-13.
  3. Price, M.R. et al. (1999) Separation of distinct MUC2 mucin glycoforms using two anti-peptide monoclonal antibodies.
    Int J Oncol. 15 (4): 803-9.
  4. Price, M.R. et al. (1993) Immune recognition of human colonic-tumour-associated MUC-2 mucins using an anti-peptide antibody.
    Int J Cancer. 55 (5): 753-9.
  5. Tugyi, R. et al. (2005) Partial D-amino acid substitution: Improved enzymatic stability and preserved Ab recognition of a MUC2 epitope peptide.
    Proc Natl Acad Sci U S A. 102 (2): 413-8.
  6. Uray, K. et al. (2000) Effect of D-amino acid substitution in a mucin 2 epitope on mucin-specific monoclonal antibody recognition.
    Arch Biochem Biophys. 378 (1): 25-32.
  7. Uray, K. et al. (1999) Effect of solution conformation on antibody recognition of a protein core epitope from gastrointestinal mucin (MUC2).
    Arch Biochem Biophys. 361 (1): 65-74.

ELISA

Synonyms
MUC-2
RRID
AB_2148835
UniProt
Q02817
Entrez Gene
MUC2
GO Terms
GO:0005515 protein binding
GO:0070702 inner mucus layer
GO:0070703 outer mucus layer
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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