Cytokeratin antibody | C-11
Mouse anti pan Cytokeratin antibody, clone C-11 (MCA6434GA) used to label cytokeratins in bovine cells by immunofluorescence.
Image caption:
Adherence patterns of EDL933 strains and mutants, obtained from sources A, B, and C, on RSE cells. The immunofluorescent stained slides are shown at 40x magnification. O157 have green fluorescence; RSE cells’ cytokeratins have orange-red fluorescence, and nuclei have blue fluorescence.
From: Hoefler, R.S. & Kudva, I.T.
EDL933 Strains of Escherichia coli O157 can Demonstrate Genetic Diversity and Differential Adherence to Bovine Recto-Anal Junction Squamous Epithelial Cells.
The Open Microbiological Journal (2021) 15: 129-38.
10.2174/1874285802115010129.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti human cytokeratin (Pan reactive) antibody, clone C-11 (MCA6434) used to label cytokeratins in bovine recto-anal junction tissue sections by immunofluorescence.
Image caption:
Immunofluorescent images of tissue sections from RAJ-IVOC adherence assay trial 1. The RAJ-IVOC were inoculated with either O157 (106 CFU inoculum as shown in parenthesis) or not inoculated (no bacteria) and incubated at 39 °C for 3 or 24 h. Tissue sections of the RAJ-IVOC were then stained with immunofluorescent antibodies targeting the RAJ cells’ cytokeratins and O157, and images were recorded at 100× magnification. The adherent bacteria (shown with arrows), RAJ cells’ cytokeratins, and the nuclei have green, orange–red, and blue fluorescence, respectively. The squamous (S), junction (J, boxed), and columnar (C) regions of the RAJ are indicated along with a 100 μm scale bar.
From: Kudva IT, Biernbaum EN, Cassmann ED, Palmer MV.
Bovine Rectoanal Junction In Vitro Organ Culture Model System to Study Shiga Toxin-Producing Escherichia coli Adherence.
Microorganisms. 2023 May 15;11(5):1289.
doi: 10.3390/microorganisms11051289.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Cytokeratin (Pan Reactive)
- Product Type
- Monoclonal Antibody
- Clone
- C-11
- Isotype
- IgG1
- Specificity
- Cytokeratin
- Region
- PAN REACTIVE
Antibody Quality and Performance Guarantee
MCA6434GA
| Mouse anti human cytokeratin (Pan reactive) antibody, clone C-11 recognizes the human cytokeratins 4, 5, 6, 8, 10, 13 and 18. Cytokeratins, also known as keratins, form a subfamily of cytoplamsic proteins expressed in epithelial cells where they form intermediate filaments responsible for providing cells resistance to mechanical stress. Cytokeratins are characterized by their remarkable biochemical diversity, with epithelial tissues expressing at least 20 different cytokeratins (cytokeratin 1 to 20) divided into type I cytokeratins (acidic cytokeratins 9 to 20) and type II cytokeratins (basic to neutral cytokeratins 1 to 8) subfamilies. Cytokeratin expression profile is largely specific to particular organs and tissues and their development stage. That tissue-specific expression profile of cytokeratins is usually retained upon epithelial cell transformation and is therefore used in clinic to identify the cells from which carcinomas originated from. Mouse anti Human Cytokeratin (Pan reactive) antibody, clone C-11, has been validated for use on the Genesis Cell Isolation System with the CelSelect SlideTM technology. |
|
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mammals Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Keratin-enriched preparation from human epidermoid carcinoma cell line A431
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- CelSelect Slides is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/100 | |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 2 | |
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| Immunoprecipitation | |
||
| Rare Cell / CTC Enumeration | |
1/200 | |
| Western Blotting | |
- 1Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
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Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Bártek, J. et al. (1991) A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology.
J. Pathol. 164: 215-24.
References for Cytokeratin antibody
-
Bártková, J.et al. (1991) Effects of tissue fixation conditions and protease pretreatment on immunohistochemical performance of a large series of new anti-keratin monoclonal antibodies: value in oncopathology.
Neoplasma. 38:439-446. -
Hoefler, R.S. & , Kudva, I.T. (2021) EDL933 Strains of Escherichia coli O157 can Demonstrate Genetic Diversity and Differential Adherence to Bovine Recto-Anal Junction Squamous Epithelial Cells
The Open Microbiology Journal. 15 (1): 129-38. -
Kudva, I.T. et al. (2023) Bovine Rectoanal Junction In Vitro Organ Culture Model System to Study Shiga Toxin-Producing Escherichia coli Adherence.
Microorganisms. 11 (5): 1289.
- UniProt
- P02538
- P05783
- P13645
- P13647
- P13646
- P19013
- P48668
- P04259
- P05787
- Entrez Gene
- KRT6A
- KRT6B
- KRT18
- KRT8
- KRT10
- KRT13
- KRT5
- KRT4
- KRT6C
- GO Terms
- GO:0005515 protein binding
- GO:0005200 structural constituent of cytoskeleton
- GO:0007398 ectoderm development
- GO:0008284 positive regulation of cell proliferation
- GO:0030154 cell differentiation
- GO:0045095 keratin filament
- GO:0005198 structural molecule activity
- GO:0007049 cell cycle
- GO:0009653 anatomical structure morphogenesis
- View More GO Terms
- GO:0034451 centriolar satellite
- GO:0043000 Golgi to plasma membrane CFTR protein transport
- GO:0043066 negative regulation of apoptosis
- GO:0044419 interspecies interaction between organisms
- GO:0048471 perinuclear region of cytoplasm
- GO:0008544 epidermis development
- GO:0030280 structural constituent of epidermis
- GO:0005882 intermediate filament
- GO:0005829 cytosol
- GO:0031581 hemidesmosome assembly
- GO:0007010 cytoskeleton organization
- GO:0030855 epithelial cell differentiation
- GO:0050680 negative regulation of epithelial cell proliferation
MCA6434GA
166889
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