MCP-1

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Sandwich ELISA analysis of human MCP-1 using Mouse anti Human MCP-1 (MCA2486) as a capture reagent and biotinylated Rabbit anti Human MCP-1 (AHP784B) as a detection reagent with recombinant human MCP-1 (PHP061) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum (green) and plasma (orange) samples are included undiluted

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  • Recombinant Human MCP-1/Mcaf
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP061E, FN, WBdatasheet pdfdatasheet pdf20 µg
    PHP061
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    References
    Reviews
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    • Recombinant human MCP-1/MCAF also known as C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemoattractant protein 1, HC11 or Small-inducible cytokine A2 is encoded by the CCL2 gene. Recombinant MCP-1 is a 76 amino acid ~13kDa chemokine involved in chemotaxis and polarization of the immune response.

      Recombinant human MCP-1/MCAF has been employed successfully as a control antigen in the development of a sensitive ELISA employing Mouse anti Human MCP-1 antibody clone 2.2-4A4-1A11 as a capture reagent and biotinylated Rabbit anti Human MCP-1 polyclonal antibody as a detection reagent.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays10ng/ml100ng/ml
      Western Blotting1.53.0ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used as a standard for ELISA applications with AHP784, AHP784B or MCA2486.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      This product may be used as the positive control for Western Blotting applications with AHP784, AHP784B or MCA2486.
    • Instructions For Use

    Additional MCP-1 Formats

    Formats Applications Sizes available
    MCP-1 : Rec. Protein E, FN, WB 20 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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