CD68 antibody | Y1/82A
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F WB ResetMouse anti Human CD68
- Product Type
- Monoclonal Antibody
- Clone
- Y1/82A
- Isotype
- IgG2b
- Specificity
- CD68
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Antibody Quality and 
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| Mouse anti Human CD68 antibody, clone Y1/82A recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Phytohaemagglutinin stimulated peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the murine NS1 myeloma.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/10 | 1/100 |
- 1 Results maybe enhanced using membrane permeabilisation. Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2b Negative Control | ||||||
Source Reference
-
Davey, F.R. et al. (1988) Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages.
J Clin Pathol. 41 (7): 753-8.
References for CD68 antibody
-
Micklem, K. et al. (1989) A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.
Br J Haematol. 73 (1): 6-11. -
Pulford, K.A. et al. (1990) Distribution of the CD68 macrophage/myeloid associated antigen.
Int Immunol. 2 (10): 973-80. -
Holness, C.L. & Simmons, D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins.
Blood. 81 (6): 1607-13. -
Mahida, Y.R. et al. (1997) Migration of human intestinal lamina propria lymphocytes, macrophages and eosinophils following the loss of surface epithelial cells.
Clin Exp Immunol. 109 (2): 377-86.
- UniProt
- P34810
- Entrez Gene
- CD68
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005624 membrane fraction
- GO:0005765 lysosomal membrane
- GO:0010008 endosome membrane
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