CD91 antibody | A2Mr alpha-2
Mouse anti Human CD91 antibody, clone A2Mr alpha-2 recognizes human CD91, also known as Prolow-density lipoprotein receptor-related protein 1, Alpha-2-macroglobulin receptor or apolipoprotein E receptor. CD91 is a 4525 amino acid protein post translationally cleaved into 3 subunits, a 85 kDa type I transmembrane carboxyl chain (LRP85) non-covalently bound to a 515 kDa extracellular N-terminal subunit (LRP515)containing multiple EGF-like and LDL-receptor Class A and Class B domains. Additionally, there is an intracellular domain (LRPICD) which can be cleaved from the transmambrane domain by gamma secretase (May et al. 2004). Clone A2Mr alpha-2 detects an epitope within the LRP515 chain.
CD91 is a multifunctional protein involved in processes inluding the phagocytosis and endocytosis of apoptotic cells (Nilsson et al. 2012), clearance of activated serum alpha-2-macroglobulin (Kristensen et al. 1990), modulation of the inflammatory response (Staudt et al. 2013) and acts as a receptor for Pseudomonas aeruginosa exotoxin A (Kounnas et al. 1992).
Mouse anti Human CD91, clone A2Mr alpha-2 has been used extensively for the detection of CD91 by flow cytometry and immunohistochemistry on formalin fixed paraffin embedded tissues (Bourazopoulou et al. 2009).
CD91 is a multifunctional protein involved in processes inluding the phagocytosis and endocytosis of apoptotic cells (Nilsson et al. 2012), clearance of activated serum alpha-2-macroglobulin (Kristensen et al. 1990), modulation of the inflammatory response (Staudt et al. 2013) and acts as a receptor for Pseudomonas aeruginosa exotoxin A (Kounnas et al. 1992).
Mouse anti Human CD91, clone A2Mr alpha-2 has been used extensively for the detection of CD91 by flow cytometry and immunohistochemistry on formalin fixed paraffin embedded tissues (Bourazopoulou et al. 2009).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Purified alpha2 macroglobulin receptor
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the NS1 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q07954
- Entrez Gene
- LRP1
- GO Terms
- GO:0005509 calcium ion binding
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005634 nucleus
- GO:0005905 coated pit
- GO:0007275 multicellular organismal development
- GO:0010875 positive regulation of cholesterol efflux
- GO:0030178 negative regulation of Wnt receptor signaling pathway
- GO:0032403 protein complex binding
- GO:0032429 regulation of phospholipase A2 activity
- GO:0034187 apolipoprotein E binding
- GO:0042954 lipoprotein transporter activity
- GO:0043277 apoptotic cell clearance
- GO:0070325 lipoprotein particle receptor binding
- Regulatory
- For research purposes only
Applications of CD91 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | Direct | ||
| Flow Cytometry | 1/50 1/100 | ||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting 1 | Non-reducing conditions | ||
| Flow Cytometry | Neat |
- 1A2Mr alpha-2 recognizes CD91 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Liver
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD91 antibody
-
Moestrup, S.K. et al. (1992) Distribution of the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein in human tissues.
Cell Tissue Res. 269 (3): 375-82. -
Moestrup, S.K. & Hokland, P. (1992) Surface expression of the alpha 2-macroglobulin receptor on human malignant blood cells.
Leuk Res. 16 (3): 227-34. -
Chen, J.S. et al. (2010) Secreted Heat Shock Protein 90{alpha} Induces Colorectal Cancer Cell Invasion through CD91/LRP-1 and NF-{kappa}B-mediated Integrin {alpha}V Expression.
J Biol Chem. 285: 25458-66. -
Bourazopoulou, E. et al. (2009) Functional expression of the alpha 2-macroglobulin receptor CD91 in salivary gland epithelial cells.
J Autoimmun. 33: 141-6. -
Hvidberg, V. et al. (2005) Identification of the receptor scavenging hemopexin-heme complexes.
Blood. 106: 2572-9. -
Hodge, S. et al. (2006) Azithromycin increases phagocytosis of apoptotic bronchial epithelial cells by alveolar macrophages.
Eur Respir J. 28 (3): 486-95. -
Hodge, S. et al. (2007) Smoking alters alveolar macrophage recognition and phagocytic ability: implications in chronic obstructive pulmonary disease.
Am J Respir Cell Mol Biol. 37: 748-55. -
Pawluczyk, I.Z. et al. (2008) Perindoprilat modulates the activity of lipoprotein receptor-related protein in human mesangial cells.
J Biol Chem. 283: 4588-94. -
Cunnington, A.J. et al. (2012) Prolonged neutrophil dysfunction after Plasmodium falciparum malaria is related to hemolysis and heme oxygenase-1 induction.
J Immunol. 189 (11): 5336-46. -
Inage, E. et al. (2014) Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2.
J Immunol. 192 (8): 3936-46.
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