INI-1 antibody | 3E10

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Mouse anti Human INI-1:Preservative Free

Product Type
Monoclonal Antibody
Clone
3E10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA3457Z P* WB 0.1 mg
Mouse anti Human INI-1 antibody, clone 3E10 recognizes human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, also known as INI-1, BRG1-associated factor 47, BAF47, SMARCB1, Integrase interactor 1 protein or SNF5 homolog. INI-1 is a 385 amino acid ~46 kDa component of the BAF complex involved in chromatin remodelling. INI-1 also acts as a tumor supressor.

Mutations in the SMARCB1 gene can lead to hereditary cancer syndromes including Rhabdoid tumor predisposition syndrome 1 (RTPS1) with presentation of renal or nervous system tumors or Schwannomatosis 1 (SWNTS1) with development of non-vestibular Schwannomas. Mutation in the SMARCB1 gene can also lead to Mental retardation, autosomal dominant 15 (MRD15) characterized by multiple developmental anomalies, mental retardation and hearing loss (Tsurusaki et al. 2012).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 81-181 of human INI-1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q12824 Related reagents
Entrez Gene
SMARCB1 Related reagents
GO Terms
GO:0000228 nuclear chromosome
GO:0002039 p53 binding
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006338 chromatin remodeling
GO:0006350 transcription
GO:0007049 cell cycle
GO:0007399 nervous system development
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0015074 DNA integration
GO:0016514 SWI/SNF complex
GO:0044419 interspecies interaction between organisms
GO:0045090 retroviral genome replication
GO:0051091 positive regulation of transcription factor activity
GO:0071564 npBAF complex
GO:0071565 nBAF complex
Regulatory
For research purposes only

Applications of INI-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 0.1 10 ug/ml
Western Blotting 0.1 10 ug/ml
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F