Mucin 2 antibody | 996/1

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Mouse anti Human Mucin 2:Alexa Fluor® 647

Mouse anti Human Mucin 2:FITC

Mouse anti Human Mucin 2

Product Type
Monoclonal Antibody
Clone
996/1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1743A647 F* 100 Tests/1ml
MCA1743F F* 0.1 mg
MCA1743 C F* P WB 0.2 mg
Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes human Mucin 2 (MUC-2), and shows no cross-reactivity with MUC-1, MUC-3 or MUC-4.

In formalin fixed, paraffin embedded tissue sections Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes colon, liver and prostate tissues strongly. The antibody recognizes malignant colonic mucosa as well as normal mucosa. Epitope mapping indicated that Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes a sequence PTGTQ within the mucin 2 tandem repeat region (Uray et al. 1999).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
MUC-2 tandem repeat peptide.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of a mouse NS0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q02817 Related reagents
Entrez Gene
MUC2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0070702 inner mucus layer
GO:0070703 outer mucus layer
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of Mucin 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
Flow Cytometry 1 Neat 1/10
Flow Cytometry 1
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Normal colon

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 100 Tests/1ml F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

ELISA

Product Specific References

Source Reference

  1. Durrant, L.G. et al. (1994) Production of monoclonal antibodies recognising the peptide core of MUC2 intestinal mucin.
    Eur J Cancer. 30A (3): 355-63.

References for Mucin 2 antibody

  1. Filipe, M.I. et al. (1996) Expression of a peptide epitope of the colonic mucin MUC2 in precursor lesions to gastric carcinoma.
    Eur J Cancer Prev. 5 (4): 287-95.
  2. Paulsen, F.P. et al. (2003) Characterization of mucins in human lacrimal sac and nasolacrimal duct.
    Invest Ophthalmol Vis Sci. 44 (5): 1807-13.
  3. Price, M.R. et al. (1999) Separation of distinct MUC2 mucin glycoforms using two anti-peptide monoclonal antibodies.
    Int J Oncol. 15 (4): 803-9.
  4. Price, M.R. et al. (1993) Immune recognition of human colonic-tumour-associated MUC-2 mucins using an anti-peptide antibody.
    Int J Cancer. 55 (5): 753-9.
  5. Tugyi, R. et al. (2005) Partial D-amino acid substitution: Improved enzymatic stability and preserved Ab recognition of a MUC2 epitope peptide.
    Proc Natl Acad Sci U S A. 102 (2): 413-8.
  6. Uray, K. et al. (2000) Effect of D-amino acid substitution in a mucin 2 epitope on mucin-specific monoclonal antibody recognition.
    Arch Biochem Biophys. 378 (1): 25-32.
  7. Uray, K. et al. (1999) Effect of solution conformation on antibody recognition of a protein core epitope from gastrointestinal mucin (MUC2).
    Arch Biochem Biophys. 361 (1): 65-74.