Lysozyme antibody | BGN/0696/5B1

Mouse anti Human Lysozyme

Product Type
Monoclonal Antibody
Clone
BGN/0696/5B1
Isotype
IgG2a
Specificity
Lysozyme

Product Code Applications Pack Size List Price Your Price Qty
0100-0523
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * P * 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human Lysozyme antibody, clone BGN/0696/5B1 recognizes lysozyme, an enzyme that is present in mucosal secretions such as saliva and tears and is involved with non-specific organism defence. It functions by hydrolyzing 1,2-beta linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues such as those found in the walls of various Gram-positive bacteria.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Native, from urine.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/10
Immunohistology - Paraffin 2
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human Tonsil

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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

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RRID
AB_617338
UniProt
P61626
Entrez Gene
LYZ
GO Terms
GO:0005515 protein binding
GO:0003796 lysozyme activity
GO:0042742 defense response to bacterium
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0019835 cytolysis
GO:0016998 cell wall macromolecule catabolic process
View more products with LYSOZYME specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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