Mucin 2 antibody | 996/1
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Mouse anti Human Mucin 2
- Product Type
- Monoclonal Antibody
- Mucin 2
|Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes human Mucin 2 (MUC-2), and shows no cross-reactivity with MUC-1, MUC-3 or MUC-4.
In formalin fixed, paraffin embedded tissue sections Mouse anti Human Mucin 2 antibody, clone 996/1 reveals high levels of expression in colon, liver and prostate tissues (Durrant et al. 1994).
Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes malignant colonic mucosa as well as normal mucosa. Epitope mapping indicates that Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes a sequence PTGTQ within the mucin 2 tandem repeat region (Uray et al. 1999).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- MUC-2 tandem repeat peptide.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of a mouse NS0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1|
|Immunohistology - Frozen|
|Immunohistology - Paraffin|
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
- Histology Positive Control Tissue
- Normal human colon
Durrant, L.G. et al. (1994) Production of monoclonal antibodies recognising the peptide core of MUC2 intestinal mucin.
Eur J Cancer. 30A (3): 355-63.
References for Mucin 2 antibody
Price, M.R. et al. (1993) Immune recognition of human colonic-tumour-associated MUC-2 mucins using an anti-peptide antibody.
Int J Cancer. 55 (5): 753-9.
Filipe, M.I. et al. (1996) Expression of a peptide epitope of the colonic mucin MUC2 in precursor lesions to gastric carcinoma.
Eur J Cancer Prev. 5 (4): 287-95.
Paulsen, F.P. et al. (2003) Characterization of mucins in human lacrimal sac and nasolacrimal duct.
Invest Ophthalmol Vis Sci. 44 (5): 1807-13.
Price, M.R. et al. (1999) Separation of distinct MUC2 mucin glycoforms using two anti-peptide monoclonal antibodies.
Int J Oncol. 15 (4): 803-9.
Tugyi, R. et al. (2005) Partial D-amino acid substitution: Improved enzymatic stability and preserved Ab recognition of a MUC2 epitope peptide.
Proc Natl Acad Sci U S A. 102 (2): 413-8.
Uray, K. et al. (2000) Effect of D-amino acid substitution in a mucin 2 epitope on mucin-specific monoclonal antibody recognition.
Arch Biochem Biophys. 378 (1): 25-32.
Uray, K. et al. (1999) Effect of solution conformation on antibody recognition of a protein core epitope from gastrointestinal mucin (MUC2).
Arch Biochem Biophys. 361 (1): 65-74.
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