CD91 antibody | A2Mr alpha-2
Mouse anti Human CD91 antibody, clone A2Mr alpha-2 recognizes human CD91, also known as Prolow-density lipoprotein receptor-related protein 1, Alpha-2-macroglobulin receptor or apolipoprotein E receptor. CD91 is a 4525 amino acid protein post translationally cleaved into 3 subunits, a 85 kDa type I transmembrane carboxyl chain (LRP85) non-covalently bound to a 515 kDa extracellular N-terminal subunit (LRP515)containing multiple EGF-like and LDL-receptor Class A and Class B domains. Additionally, there is an intracellular domain (LRPICD) which can be cleaved from the transmambrane domain by gamma secretase (May et al. 2004). Clone A2Mr alpha-2 detects an epitope within the LRP515 chain.
CD91 is a multifunctional protein involved in processes inluding the phagocytosis and endocytosis of apoptotic cells (Nilsson et al. 2012), clearance of activated serum alpha-2-macroglobulin (Kristensen et al. 1990), modulation of the inflammatory response (Staudt et al. 2013) and acts as a receptor for Pseudomonas aeruginosa exotoxin A (Kounnas et al. 1992).
Mouse anti Human CD91, clone A2Mr alpha-2 has been used extensively for the detection of CD91 by flow cytometry and immunohistochemistry on formalin fixed paraffin embedded tissues (Bourazopoulou et al. 2009).
CD91 is a multifunctional protein involved in processes inluding the phagocytosis and endocytosis of apoptotic cells (Nilsson et al. 2012), clearance of activated serum alpha-2-macroglobulin (Kristensen et al. 1990), modulation of the inflammatory response (Staudt et al. 2013) and acts as a receptor for Pseudomonas aeruginosa exotoxin A (Kounnas et al. 1992).
Mouse anti Human CD91, clone A2Mr alpha-2 has been used extensively for the detection of CD91 by flow cytometry and immunohistochemistry on formalin fixed paraffin embedded tissues (Bourazopoulou et al. 2009).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Purified alpha2 macroglobulin receptor
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the NS1 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- Q07954
- Entrez Gene
- LRP1
- GO Terms
- GO:0005509 calcium ion binding
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005634 nucleus
- GO:0005905 coated pit
- GO:0007275 multicellular organismal development
- GO:0010875 positive regulation of cholesterol efflux
- GO:0030178 negative regulation of Wnt receptor signaling pathway
- GO:0032403 protein complex binding
- GO:0032429 regulation of phospholipase A2 activity
- GO:0034187 apolipoprotein E binding
- GO:0042954 lipoprotein transporter activity
- GO:0043277 apoptotic cell clearance
- GO:0070325 lipoprotein particle receptor binding
- Regulatory
- For research purposes only
Applications of CD91 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | Direct | ||
| Flow Cytometry | 1/50 1/100 | ||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting 1 | Non-reducing conditions | ||
| Flow Cytometry | Neat |
- 1A2Mr alpha-2 recognizes CD91 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Liver
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD91 antibody
-
Moestrup, S.K. et al. (1992) Distribution of the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein in human tissues.
Cell Tissue Res. 269 (3): 375-82. -
Moestrup, S.K. & Hokland, P. (1992) Surface expression of the alpha 2-macroglobulin receptor on human malignant blood cells.
Leuk Res. 16 (3): 227-34. -
Chen, J.S. et al. (2010) Secreted Heat Shock Protein 90{alpha} Induces Colorectal Cancer Cell Invasion through CD91/LRP-1 and NF-{kappa}B-mediated Integrin {alpha}V Expression.
J Biol Chem. 285: 25458-66. -
Bourazopoulou, E. et al. (2009) Functional expression of the alpha 2-macroglobulin receptor CD91 in salivary gland epithelial cells.
J Autoimmun. 33: 141-6. -
Hvidberg, V. et al. (2005) Identification of the receptor scavenging hemopexin-heme complexes.
Blood. 106: 2572-9. -
Hodge, S. et al. (2006) Azithromycin increases phagocytosis of apoptotic bronchial epithelial cells by alveolar macrophages.
Eur Respir J. 28 (3): 486-95. -
Hodge, S. et al. (2007) Smoking alters alveolar macrophage recognition and phagocytic ability: implications in chronic obstructive pulmonary disease.
Am J Respir Cell Mol Biol. 37: 748-55. -
Pawluczyk, I.Z. et al. (2008) Perindoprilat modulates the activity of lipoprotein receptor-related protein in human mesangial cells.
J Biol Chem. 283: 4588-94. -
Cunnington, A.J. et al. (2012) Prolonged neutrophil dysfunction after Plasmodium falciparum malaria is related to hemolysis and heme oxygenase-1 induction.
J Immunol. 189 (11): 5336-46. -
Inage, E. et al. (2014) Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2.
J Immunol. 192 (8): 3936-46.
