MCP-1 antibody | 2.2-4H5-1A11

Mouse anti Human MCP-1

Product Type
Monoclonal Antibody
Clone
2.2-4H5-1A11
Isotype
IgG1
Specificity
MCP-1

Product Code Applications Pack Size List Price Your Price Qty
MCA2486
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.5 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human MCP-1 antibody, clone 2.2-4A4-1A11 recognizes human C-C motif chemokine 2, also known as Monocyte chemoattractant protein 1 (MCP-1), Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Small-inducible cytokine A2 or CCL2, and does not cross react with MCP-2, MCP-3 or other structurally related chemokines. MCP-1 is a 99 amino acid, including a 23 aa signal peptide ~13 kDa chemotactic protein, a chemoattractant for monocytes and basophils but not neutrophils or eosinophils (Weber et al.1996).

MCP-1 is produced by a number of cell types, including monocytes, macrophages, microglia, fibroblasts and epithelial cells (Cushing et al. 1990). MCP-1 influences T-cell immunity polarizing responses towards the Th2 phenotype (Karpus and Kennedy 1997). Variations in the CCL2 gene affects susceptibility to Mycobacteriun tuberculosis infection (Flores-Villanueva et al. 2005).

Mouse anti Human MCP-1 antibody, clone 2.2-4A4-1A11 recognizes both native and recombinant MCP-1 in conjunction with Rabbit anti Human MCP-1 polyclonal antibody in a sensitive sandwich ELISA assay.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human MCP-1/MCAF ( PHP061)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Fusion Partners
Spleen cells from immunized BALB/c X ICR F1 mice were fused with cells of a mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 2.0ug/ml
Immunohistology - Paraffin 1 10ug/ml
Western Blotting 0.2 0.4ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AHP784B as the detection reagent and PHP061 as the standard.
Histology Positive Control Tissue
Human Breast Invasive Ductal Carcinoma
Western Blotting
This product may be used under either reducing or non-reducing conditions together with PHP061 as the positive control.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (Fc):HRP
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)

References for MCP-1 antibody

  1. Johansson, U. et al. (2008) Formation of composite endothelial cell-mesenchymal stem cell islets: a novel approach to promote islet revascularization.
    Diabetes. 57: 2393-401.
  2. Nio-Kobayashi J et al. (2015) Regulated C-C motif ligand 2 (CCL2) in luteal cells contributes to macrophage infiltration into the human corpus luteum during luteolysis.
    Mol Hum Reprod. pii: gav028.
  3. Chuang, L.P. et al. (2016) Increased MCP-1 gene expression in monocytes of severe OSA patients and under intermittent hypoxia.
    Sleep Breath. 20 (1): 425-33.

Synonyms
CCL2
RRID
AB_2071556
UniProt
P13500
Entrez Gene
CCL2
GO Terms
GO:0002548 monocyte chemotaxis
GO:0007155 cell adhesion
GO:0004672 protein kinase activity
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006468 protein phosphorylation
GO:0006874 cellular calcium ion homeostasis
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0006959 humoral immune response
GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger
GO:0007259 JAK-STAT cascade
GO:0008009 chemokine activity
GO:0019079 viral genome replication
GO:0031727 CCR2 chemokine receptor binding
GO:0048246 macrophage chemotaxis
View more products with MCP-1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products