CD21 Antibody | LB21

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CD21 Antibody | LB21 gallery image 1

Staining of human peripheral blood lymphocytes with Mouse anti Human CD21 (MCA1195)

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CD21 Antibody | LB21 gallery image 2

Staining of human peripheral blood lymphocytes with Mouse anti Human CD21:FITC (MCA1195F)

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CD21 Antibody | LB21 gallery image 3

Staining of human peripheral blood lymphocytes with Mouse anti Human CD21:RPE (MCA1195PE)

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CD21 Antibody | LB21 gallery image 4

Immunofluorescence image of human tonsil cryosection stained with Sheep anti Human LOX-1 antibody (AHP952) in red and with Mouse anti Human CD21 antibody (MCA1195) in red. Nuclei are stained blue using DAPI. Merged image on right

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CD21 Antibody | LB21 gallery image 5

Immunofluorescence staining of human tonsil cryosection using Mouse anti Human CD1a antibody (MCA80), red in A and Mouse anti Human CD21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. High power

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CD21 Antibody | LB21 gallery image 6

Immunoperoxidase staining of human spleen cryosection using Mouse ati Human CD21 antibody (MCA1195) followed by HISTAR detection system. Low power

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CD21 Antibody | LB21 gallery image 7

Immunoperoxidase staining of human spleen cryosection using Mouse ati Human CD21 antibody (MCA1195) followed by HISTAR detection system. High power

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CD21 Antibody | LB21 gallery image 8

Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody (MCA1195) followed by HISTAR detection system. Medium power

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CD21 Antibody | LB21 gallery image 9

Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody (MCA1195) followed by HISTAR detection system. Medium power

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CD21 Antibody | LB21 gallery image 10

Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody (MCA1195) followed by HISTAR detection system. High power

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CD21 Antibody | LB21 gallery image 11

Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 (MCA551), red in A and Mouse anti Human CD21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. medium power

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CD21 Antibody | LB21 gallery image 12

Immunofluorescence staining of a human tonsil cryostection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582), red in A and Mouse anti Human CD21 antibody, clone LB21 (MCA1195), green in B. The merged image is in C with nuclei counterstained blue using DAPI. Low power

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CD21 Antibody | LB21 gallery image 13

Immunofluorescence staining of a human tonsil cryostection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582), red in A and Mouse anti Human CD21 antibody, clone LB21 (MCA1195), green in B. The merged image is in C with nuclei counterstained blue using DAPI. High power

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  • Mouse anti Human CD21:FITC
  • Mouse anti Human CD21:FITC
  • Mouse anti Human CD21:RPE
  • Mouse anti Human CD21
  • Mouse anti Human CD21:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    LB21
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1195FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1195F
    MCA1195PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1195PE
    MCA1195C *, F, IPdatasheet pdfdatasheet pdf2 ml
    MCA1195
    MCA1195FTFdatasheet pdfdatasheet pdf25 µg
    MCA1195FT
    MCA1195PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1195PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD21 antibody, clone LB21 recognizes the human Complement receptor type 2, also known as CD21 or the Epstein-Barr virus receptor. CD21 is a ~14 kDa cell surface glycoprotein expressed by mature B cells and by follicular dendritic cells. The molecule acts as a receptor for complement components C3d, C3dg and iC3b, as well as for Epstein Barr Virus. It forms part of a large signal transduction complex in association with CD19.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Sheepyes
      Minkyes
      Bovineyes
      Goatyes
      Catyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by ion exchange chromotography
      Purified IgG prepared by ion exchange chromotography
      Purified IgG prepared by ion exchange chromatography
      Purified IgG prepared by ion exchange chromatography
      Purified IgG prepared by ion exchange chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Human IM9 cell line.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 25 ug/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recomend microcentrifuging before use
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recomend microcentrifuging before use
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      complement activation, classical pathway
      plasma membrane
      integral to membrane
      complement receptor activity
      innate immune response
      protein homodimerization activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/101/100
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul human whole blood.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Lymph node
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD21 Antibody Formats

    Formats Clone Applications Sizes available
    CD21 Antibody : RPE LB21 F 25 Tests | 100 Tests
    CD21 Antibody : Purified LB21 C *, F, IP 2 ml
    CD21 Antibody : FITC LB21 F 0.1 mg | 25 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
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      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
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      STAR117F
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      STAR70
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      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
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      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
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      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA928PE100 TestsF
        MCA928PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        MCA928PE

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              Lymph node
              • Application NameReference Images
                Flow Cytometry
                Immunofluorescence

              References

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