Cytochrome B245 antibody | CS9

Mouse anti Human Cytochrome B245 Light Chain

Product Type
Monoclonal Antibody
Clone
CS9
Isotype
IgG1
Specificity
Cytochrome B245
Region
LIGHT CHAIN

Product Code Applications Pack Size List Price Your Price Qty
MCA4686
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F * IP WB 0.2 mg loader
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loader
MCA4686GA
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SDS Safety Datasheet SDS
C E F * IP WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 recognizes the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections.

The light chain (p22phox), along with the heavy chain (gp91phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons.

The core region of the CS9 epitope is amino acids 165 - 169 (KKPSE), a cytosolic epitope on p22phox not accessible on intact neutrophils. Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 also has an inhibitory effect on NADPH oxidase activation.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Cytochrome B245 solubilised in octyl-beta-gluycopyranoside.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the 3PU1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/10 1/100
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Western Blotting
Mouse anti Human cytochrome B245 antibody, clone CS9 detects a band of approximately 19kDa in neutrophil membrane extracts

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Synonyms
p22phox
RRID
AB_1935435
UniProt
P13498
Entrez Gene
CYBA
GO Terms
GO:0009055 electron carrier activity
GO:0030141 stored secretory granule
GO:0022900 electron transport chain
GO:0006810 transport
GO:0006954 inflammatory response
GO:0014895 smooth muscle hypertrophy
GO:0017004 cytochrome complex assembly
GO:0017124 SH3 domain binding
GO:0020037 heme binding
GO:0042554 superoxide anion generation
GO:0043020 NADPH oxidase complex
GO:0045087 innate immune response
GO:0045730 respiratory burst
GO:0046982 protein heterodimerization activity
GO:0050665 hydrogen peroxide biosynthetic process
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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