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CD91 antibody | A2Mr alpha-2

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Anti Human CD91 Antibody, clone A2Mr alpha-2 thumbnail image 1 Anti Human CD91 Antibody, clone A2Mr alpha-2 thumbnail image 2 Anti Human CD91 Antibody, clone A2Mr alpha-2 thumbnail image 3 Anti Human CD91 Antibody, clone A2Mr alpha-2 thumbnail image 4 Anti Human CD91 Antibody, clone A2Mr alpha-2 thumbnail image 5 Anti Human CD91 Antibody, clone A2Mr alpha-2 thumbnail image 6 Anti Human CD91 Antibody, clone A2Mr alpha-2 thumbnail image 7
Anti Human CD91 Antibody, clone A2Mr alpha-2 gallery image 1
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 (MCA1965) followed by the Histar detection system (STAR3000). Low power.
Anti Human CD91 Antibody, clone A2Mr alpha-2 gallery image 2
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 (MCA1965) followed by the Histar detection system (STAR3000). Medium power.
Anti Human CD91 Antibody, clone A2Mr alpha-2 gallery image 3
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 (MCA1965) followed by the Histar detection system (STAR3000). Medium power.
Anti Human CD91 Antibody, clone A2Mr alpha-2 gallery image 4
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 (MCA1965) followed by the Histar detection system (STAR3000). High power.
Anti Human CD91 Antibody, clone A2Mr alpha-2 gallery image 5
Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 (MCA1965), red in A and Mouse anti Human CD21, clone LB21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power.
Anti Human CD91 Antibody, clone A2Mr alpha-2 gallery image 6
Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 (MCA1965), red in A and Mouse anti Human CD21, clone LB21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power
Anti Human CD91 Antibody, clone A2Mr alpha-2 gallery image 7
Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 (MCA1965), red in A and Mouse anti Human CD21, clone LB21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. High power

Mouse anti Human CD91

Mouse anti Human CD91:RPE

Product Type
Monoclonal Antibody
Clone
A2Mr alpha-2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1965
Datasheet
C E F IF IP WB*
SDS
0.2 mg loader

MCA1965GA
Datasheet
C E F IP WB
SDS
0.1 mg loader

MCA1965PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Human CD91 antibody, clone A2Mr alpha-2 recognizes human CD91, also known as Prolow-density lipoprotein receptor-related protein 1, Alpha-2-macroglobulin receptor or apolipoprotein E receptor. CD91 is a 4525 amino acid protein post translationally cleaved into 3 subunits, a 85 kDa type I transmembrane carboxyl chain (LRP85) non-covalently bound to a 515 kDa extracellular N-terminal subunit (LRP515)containing multiple EGF-like and LDL-receptor Class A and Class B domains. Additionally, there is an intracellular domain (LRPICD) which can be cleaved from the transmambrane domain by gamma secretase (May et al. 2004). Clone A2Mr alpha-2 detects an epitope within the LRP515 chain.

CD91 is a multifunctional protein involved in processes inluding the phagocytosis and endocytosis of apoptotic cells (Nilsson et al. 2012), clearance of activated serum alpha-2-macroglobulin (Kristensen et al. 1990), modulation of the inflammatory response (Staudt et al. 2013) and acts as a receptor for Pseudomonas aeruginosa exotoxin A (Kounnas et al. 1992).

Mouse anti Human CD91, clone A2Mr alpha-2 has been used extensively for the detection of CD91 by flow cytometry and immunohistochemistry on formalin fixed paraffin embedded tissues (Bourazopoulou et al. 2009).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Purified alpha2 macroglobulin receptor
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q07954
Entrez Gene
LRP1
GO Terms
GO:0005509 calcium ion binding
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005634 nucleus
GO:0005905 coated pit
GO:0007275 multicellular organismal development
GO:0010875 positive regulation of cholesterol efflux
GO:0030178 negative regulation of Wnt receptor signaling pathway
GO:0032403 protein complex binding
GO:0032429 regulation of phospholipase A2 activity
GO:0034187 apolipoprotein E binding
GO:0042954 lipoprotein transporter activity
GO:0043277 apoptotic cell clearance
GO:0070325 lipoprotein particle receptor binding
Regulatory
For research purposes only

Applications of CD91 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Direct
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 1 Non-reducing conditions
Flow Cytometry Neat
  1. 1A2Mr alpha-2 recognizes CD91 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Liver
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for CD91 antibody

  1. Moestrup, S.K. et al. (1992) Distribution of the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein in human tissues.
    Cell Tissue Res. 269 (3): 375-82.
  2. Moestrup, S.K. & Hokland, P. (1992) Surface expression of the alpha 2-macroglobulin receptor on human malignant blood cells.
    Leuk Res. 16 (3): 227-34.
  3. Chen, J.S. et al. (2010) Secreted Heat Shock Protein 90{alpha} Induces Colorectal Cancer Cell Invasion through CD91/LRP-1 and NF-{kappa}B-mediated Integrin {alpha}V Expression.
    J Biol Chem. 285: 25458-66.
  4. Bourazopoulou, E. et al. (2009) Functional expression of the alpha 2-macroglobulin receptor CD91 in salivary gland epithelial cells.
    J Autoimmun. 33: 141-6.
  5. Hvidberg, V. et al. (2005) Identification of the receptor scavenging hemopexin-heme complexes.
    Blood. 106: 2572-9.
  6. Hodge, S. et al. (2006) Azithromycin increases phagocytosis of apoptotic bronchial epithelial cells by alveolar macrophages.
    Eur Respir J. 28 (3): 486-95.
  7. Hodge, S. et al. (2007) Smoking alters alveolar macrophage recognition and phagocytic ability: implications in chronic obstructive pulmonary disease.
    Am J Respir Cell Mol Biol. 37: 748-55.
  8. Pawluczyk, I.Z. et al. (2008) Perindoprilat modulates the activity of lipoprotein receptor-related protein in human mesangial cells.
    J Biol Chem. 283: 4588-94.
  9. Cunnington, A.J. et al. (2012) Prolonged neutrophil dysfunction after Plasmodium falciparum malaria is related to hemolysis and heme oxygenase-1 induction.
    J Immunol. 189 (11): 5336-46.
  10. Inage, E. et al. (2014) Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2.
    J Immunol. 192 (8): 3936-46.

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