MBP antibody | 22

Mouse anti Human MBP (aa84-89)

Product Type
Monoclonal Antibody
Clone
22
Isotype
IgG2b
Specificity
MBP
Region
(aa84-89)

Product Code Applications Pack Size List Price Your Price Qty
MCA686S
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SDS Safety Datasheet SDS
C P 2 ml loader
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loader
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Mouse anti MBP antibody, clone 22 recognizes an epitope between amino acids 84 and 89 of human myelin basic protein, also known as MBP, Myelin A1 protein or Myelin membrane encephalitogenic protein. MBP is a 304 amino acid ~33 kDa abundant component of the myelin membrane. There are 6 potential isoforms of MBP generated by alternative splicing, 4 of which lack the N-terminal region containing the immunogen sequence. Mouse anti MBP antibody, clone 22 is expected to recognize isoforms 1 and 2 (UniProt: P02686). The N-terminal region of MBP is highly conserved and Mouse anti MBP antibody, clone 22 is expected to be broadly species cross reactive for isoforms maintaining the N-terminal region. Mouse anti MBP antibody, clone 22 has been reported as suitable for use in western blotting (Chignola et al. 2000).

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Human myelin basic protein.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for MBP antibody

  1. Chignola, R. et al. (2000) Expression of myelin basic protein (MBP) epitopes in human non-neural cells revealed by two anti-MBP IgM monoclonal antibodies.
    Clin Exp Immunol. 122 (3): 429-36.
  2. Groome, N. et al. (1988) New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein.
    J Neuroimmunol. 19 (4): 305-15.
  3. Martenson, R. E. (1984) Experimental allergic encephalomyelitis. A useful model of multiple sclerosis.
    Eds Alvord, Kies and Suckling pp 511-521. Alan Liss N.Y.
  4. Harrower, T.P. et al. (2006) Long-term survival and integration of porcine expanded neural precursor cell grafts in a rat model of Parkinson's disease.
    Exp Neurol. 197 (1): 56-69.
  5. Liao, M.C. et al. (2009) Degradation of amyloid beta protein by purified myelin basic protein.
    J Biol Chem. 284: 28917-25.
  6. Diemel, L.T. et al. (2003) Role for TGF-beta1, FGF-2 and PDGF-AA in a myelination of CNS aggregate cultures enriched with macrophages.
    J Neurosci Res. 74 (6): 858-67.

Western Blotting

Synonyms
Myelin Basic Protein
RRID
AB_325006
UniProt
P02686
Entrez Gene
MBP
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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