Mucin 2 antibody | 996/1

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry 1
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10μl of the suggested working dilution to label 1x10⁶ cells in 100μl

Histology Positive Control Tissue

Normal human colon

Source Reference

1. Durrant, L.G. et al. (1994) Production of monoclonal antibodies recognising the peptide core of MUC2 intestinal mucin.
   Eur J Cancer. 30A (3): 355-63.

References for Mucin 2 antibody

1. Price, M.R. et al. (1993) Immune recognition of human colonic-tumour-associated MUC-2 mucins using an anti-peptide antibody.
   Int J Cancer. 55 (5): 753-9.
2. Filipe, M.I. et al. (1996) Expression of a peptide epitope of the colonic mucin MUC2 in precursor lesions to gastric carcinoma.
   Eur J Cancer Prev. 5 (4): 287-95.
3. Paulsen, F.P. et al. (2003) Characterization of mucins in human lacrimal sac and nasolacrimal duct.
   Invest Ophthalmol Vis Sci. 44 (5): 1807-13.
4. Price, M.R. et al. (1999) Separation of distinct MUC2 mucin glycoforms using two anti-peptide monoclonal antibodies.
   Int J Oncol. 15 (4): 803-9.
5. Tugyi, R. et al. (2005) Partial D-amino acid substitution: Improved enzymatic stability and preserved Ab recognition of a MUC2 epitope peptide.
   Proc Natl Acad Sci U S A. 102 (2): 413-8.
6. Uray, K. et al. (2000) Effect of D-amino acid substitution in a mucin 2 epitope on mucin-specific monoclonal antibody recognition.
   Arch Biochem Biophys. 378 (1): 25-32.
7. Uray, K. et al. (1999) Effect of solution conformation on antibody recognition of a protein core epitope from gastrointestinal mucin (MUC2).
   Arch Biochem Biophys. 361 (1): 65-74.

ELISA

Synonyms

MUC-2

RRID

AB_2148835

UniProt

Q02817

Entrez Gene

MUC2

GO Terms

GO:0005515 protein binding

GO:0070702 inner mucus layer

GO:0070703 outer mucus layer

MCA1743

148579 153589

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