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Human Seroblock

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Human Seroblock gallery image 1
Staining of THP-1 cells with FITC labelled Mouse anti Human CD11a (MCA1848) or Mouse IgG2a Negative Control:FITC (MCA929F) in the absence (A) and presence (B) of Human SeroBlock (BUF070A). Antibody and isotype control were both used neat. Blue shaded areas represents cells stained with the CD11a antibody while the clear areas represents cells stained with isotype control

Human Seroblock

Product Type
Accessory Reagent
Product Code Applications Pack Size List Price Quantity
BUF070A
Datasheet
F
MSDS
50 Test

BUF070B
Datasheet
F
MSDS
200 Test

Human Fc receptors are expressed on a variety of immune cell types including monocytes, macrophages, B cells, granulocytes and dendritic cells. Cells that express Fc receptors can give false positive immunofluorescent staining due to the Fc receptors binding of Ig. Human SeroBlock is designed to prevent such non-specific staining without interfering with appropriate target staining. Human SeroBlock is compatible with use of anti-human antibodies targeting Fc receptors in flow cytometry.

Product Details

Target Species
Human
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

Regulatory
For research purposes only

Applications of Human Seroblock

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *See Instructions For Use
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

1) Add 5 ul of Human SeroBlock per 100ul cell suspension for 5-10 minutes at room temperature.

2) Add test antibody according to manufacturers instructions – Do not wash Human SeroBlock off the cells. Human SeroBlock is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*Human SeroBlock is also compatible with flow cytometric analysis of human Fc receptors.

3) Proceed with staining as usual.

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