MBP antibody | 22
Mouse anti MBP antibody, clone 22 recognizes an epitope between amino acids 84 and 89 of human myelin basic protein, also known as MBP, Myelin A1 protein or Myelin membrane encephalitogenic protein. MBP is a 304 amino acid ~33 kDa abundant component of the myelin membrane. There are 6 potential isoforms of MBP generated by alternative splicing, 4 of which lack the N-terminal region containing the immunogen sequence. Mouse anti MBP antibody, clone 22 is expected to recognize isoforms 1 and 2 (UniProt: P02686). The N-terminal region of MBP is highly conserved and Mouse anti MBP antibody, clone 22 is expected to be broadly species cross reactive for isoforms maintaining the N-terminal region. Mouse anti MBP antibody, clone 22 has been reported as suitable for use in western blotting (Chignola et al. 2000).
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
0.05% Sodium Azide
- Human myelin basic protein.
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- P02686 Related reagents
- Entrez Gene
- MBP Related reagents
- For research purposes only
Applications of MBP antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin|
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for MBP antibody
Groome, N. P. et al. (1988) New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein.
J. Neuroimmunol 19: 305-315.
Martenson, R. E. (1984) Experimental allergic encephalomyelitis. A useful model of multiple sclerosis.
Eds Alvord, Kies and Suckling pp 511-521. Alan Liss N.Y.
Harrower, T.P. et al. (2006) Long-term survival and integration of porcine expanded neural precursor cell grafts in a rat model of Parkinson's disease.
Exp Neurol. 197: 56-69.
Liao, M.C. et al. (2009) Degradation of amyloid beta protein by purified myelin basic protein.
J Biol Chem. 284: 28917-25.
Diemel, L.T. et al. (2003) Role for TGF-beta1, FGF-2 and PDGF-AA in a myelination of CNS aggregate cultures enriched with macrophages.
J Neurosci Res. 74 (6): 858-67.