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MBP antibody | 22

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Anti Human MBP (aa84-89) Antibody, clone 22 gallery image 1
Staining of white matter in human brain cryosection with Mouse anti MBP antibody, clone 22 (MCA686S).

Mouse anti Human MBP (aa84-89)

Product Type
Monoclonal Antibody
Clone
22
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA686S
Datasheet
C P
MSDS
2 ml loader

Mouse anti MBP antibody, clone 22 recognizes an epitope between amino acids 84 and 89 of human myelin basic protein, also known as MBP, Myelin A1 protein or Myelin membrane encephalitogenic protein. MBP is a 304 amino acid ~33 kDa abundant component of the myelin membrane. There are 6 potential isoforms of MBP generated by alternative splicing, 4 of which lack the N-terminal region containing the immunogen sequence. Mouse anti MBP antibody, clone 22 is expected to recognize isoforms 1 and 2 (UniProt: P02686). The N-terminal region of MBP is highly conserved and Mouse anti MBP antibody, clone 22 is expected to be broadly species cross reactive for isoforms maintaining the N-terminal region. Mouse anti MBP antibody, clone 22 has been reported as suitable for use in western blotting (Chignola et al. 2000).

Product Details

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.05%Sodium Azide
Immunogen
Human myelin basic protein.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P02686 Related reagents
Entrez Gene
MBP Related reagents
Regulatory
For research purposes only

Applications of MBP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F F 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Western Blotting

Product Specific References

References for MBP antibody

  1. Groome, N. et al. (1988) New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein.
    J Neuroimmunol. 19 (4): 305-15.
  2. Martenson, R. E. (1984) Experimental allergic encephalomyelitis. A useful model of multiple sclerosis.
    Eds Alvord, Kies and Suckling pp 511-521. Alan Liss N.Y.
  3. Harrower, T.P. et al. (2006) Long-term survival and integration of porcine expanded neural precursor cell grafts in a rat model of Parkinson's disease.
    Exp Neurol. 197 (1): 56-69.
  4. Liao, M.C. et al. (2009) Degradation of amyloid beta protein by purified myelin basic protein.
    J Biol Chem. 284: 28917-25.
  5. Diemel, L.T. et al. (2003) Role for TGF-beta1, FGF-2 and PDGF-AA in a myelination of CNS aggregate cultures enriched with macrophages.
    J Neurosci Res. 74 (6): 858-67.
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