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CD74 antibody | Bu45

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Anti Human CD74 Antibody, clone Bu45 gallery image 1
Figure A. RPE-Alexa Fluor 750 tandem conjugated Mouse anti Human CD19 (MCA1940P750) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE-Alexa Fluor 750 tandem conjugated Mouse anti Human CD19 (MCA1940P750) and FITC conjugated Mouse anti Human CD74 (MCA2502F). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse anti Human CD74:FITC

Mouse anti Human CD74

Mouse anti Human CD74:RPE

Product Type
Monoclonal Antibody
Clone
Bu45
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2502F
Datasheet
F*
SDS
0.1 mg loader

MCA2502
Datasheet
C* F* FN* IF IP
SDS
0.2 mg loader

MCA2502PE
Datasheet
F*
SDS
100 Tests loader

Mouse anti Human CD74 antibody, clone Bu45 recognizes human CD74, a type II transmembrane protein, also known as the MHC class II associated invariant chain. The Bu45 antibody recognizes 33, 35 and 41 kDa isoforms of CD74. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes.

CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. Mouse anti Human CD74 antibody, clone Bu45 binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified Protein conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
B lymphoblastoid cell line HFB1.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the Ag8.653 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P04233
Entrez Gene
CD74
Regulatory
For research purposes only

Applications of CD74 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/5
Flow Cytometry 1 1/25 1/50
Functional Assays 2
Immunofluorescence
Immunohistology - Frozen 3
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilisation may enhance CD74 staining. Bio-Rad recommend the use of LeucopermTM (product code BUF09) for this purpose.
  1. 1 Membrane permeabilisation may enhance CD74 staining. Bio-Rad recommend the use of LeucopermTM (product code BUF09) for this purpose.
  2. 2 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
  3. 3The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  1. 1 Membrane permeabilization may enhance CD74 staining. Bio-Rad recommend the use of LeucopermTM (product code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Leucoperm BUF09 F 50 Tests
Leucoperm BUF09 F 50 Tests
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Leucoperm BUF09 F 50 Tests

Application Based External Images

Flow Cytometry

Functional Assays

Immunofluorescence

Immunoprecipitation

Product Specific References

References for CD74 antibody

  1. Wraight, C.J. et al. (1990) Human major histocompatibility complex class II invariant chain is expressed on the cell surface.
    J Biol Chem. 265 (10): 5787-92.
  2. Beswick, E.J. et al. (2005) Helicobacter pylori binds to CD74 on gastric epithelial cells and stimulates interleukin-8 production.
    Infect Immun. 73: 2736-43.
  3. Lapaque, N. et al. (2009) The HLA-DRalpha chain is modified by polyubiquitination.
    J Biol Chem. 284: 7007-16.
  4. van Bergen, J. et al. (1997) Efficient loading of HLA-DR with a T helper epitope by genetic exchange of CLIP.
    Proc Natl Acad Sci U S A. 94: 7499-502.
  5. Barrera, C.A. et al. (2005) Polarized expression of CD74 by gastric epithelial cells.
    J Histochem Cytochem. 53: 1481-9.
  6. Beswick, E.J. et al. (2006) The Helicobacter pylori urease B subunit binds to CD74 on gastric epithelial cells and induces NF-kappaB activation and interleukin-8 production.
    Infect Immun. 74: 1148-55.
  7. Goldenberg, D.M. et al. (2019) Subcutaneous administration of anti-CD74 antibody for systemic lupus erythematosus
    US patent US 20170266281 A1

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