CD74 antibody | Bu45

Product Code Applications Pack Size List Price Your Price Qty
MCA2502
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SDS Safety Datasheet SDS
C * F * FN * IF IP 0.2 mg loader
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loader
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Mouse anti Human CD74 antibody, clone Bu45 recognizes human CD74, a type II transmembrane protein, also known as the MHC class II associated invariant chain. The Bu45 antibody recognizes 33, 35 and 41 kDa isoforms of CD74. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes.

CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. Mouse anti Human CD74 antibody, clone Bu45 binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
B lymphoblastoid cell line HFB1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/25 1/50
Functional Assays 2
Immunofluorescence
Immunohistology - Frozen 3
Immunohistology - Paraffin
Immunoprecipitation
  1. 1 Results maybe enhanced using membrane permeabilisation. Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
  3. 3The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for CD74 antibody

  1. Wraight, C.J. et al. (1990) Human major histocompatibility complex class II invariant chain is expressed on the cell surface.
    J Biol Chem. 265 (10): 5787-92.
  2. Beswick, E.J. et al. (2005) Helicobacter pylori binds to CD74 on gastric epithelial cells and stimulates interleukin-8 production.
    Infect Immun. 73: 2736-43.
  3. Lapaque, N. et al. (2009) The HLA-DRalpha chain is modified by polyubiquitination.
    J Biol Chem. 284: 7007-16.
  4. van Bergen, J. et al. (1997) Efficient loading of HLA-DR with a T helper epitope by genetic exchange of CLIP.
    Proc Natl Acad Sci U S A. 94: 7499-502.
  5. Barrera, C.A. et al. (2005) Polarized expression of CD74 by gastric epithelial cells.
    J Histochem Cytochem. 53: 1481-9.
  6. Beswick, E.J. et al. (2006) The Helicobacter pylori urease B subunit binds to CD74 on gastric epithelial cells and induces NF-kappaB activation and interleukin-8 production.
    Infect Immun. 74: 1148-55.
  7. Goldenberg, D.M. et al. (2019) Subcutaneous administration of anti-CD74 antibody for systemic lupus erythematosus
    US patent US 20170266281 A1

Flow Cytometry

Functional Assays

Immunofluorescence

Immunoprecipitation

RRID
AB_808457
UniProt
P04233
Entrez Gene
CD74
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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