Mouse anti MBP antibody, clone 22
recognizes an epitope between amino acids 84 and 89 of human myelin basic protein, also known as MBP, Myelin A1 protein or Myelin membrane encephalitogenic protein. MBP is a 304 amino acid ~33 kDa abundant component of the myelin membrane. There are 6 potential isoforms of MBP generated by alternative splicing, 4 of which lack the N-terminal region containing the immunogen sequence. Mouse anti MBP antibody, clone 22 is expected to recognize isoforms 1 and 2 (UniProt: P02686
). The N-terminal region of MBP is highly conserved and Mouse anti MBP antibody, clone 22 is expected to be broadly species cross reactive for isoforms maintaining the N-terminal region. Mouse anti MBP antibody, clone 22 has been reported as suitable for use in western blotting (Chignola et al. 2000
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- Human myelin basic protein.
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MBP antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
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Secondary Antibodies Available
Application Based External Images
Product Specific References
References for MBP antibody
Groome, N. et al. (1988) New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein.
J Neuroimmunol. 19 (4): 305-15.
Martenson, R. E. (1984) Experimental allergic encephalomyelitis. A useful model of multiple sclerosis.
Eds Alvord, Kies and Suckling pp 511-521. Alan Liss N.Y.
Harrower, T.P. et al. (2006) Long-term survival and integration of porcine expanded neural precursor cell grafts in a rat model of Parkinson's disease.
Exp Neurol. 197 (1): 56-69.
Liao, M.C. et al. (2009) Degradation of amyloid beta protein by purified myelin basic protein.
J Biol Chem. 284: 28917-25.
Diemel, L.T. et al. (2003) Role for TGF-beta1, FGF-2 and PDGF-AA in a myelination of CNS aggregate cultures enriched with macrophages.
J Neurosci Res. 74 (6): 858-67.