CD9 antibody | MM2/57
Mouse anti Human CD9 antibody, clone MM2/57 (MCA469GA) used as part of a panel of antibodies to demonstrate the phenotype of canine mesenchymal stem cells by flow cytometry together with mesenchymal markers CD44, CD90 and CD105. Negative results for lineage cell markers CD34, CD45 and STRO-1 are shown.
Image caption:
b. Representative histograms demonstrating positive and negative staining of marrow cMSCs from a single donor
From: Bearden RN, Huggins SS, Cummings KJ, Smith R, Gregory CA, Saunders WB.
In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
Stem Cell Res Ther. 2017 Oct 3;8(1):218.
Mouse anti Human CD9 antibody, clone MM2/57 (MCA649G) used for the evaluation of CD9 expression in canine cell line lysates by western blotting.
Image caption:
Insert image caption
From: Fish EJ, Irizarry KJ, DeInnocentes P, Ellis CJ, Prasad N, Moss AG, Curt Bird R.
Malignant canine mammary epithelial cells shed exosomes containing differentially expressed microRNA that regulate oncogenic networks.
BMC Cancer. 2018 Aug 20;18(1):832.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD9 antibody, clone MM2/57 (MCA469G) used for the evaluation of CD9 expression in lysates from human melanoma derived extracellular vesicles by immunoblotting.
Image caption:
Localization of palmNG in EVs. Various amounts of EVs from MJS or MJSpalmNG cells were dot-blotted in a dose range on a nitrocellulose membrane and analyzed by immunoblotting with the use of anti-mNeonGreen or anti-CD9 antibody in the presence or absence of a detergent.
From: Wąchalska, M. et al.
Palmitoylated mNeonGreen Protein as a Tool for Visualization and Uptake Studies of Extracellular Vesicles.
Membranes 2020, 1(12),373.
10.3390/membranes10120373..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD9 antibody, clone MM2/57 (MCA469G) used to evaluate exosome levels in virally infected MDCK cell lysates by western blotting.
Image caption:
Identification and characterization of exosomes.(c) Immunoblotting of exosomal marker (CD9) and IAV matrix protein (M1). Exosomes secreted by mock–infected (mock 1, 2) and IAV infected (flu 1, 2) cells; IAV–influenza virions; MDCK–mock–infected (−) and IAV infected cells (+).
From: Kwasnik M, Socha W, Czech B, Wasiak M, Rola J, Rozek W.
Protein-Coding Region Derived Small RNA in Exosomes from Influenza A Virus-Infected Cells.
Int J Mol Sci. 2023 Jan 3;24(1):867.
doi: 10.3390/ijms24010867.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F C WB IB ResetMouse anti Human CD9
- Product Type
- Monoclonal Antibody
- Clone
- MM2/57
- Isotype
- IgG2b
- Specificity
- CD9
Antibody Quality and Performance Guarantee
| Mouse anti Human CD9 antibody, clone MM2/57 recognizes human leukocyte antigen MIC3 also known as MRP-1 or CD9. CD9 is a 228 amino acid multi pass membrane glycoprotein belonging to the tetraspanin family with a molecular weight of ~24 kDa expressed by platelets, monocytes, some lymphocytes and endothelial cells. Mouse anti Human CD9 antibody, clone MM2/57 recognizes a conserved epitope on CD9 present on a wide range of mammalian species. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cat Rhesus Monkey Bovine Dog Rabbit Horse Pig Mustelid Expected from Sequence Mink Llama Ferret - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human platelet membranes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells from the SP2/0 mouse myeloma line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
| Immunohistology - Frozen | |
1/500 | 1/1000 |
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2b Negative Control | ||||||
References for CD9 antibody
-
Ed Knapp W. et al. (1989) Leucocyte Typing IV
Oxford University Press. -
Boucheix, C. et al. (1991) Molecular cloning of the CD9 antigen. A new family of cell surface proteins.
J Biol Chem. 266 (1): 117-22. -
Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
Vet Immunol Immunopathol. 64 (1): 1-13. -
Ibrahim,S. et al. (2007) Screening of anti-human leukocyte monoclonal antibodies for reactivity with equine leukocytes
Vet.Immunol Immunopathol. 119: 63-80 -
Jennings, L. K. et al. (1995) CD9 cluster workshop report: cell surface binding and functional analysis.
In S.F. Sclossman. et al. Editors. 1995. Leucocyte Typing V. White Cell Differentiation Antigens. Oxford University Press, New York, NY. 1249-1251. -
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. -
Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37. -
Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
Vet Immunol Immunopathol. 119: 123-30.
View The Latest Product References
-
Ferrer, M. et al. (1998) Pattern of expression of tetraspanin antigen genes in Burkitt lymphoma cell lines.
Clin Exp Immunol. 113: 346-52. -
Kao, Y.R. et al. (2003) Tumor-associated antigen L6 and the invasion of human lung cancer cells.
Clin Cancer Res. 9: 2807-16. -
Müller, T. et al. (2009) A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics.
Hum Reprod. 24: 1359-72. -
Kubota, H. et al. (2011) Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
FASEB J. 25 (8): 2604-14. -
Hogue, I.B. et al. (2011) Gag induces the coalescence of clustered lipid rafts and tetraspanin-enriched microdomains at HIV-1 assembly sites on the plasma membrane.
J Virol. 85 (19): 9749-66. -
Löffler, S. et al. (1997) CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus.
J Virol. 71: 42-9. -
Meister, R.K. et al. (2007) Progress in the discovery and definition of monoclonal antibodies for use in feline research.
Vet Immunol Immunopathol. 119: 38-46. -
Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
Stem Cell Res Ther. 8 (1): 218. -
Jackson, C.E. et al. (2017) Effects of Inhibiting VPS4 Support a General Role for ESCRTs in Extracellular Vesicle Biogenesis.
Biophys J. 113 (6): 1342-1352. -
Wąchalska, M. et al. (2020) Palmitoylated mNeonGreen Protein as a Tool for Visualization and Uptake Studies of Extracellular Vesicles
Membranes. 10 (12): 373. -
Fish, E.J. et al. (2018) Malignant canine mammary epithelial cells shed exosomes containing differentially expressed microRNA that regulate oncogenic networks.
BMC Cancer. 18 (1): 832. -
Fu, T. et al. (2021) Biomimetic vascularized adipose-derived mesenchymal stem cells bone-periosteum graft enhances angiogenesis and osteogenesis in a rabbit spine fusion model.
Stem Cell Res Therapy.15 Sep [Epub ahead of print]. -
Viswanathan, K. et al. (2017) Quantitative membrane proteomics reveals a role for tetraspanin enriched microdomains during entry of human cytomegalovirus.
PLoS One. 12 (11): e0187899. -
Kwasnik, M. et al. (2023) Protein-Coding Region Derived Small RNA in Exosomes from Influenza A Virus-Infected Cells.
Int J Mol Sci. 24 (1): 867.
- Synonyms
- MRP-1
- RRID
- AB_323961
- UniProt
- P21926
- Entrez Gene
- CD9
- GO Terms
- GO:0005515 protein binding
- GO:0002576 platelet degranulation
- GO:0007155 cell adhesion
- GO:0005887 integral to plasma membrane
- GO:0006928 cellular component movement
- GO:0007342 fusion of sperm to egg plasma membrane
- GO:0030168 platelet activation
- GO:0030913 paranodal junction assembly
- GO:0031092 platelet alpha granule membrane
- View More GO Terms
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