IgG antibody | 8A4
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Mouse anti Human IgG (Fc) (CH2 Domain)
- Product Type
- Monoclonal Antibody
- Clone
- 8A4
- Isotype
- IgG1
- Specificity
- IgG
- Region
- (Fc) (CH2 DOMAIN)
Antibody Quality and Performance Guarantee
| Mouse anti Human IgG (Fc) (CH2 Domain) antibody, clone 8A4 recognizes an epitope within the CH2 domain of the Fc region of all subclasses of human IgG. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Human IgG polyclonal.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mouse were fused with cells of the P3-NS1/Ag1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
1/50 | 1/100 |
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
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Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
References for IgG antibody
-
Komatsuda, A. et al. (2008) Monoclonal immunoglobulin deposition disease associated with membranous features.
Nephrol. Dial. Transplant. 23: 3888-94. -
Komatsuda, A. et al. (2013) Proliferative glomerulonephritis with discrete deposition of monoclonal immunoglobulin γ1 C(H) 2 heavy chain and κ light chain: A new variant of monoclonal immunoglobulin deposition disease.
Pathol Int. 63: 63-7. -
Gehlsen, K. et al. (2012) Pharmacokinetics of engineered human monomeric and dimeric CH2 domains.
MAbs. 4: 466-74. -
Kato, H. et al. (2015) Rapid Deterioration of the Renal Function Caused by the Coexistence of Intratubular Amyloidosis and Myeloma Cast Nephropathy.
Intern Med. 54 (23): 3023-8.
- RRID
- AB_770116
- UniProt
- P01857
- P01859
- P01860
- P01861
- Entrez Gene
- IGHG1
- IGHG2
- IGHG3
- IGHG4
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0006955 immune response
MCA2477G
149126
163370
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