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IgA1 antibody | B3506B4

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Anti Human IgA1 Alpha 1 Chain Antibody, clone B3506B4 thumbnail image 1 Anti Human IgA1 Alpha 1 Chain Antibody, clone B3506B4 thumbnail image 2
Anti Human IgA1 Alpha 1 Chain Antibody, clone B3506B4 gallery image 1
Published customer image:
HRP conjugated Mouse anti Human IgA1 antibody, clone B3506B4 (913005) used for the evaluation of IgA1 levels in human serum samples by ELISA.
Image caption:
Comparison of serum IgA in patients with IgA nephropathy and heathy subjects. J-IgA: joining chain-containing IgA; J-IgA1: joining chain-containing IgA1; IgAN: IgA nephropathy; NC: normal control. Statistically significant differences were tested with Mann–Whitney U-test.

From: Li G, Wang X, Yang Z, Zhao Q, Wen Y, Li X, Gao R.
Serum Levels of Joining Chain-Containing IgA1 Are Not Elevated in Patients with IgA Nephropathy.
Dis Markers. 2019 Jul 2;2019:9802839.
doi: 10.1155/2019/9802839.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human IgA1 Alpha 1 Chain Antibody, clone B3506B4 gallery image 2
Published customer image:
HRP conjugated Mouse anti Human IgA1 antibody, clone B3506B4 (913005) used for the evaluation of IgA1 levels in plasma samples by western blotting.
Image caption:
Verification of successful preparation of ddIgA1 and IgG-dd-IgA1 complexes. a Glycosylation status of IgA1 and ddIgA1 were detected by two lectins, SNA and VVL, which recognized sialic acid (SA), and N-Acetylgalactosamine (GalNAc), respectively. IgA1 molecules showed high binding capacity to SNA (blank column) and low binding capacity to VVL (filled column). In contrary, ddIgA1 showed low binding capacity to SNA (blank column) and high binding capacity to VVL (filled column). b Existence of dd-IgA1 (upper panel) and IgG (lower panel) in IgG-ddIgA1 complexes were tested by Western blot analysis. Both IgA1 and IgG were existence in IgG-ddIgA1 complexes of recruited individuals (lane 1–4). Isolated ddIgA1 (lane 5), IgA1 (lane 6) and IgG (lane 7) were used as references

From: Zhao YF, Zhu L, Liu LJ, Shi SF, Lv JC, Zhang H.
Pathogenic role of glycan-specific IgG antibodies in IgA nephropathy.
BMC Nephrol. 2017 Sep 29;18(1):301.
doi: 10.1186/s12882-017-0722-3.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human IgA1 Alpha 1 Chain:Alk. Phos.

Mouse anti Human IgA1 Alpha 1 Chain:Biotin

Mouse anti Human IgA1 Alpha 1 Chain:HRP

Mouse anti Human IgA1 Alpha 1 Chain

Product Type
Monoclonal Antibody
Clone
B3506B4
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
913004
Datasheet
C E IB WB
SDS
1 ml loader

913008
Datasheet
E IB WB
SDS
0.5 mg loader

913005
Datasheet
E
SDS
1 ml loader

913001
Datasheet
C E IB WB
SDS
0.5 mg loader

Mouse anti Human IgA1 antibody, clone B3506B4 recognizes the Fc portion of the heavy chain of human IgA1 as demonstrated by ELISA.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared from ascites
Preparation
Purified IgG prepared from ascites
Preparation
Purified IgG prepared from ascites
Preparation
Purified IgG prepared from ascites
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.1% Sodium azide
50% Glycerol
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
50% glycerol.
Preservative Stabilisers
None present
Immunogen
Human IgA1 heavy chains.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P01876
Entrez Gene
IGHA1
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0006955 immune response
GO:0018298 protein-chromophore linkage
Regulatory
For research purposes only

Applications of IgA1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunoblotting
Immunohistology - Frozen
Western Blotting
ELISA
Immunoblotting
Western Blotting
ELISA 1/500 1/2000
ELISA
Immunoblotting
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for IgA1 antibody

  1. Zhao, Y.F. et al. (2017) Pathogenic role of glycan-specific IgG antibodies in IgA nephropathy.
    BMC Nephrol. 18 (1): 301.
  2. Li, G. et al. (2019) Serum Levels of Joining Chain-Containing IgA1 Are Not Elevated in Patients with IgA Nephropathy.
    Dis Markers. 2019: 9802839.

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