CD91 antibody | A2Mr alpha-2

Mouse anti Human CD91

Product Type
Monoclonal Antibody
Clone
A2Mr alpha-2
Isotype
IgG1
Specificity
CD91

Product Code Applications Pack Size List Price Your Price Qty
MCA1965
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IF IP WB * 0.2 mg loader
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loader
MCA1965GA
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SDS Safety Datasheet SDS
C E F IP WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD91 antibody, clone A2Mr alpha-2 recognizes human CD91, also known as Prolow-density lipoprotein receptor-related protein 1, Alpha-2-macroglobulin receptor or apolipoprotein E receptor. CD91 is a 4525 amino acid protein post translationally cleaved into 3 subunits, a 85 kDa type I transmembrane carboxyl chain (LRP85) non-covalently bound to a 515 kDa extracellular N-terminal subunit (LRP515)containing multiple EGF-like and LDL-receptor Class A and Class B domains. Additionally, there is an intracellular domain (LRPICD) which can be cleaved from the transmambrane domain by gamma secretase (May et al. 2004). Clone A2Mr alpha-2 detects an epitope within the LRP515 chain.

CD91 is a multifunctional protein involved in processes inluding the phagocytosis and endocytosis of apoptotic cells (Nilsson et al. 2012), clearance of activated serum alpha-2-macroglobulin (Kristensen et al. 1990), modulation of the inflammatory response (Staudt et al. 2013) and acts as a receptor for Pseudomonas aeruginosa exotoxin A (Kounnas et al. 1992).

Mouse anti Human CD91, clone A2Mr alpha-2 has been used extensively for the detection of CD91 by flow cytometry and immunohistochemistry on formalin fixed paraffin embedded tissues (Bourazopoulou et al. 2009).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Purified alpha2 macroglobulin receptor
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Direct
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 1 Non-reducing conditions
  1. 1A2Mr alpha-2 recognizes CD91 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Liver

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD91 antibody

  1. Moestrup, S.K. et al. (1992) Distribution of the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein in human tissues.
    Cell Tissue Res. 269 (3): 375-82.
  2. Moestrup, S.K. & Hokland, P. (1992) Surface expression of the alpha 2-macroglobulin receptor on human malignant blood cells.
    Leuk Res. 16 (3): 227-34.
  3. Chen, J.S. et al. (2010) Secreted Heat Shock Protein 90{alpha} Induces Colorectal Cancer Cell Invasion through CD91/LRP-1 and NF-{kappa}B-mediated Integrin {alpha}V Expression.
    J Biol Chem. 285: 25458-66.
  4. Bourazopoulou, E. et al. (2009) Functional expression of the alpha 2-macroglobulin receptor CD91 in salivary gland epithelial cells.
    J Autoimmun. 33: 141-6.
  5. Hvidberg, V. et al. (2005) Identification of the receptor scavenging hemopexin-heme complexes.
    Blood. 106: 2572-9.
  6. Hodge, S. et al. (2006) Azithromycin increases phagocytosis of apoptotic bronchial epithelial cells by alveolar macrophages.
    Eur Respir J. 28 (3): 486-95.
  7. Hodge, S. et al. (2007) Smoking alters alveolar macrophage recognition and phagocytic ability: implications in chronic obstructive pulmonary disease.
    Am J Respir Cell Mol Biol. 37: 748-55.
  8. Pawluczyk, I.Z. et al. (2008) Perindoprilat modulates the activity of lipoprotein receptor-related protein in human mesangial cells.
    J Biol Chem. 283: 4588-94.
  9. View The Latest Product References
  10. Cunnington, A.J. et al. (2012) Prolonged neutrophil dysfunction after Plasmodium falciparum malaria is related to hemolysis and heme oxygenase-1 induction.
    J Immunol. 189 (11): 5336-46.
  11. Inage, E. et al. (2014) Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2.
    J Immunol. 192 (8): 3936-46.

Immunofluorescence

Western Blotting

Synonyms
ALPHA-2 Macroglobulin Receptor
RRID
AB_323265
UniProt
Q07954
Entrez Gene
LRP1
GO Terms
GO:0005509 calcium ion binding
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005634 nucleus
GO:0005905 coated pit
GO:0007275 multicellular organismal development
GO:0010875 positive regulation of cholesterol efflux
GO:0030178 negative regulation of Wnt receptor signaling pathway
GO:0032403 protein complex binding
GO:0032429 regulation of phospholipase A2 activity
GO:0034187 apolipoprotein E binding
GO:0042954 lipoprotein transporter activity
GO:0043277 apoptotic cell clearance
GO:0070325 lipoprotein particle receptor binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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