Cytochrome B245 antibody | CS9
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F ResetMouse anti Human Cytochrome B245 Light Chain
- Product Type
- Monoclonal Antibody
- Clone
- CS9
- Isotype
- IgG1
- Specificity
- Cytochrome B245
- Region
- LIGHT CHAIN
Antibody Quality and Performance Guarantee
| Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 recognizes the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. The light chain (p22phox), along with the heavy chain (gp91phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. The core region of the CS9 epitope is amino acids 165 - 169 (KKPSE), a cytosolic epitope on p22phox not accessible on intact neutrophils. Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 also has an inhibitory effect on NADPH oxidase activation. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Carrier Free
- Yes
- Immunogen
- Cytochrome B245 solubilised in octyl-beta-gluycopyranoside.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml.
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the 3PU1 myeloma cell line.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry 1 | |
1/10 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
||
| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Western Blotting
- Mouse anti Human cytochrome B245 antibody, clone CS9 detects a band of approximately 19kDa in neutrophil membrane extracts.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for Cytochrome B245 antibody
-
Taylor, R.M. et al. (2004) Site-specific inhibitors of NADPH oxidase activity and structural probes of flavocytochrome b: characterization of six monoclonal antibodies to the p22phox subunit.
J Immunol. 173 (12): 7349-57.
- Synonyms
- p22phox
- RRID
- AB_1935435
- UniProt
- P13498
- Entrez Gene
- CYBA
- GO Terms
- GO:0009055 electron carrier activity
- GO:0030141 stored secretory granule
- GO:0022900 electron transport chain
- GO:0006810 transport
- GO:0006954 inflammatory response
- GO:0014895 smooth muscle hypertrophy
- GO:0017004 cytochrome complex assembly
- GO:0017124 SH3 domain binding
- GO:0020037 heme binding
- View More GO Terms
- GO:0042554 superoxide anion generation
- GO:0043020 NADPH oxidase complex
- GO:0045087 innate immune response
- GO:0045730 respiratory burst
- GO:0046982 protein heterodimerization activity
- GO:0050665 hydrogen peroxide biosynthetic process
MCA4686
154812
MCA4686GA
152048
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