CD68 antibody | Y1/82A

Mouse anti Human CD68

Product Type
Monoclonal Antibody
Clone
Y1/82A
Isotype
IgG2b
Specificity
CD68

Product Code Applications Pack Size List Price Your Price Qty
MCA6014GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD68 antibody, clone Y1/82A recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Phytohaemagglutinin stimulated peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the murine NS1 myeloma.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/10 1/100
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl

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Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Leucoperm

Source Reference

  1. Davey, F.R. et al. (1988) Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages.
    J Clin Pathol. 41 (7): 753-8.

References for CD68 antibody

  1. Micklem, K. et al. (1989) A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.
    Br J Haematol. 73 (1): 6-11.
  2. Pulford, K.A. et al. (1990) Distribution of the CD68 macrophage/myeloid associated antigen.
    Int Immunol. 2 (10): 973-80.
  3. Holness, C.L. & Simmons, D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins.
    Blood. 81 (6): 1607-13.
  4. Mahida, Y.R. et al. (1997) Migration of human intestinal lamina propria lymphocytes, macrophages and eosinophils following the loss of surface epithelial cells.
    Clin Exp Immunol. 109 (2): 377-86.

UniProt
P34810
Entrez Gene
CD68
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0005765 lysosomal membrane
GO:0010008 endosome membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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