HLA DR antibody | L243
FITC conjugated Mouse anti Human HLA-DR antibody, clone L243 (MCA2812F) used to evaluate MHC class II expression on bone-marrow derived mesenchymal stem cells by flow cytometry
Image caption:
Chaturvedi CP, Tripathy NK, Minocha E, Sharma A, Rahman K, Nityanand S.
Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia. Stem Cells Int. 2018 Oct 17;2018:6901761.
From: Insert article citation with space after colon and Hyperlink to PubMed record.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human HLA DR
- Product Type
- Monoclonal Antibody
- Clone
- L243
- Isotype
- IgG2a
- Specificity
- HLA DR
Antibody Quality and Performance Guarantee
| Mouse anti Human HLA DR antibody, clone L243 recognizes the human HLA DR antigen, a member of the MHC class II family, which is expressed by B cells, activated T cells and monocytes. Mouse anti Human HLA DR antibody, clone L243 binds to a conformational epitope on HLA DRα and does not cross react with HLA DQ and HLA DP. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chimpanzee Baboon Cynomolgus monkey Rhesus Monkey Marmoset African green monkey Dog Squirrel monkey Pig-tailed macaque Marmoset - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| Description | Mouse IgG2a Negative Control | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
References for HLA DR antibody
-
Wang, D. et al. (2002) The influence of HLA-DR4 (0401) on the immune response to type II collagen and the development of collagen induced arthritis in mice.
J Autoimmun. 18 (2): 95-103. -
Chaturvedi, C.P. et al. (2018) Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.
Stem Cells Int. 2018: 6901761. -
Kim, S.H. et al. (2019) Forkhead box O1 (FOXO1) controls the migratory response of Toll-like receptor (TLR3)-stimulated human mesenchymal stromal cells.
J Biol Chem. 294 (21): 8424-37. -
Temme, S. et al. (2019) Assembly, Intracellular Transport, and Release of MHC Class II Peptide Receptors.
Methods Mol Biol. 1988: 297-314. -
Ngugi, S. et al. (2022) The innate immune response in the marmoset during the acute pneumonic disease caused by Burkholderia pseudomallei.
Infect Immun. : IAI0055021. -
Nelson, M. et al. (2021) Comparison of experimental MERS-CoV infection acquired by three individual routes of infection in the common marmoset.
J Virol. : JVI0173921.
- UniProt
- Q29769
MCA2812GA
152439
159647
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