MCP-1 antibody | 2.2-4H5-1A11
Mouse anti Human MCP-1
- Product Type
- Monoclonal Antibody
- Clone
- 2.2-4H5-1A11
- Isotype
- IgG1
- Specificity
- MCP-1
Antibody Quality and Performance Guarantee
| Mouse anti Human MCP-1 antibody, clone 2.2-4H5-1A11 recognizes human C-C motif chemokine 2, also known as Monocyte chemoattractant protein 1 (MCP-1), Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Small-inducible cytokine A2 or CCL2, and does not cross react with MCP-2, MCP-3 or other structurally related chemokines. MCP-1 is a 99 amino acid, including a 23 aa signal peptide ~13 kDa chemotactic protein, a chemoattractant for monocytes and basophils but not neutrophils or eosinophils (Weber et al.1996). MCP-1 is produced by a number of cell types, including monocytes, macrophages, microglia, fibroblasts and epithelial cells (Cushing et al. 1990). MCP-1 influences T-cell immunity polarizing responses towards the Th2 phenotype (Karpus and Kennedy 1997). Variations in the CCL2 gene affects susceptibility to Mycobacteriun tuberculosis infection (Flores-Villanueva et al. 2005). Mouse anti Human MCP-1 antibody, clone 2.2-4H5-1A11 recognizes both native MCP-1 and recombinant MCP-1 |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human MCP-1/MCAF ( PHP061)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Fusion Partners
- Spleen cells from immunized BALB/c X ICR F1 mice were fused with cells of a mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 | 2.0ug/ml |
| Immunohistology - Paraffin 1 | |
10ug/ml | |
| Western Blotting | |
0.2 | 0.4ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with PHP061 as the standard.
- Histology Positive Control Tissue
- Human Breast Invasive Ductal Carcinoma
- Western Blotting
- This product may be used under either reducing or non-reducing conditions together with PHP061 as the positive control.
References for MCP-1 antibody
-
Johansson, U. et al. (2008) Formation of composite endothelial cell-mesenchymal stem cell islets: a novel approach to promote islet revascularization.
Diabetes. 57: 2393-401. -
Nio-Kobayashi J et al. (2015) Regulated C-C motif ligand 2 (CCL2) in luteal cells contributes to macrophage infiltration into the human corpus luteum during luteolysis.
Mol Hum Reprod. pii: gav028. -
Chuang, L.P. et al. (2016) Increased MCP-1 gene expression in monocytes of severe OSA patients and under intermittent hypoxia.
Sleep Breath. 20 (1): 425-33.
- Synonyms
- CCL2
- RRID
- AB_2071556
- UniProt
- P13500
- Entrez Gene
- CCL2
- GO Terms
- GO:0002548 monocyte chemotaxis
- GO:0007155 cell adhesion
- GO:0004672 protein kinase activity
- GO:0004871 signal transducer activity
- GO:0005615 extracellular space
- GO:0006468 protein phosphorylation
- GO:0006874 cellular calcium ion homeostasis
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- View More GO Terms
- GO:0006959 humoral immune response
- GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger
- GO:0007259 JAK-STAT cascade
- GO:0008009 chemokine activity
- GO:0019079 viral genome replication
- GO:0031727 CCR2 chemokine receptor binding
- GO:0048246 macrophage chemotaxis
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170509
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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