CD9 antibody | MM2/57

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MCA469G
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SDS Safety Datasheet SDS
C F WB 0.2 mg loader
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loader
MCA469GA
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SDS Safety Datasheet SDS
C F WB 0.1 mg loader
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loader
MCA469GT
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C F WB 25 µg loader
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Mouse anti Human CD9 antibody, clone MM2/57 recognizes human leukocyte antigen MIC3 also known as MRP-1 or CD9. CD9 is a 228 amino acid multi pass membrane glycoprotein belonging to the tetraspanin family with a molecular weight of ~24 kDa expressed by platelets, monocytes, some lymphocytes and endothelial cells.

Mouse anti Human CD9 antibody, clone MM2/57 recognizes a conserved epitope on CD9 present on a wide range of mammalian species.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
Rhesus Monkey
Bovine
Dog
Rabbit
Horse
Pig
Mustelid Expected from Sequence
Mink
Llama
Ferret
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Human platelet membranes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells from the SP2/0 mouse myeloma line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunohistology - Frozen 1/500 1/1000
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

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References for CD9 antibody

  1. Ed Knapp W. et al. (1989) Leucocyte Typing IV
    Oxford University Press.
  2. Boucheix, C. et al. (1991) Molecular cloning of the CD9 antigen. A new family of cell surface proteins.
    J Biol Chem. 266 (1): 117-22.
  3. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
    Vet Immunol Immunopathol. 64 (1): 1-13.
  4. Ibrahim,S. et al. (2007) Screening of anti-human leukocyte monoclonal antibodies for reactivity with equine leukocytes
    Vet.Immunol Immunopathol. 119: 63-80
  5. Jennings, L. K. et al. (1995) CD9 cluster workshop report: cell surface binding and functional analysis.
    In S.F. Sclossman. et al. Editors. 1995. Leucocyte Typing V. White Cell Differentiation Antigens. Oxford University Press, New York, NY. 1249-1251.
  6. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
    Vet Immunol Immunopathol. 132:109-15.
  7. Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
    Vet Immunol Immunopathol. 119: 27-37.
  8. Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
    Vet Immunol Immunopathol. 119: 123-30.
  9. View The Latest Product References
  10. Ferrer, M. et al. (1998) Pattern of expression of tetraspanin antigen genes in Burkitt lymphoma cell lines.
    Clin Exp Immunol. 113: 346-52.
  11. Kao, Y.R. et al. (2003) Tumor-associated antigen L6 and the invasion of human lung cancer cells.
    Clin Cancer Res. 9: 2807-16.
  12. Müller, T. et al. (2009) A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics.
    Hum Reprod. 24: 1359-72.
  13. Kubota, H. et al. (2011) Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
    FASEB J. 25 (8): 2604-14.
  14. Hogue, I.B. et al. (2011) Gag induces the coalescence of clustered lipid rafts and tetraspanin-enriched microdomains at HIV-1 assembly sites on the plasma membrane.
    J Virol. 85 (19): 9749-66.
  15. Löffler, S. et al. (1997) CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus.
    J Virol. 71: 42-9.
  16. Meister, R.K. et al. (2007) Progress in the discovery and definition of monoclonal antibodies for use in feline research.
    Vet Immunol Immunopathol. 119: 38-46.
  17. Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
    Stem Cell Res Ther. 8 (1): 218.
  18. Jackson, C.E. et al. (2017) Effects of Inhibiting VPS4 Support a General Role for ESCRTs in Extracellular Vesicle Biogenesis.
    Biophys J. 113 (6): 1342-1352.
  19. Wąchalska, M. et al. (2020) Palmitoylated mNeonGreen Protein as a Tool for Visualization and Uptake Studies of Extracellular Vesicles
    Membranes. 10 (12): 373.
  20. Fish, E.J. et al. (2018) Malignant canine mammary epithelial cells shed exosomes containing differentially expressed microRNA that regulate oncogenic networks.
    BMC Cancer. 18 (1): 832.
  21. Fu, T. et al. (2021) Biomimetic vascularized adipose-derived mesenchymal stem cells bone-periosteum graft enhances angiogenesis and osteogenesis in a rabbit spine fusion model.
    Stem Cell Res Therapy.15 Sep [Epub ahead of print].
  22. Viswanathan, K. et al. (2017) Quantitative membrane proteomics reveals a role for tetraspanin enriched microdomains during entry of human cytomegalovirus.
    PLoS One. 12 (11): e0187899.

Flow Cytometry

Synonyms
MRP-1
RRID
AB_323961
UniProt
P21926
Entrez Gene
CD9
GO Terms
GO:0005515 protein binding
GO:0002576 platelet degranulation
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0006928 cellular component movement
GO:0007342 fusion of sperm to egg plasma membrane
GO:0030168 platelet activation
GO:0030913 paranodal junction assembly
GO:0031092 platelet alpha granule membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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