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CD9 antibody | MM2/57

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Anti Human CD9 Antibody, clone MM2/57 thumbnail image 1 Anti Human CD9 Antibody, clone MM2/57 thumbnail image 2 Anti Human CD9 Antibody, clone MM2/57 thumbnail image 3
Anti Human CD9 Antibody, clone MM2/57 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD14 (MCA596F) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. FITC conjugated Mouse anti Human CD14 (MCA596F) and RPE conjugated Mouse anti Human CD9 (MCA469PE). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A)
Anti Human CD9 Antibody, clone MM2/57 gallery image 2
Staining of acetone fixed frozen human tonsil with Mouse anti Human CD9 (MCA469GA)
Anti Human CD9 Antibody, clone MM2/57 gallery image 3
Published customer image:
Mouse anti Human CD9 antibody, clone MM2/57 (MCA469GA) used as part of a panel of antibodies to demonstrate the phenotype of canine mesenchymal stem cells by flow cytometry together with mesenchymal markers CD44, CD90 and CD105. Negative results for lineage cell markers CD34, CD45 and STRO-1 are shown.
Image caption:
b. Representative histograms demonstrating positive and negative staining of marrow cMSCs from a single donor

From: Bearden RN, Huggins SS, Cummings KJ, Smith R, Gregory CA, Saunders WB.
In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
Stem Cell Res Ther. 2017 Oct 3;8(1):218.

Mouse anti Human CD9:Alexa Fluor® 488

Mouse anti Human CD9:Alexa Fluor® 647

Mouse anti Human CD9:Biotin

Mouse anti Human CD9:FITC

Mouse anti Human CD9:Pacific Blue®

Mouse anti Human CD9

Mouse anti Human CD9:RPE

Product Type
Monoclonal Antibody
Clone
MM2/57
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA469A488 F 100 Tests/1ml
MCA469A647 F 100 Tests/1ml
MCA469A647T F 25 Tests/0.25ml
MCA469B F 0.1 mg
MCA469BT F 25 µg
MCA469F F 0.1 mg
MCA469FT F 25 µg
MCA469PB F 100 Tests/1ml
MCA469G C F WB 0.2 mg
MCA469GA C F WB 0.1 mg
MCA469GT C F WB 25 µg
MCA469PE F 100 Tests
MCA469PET F 25 Tests
Mouse anti Human CD9 antibody, clone MM2/57 recognizes human leukocyte antigen MIC3 also known as MRP-1 or CD9. CD9 is a 228 amino acid multi pass membrane glycoprotein belonging to the tetraspanin family with a molecular weight of ~24 kDa expressed by platelets, monocytes, some lymphocytes and endothelial cells.

Mouse anti Human CD9 antibody, clone MM2/57 recognizes a conserved epitope on CD9 present on a wide range of mammalian species.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
Rhesus Monkey
Bovine
Dog
Rabbit
Horse
Pig
Mustelid Expected from Sequence
Mink
Llama
Ferret
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Biotin - liquid.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Pacific Blue - liquid
Product Form
Purified IgG - liquid
Product Form
Pack Size: 100 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Pack Size: 25 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Reconstitution
Reconstitute with 1ml distilled water.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Human platelet membranes
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells from the SP2/0 mouse myeloma line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 25 Tests
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P21926 Related reagents
Entrez Gene
CD9 Related reagents
GO Terms
GO:0005515 protein binding
GO:0002576 platelet degranulation
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0006928 cellular component movement
GO:0007342 fusion of sperm to egg plasma membrane
GO:0030168 platelet activation
GO:0030913 paranodal junction assembly
GO:0031092 platelet alpha granule membrane
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/5
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry 1/100 1/200
Immunohistology - Frozen 1/500 1/1000
Western Blotting
Flow Cytometry Neat 1/2
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2b Negative Control:Alexa Fluor® 488 MCA691A488 100 Tests/1ml F
Mouse IgG2b Negative Control:Alexa Fluor® 647 MCA691A647 100 Tests/1ml F
Mouse IgG2b Negative Control:FITC MCA691F 100 Tests F
Mouse IgG2b Negative Control:Pacific Blue® MCA691PB 100 Tests/1ml F
Mouse IgG2b Negative Control MCA691 100 Tests F
Mouse IgG2b Negative Control:RPE MCA691PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD9 antibody

  1. Boucheix, C. et al. (1991) Molecular cloning of the CD9 antigen. A new family of cell surface proteins.
    J Biol Chem. 266 (1): 117-22.
  2. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
    Vet Immunol Immunopathol. 64 (1): 1-13.
  3. Ibrahim,S. et al. (2007) Screening of anti-human leukocyte monoclonal antibodies for reactivity with equine leukocytes
    Vet.Immunol Immunopathol. 119: 63-80
  4. Jennings, L. K. et al. (1995) CD9 cluster workshop report: cell surface binding and functional analysis.
    In S.F. Sclossman. et al. Editors. 1995. Leucocyte Typing V. White Cell Differentiation Antigens. Oxford University Press, New York, NY. 1249-1251.
  5. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
    Vet Immunol Immunopathol. 132:109-15.
  6. Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
    Vet Immunol Immunopathol. 119: 27-37.
  7. Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
    Vet Immunol Immunopathol. 119: 123-30.
  8. Ferrer, M. et al. (1998) Pattern of expression of tetraspanin antigen genes in Burkitt lymphoma cell lines.
    Clin Exp Immunol. 113: 346-52.
  9. Kao, Y.R. et al. (2003) Tumor-associated antigen L6 and the invasion of human lung cancer cells.
    Clin Cancer Res. 9: 2807-16.
  10. Müller, T. et al. (2009) A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics.
    Hum Reprod. 24: 1359-72.
  11. Kubota, H. et al. (2011) Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
    FASEB J. 25 (8): 2604-14.
  12. Hogue, I.B. et al. (2011) Gag induces the coalescence of clustered lipid rafts and tetraspanin-enriched microdomains at HIV-1 assembly sites on the plasma membrane.
    J Virol. 85 (19): 9749-66.
  13. Löffler, S. et al. (1997) CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus.
    J Virol. 71: 42-9.
  14. Meister, R.K. et al. (2007) Progress in the discovery and definition of monoclonal antibodies for use in feline research.
    Vet Immunol Immunopathol. 119: 38-46.
  15. Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
    Stem Cell Res Ther. 8 (1): 218.
  16. Jackson, C.E. et al. (2017) Effects of Inhibiting VPS4 Support a General Role for ESCRTs in Extracellular Vesicle Biogenesis.
    Biophys J. 113 (6): 1342-1352.
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