CD68 antibody | Y1/82A

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Anti Human CD68 Antibody, clone Y1/82A thumbnail image 1

Anti Human CD68 Antibody, clone Y1/82A thumbnail image 2

Anti Human CD68 Antibody, clone Y1/82A thumbnail image 3

Anti Human CD68 Antibody, clone Y1/82A gallery image 1 — Figure A. PE conjugated Mouse anti Human CD14 (MCA1568PE) and Alexa Fluor 647 conjugated Mouse IgG2b isotype control (MCA691A647). Figure B. PE conjugated Mouse anti Human CD14 (MCA1568PE) and Alexa Fluor 647 conjugated Mouse anti Human CD68 (**MCA6014A647**). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

Anti Human CD68 Antibody, clone Y1/82A gallery image 2 — Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and FITC conjugated Mouse IgG2b isotype control (MCA691F). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and FITC conjugated Mouse anti Human CD68 (**MCA6014F**). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

Anti Human CD68 Antibody, clone Y1/82A gallery image 3 — Figure A. Pacific Blue conjugated Mouse anti Human CD14 (MCA1568PB) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. Pacific Blue conjugated Mouse anti Human CD14 (MCA1568PB) and RPE conjugated Mouse anti Human CD68 (**MCA6014PE**). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Human CD68:Alexa Fluor® 488

Mouse anti Human CD68:Alexa Fluor® 647

Mouse anti Human CD68:FITC

Mouse anti Human CD68

Mouse anti Human CD68:RPE

Product Type: Monoclonal Antibody
Clone: Y1/82A
Isotype: IgG2b

Product Code	Applications	Pack Size
MCA6014A488	F*	100 Tests/1ml
MCA6014A647	F*	100 Tests/1ml
MCA6014F	F*	0.1 mg
MCA6014GA	F*	0.1 mg
MCA6014PE	F*	100 Tests

Mouse anti Human CD68 antibody, clone Y1/82A recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes.

Product Details

Target Species: Human
Product Form: Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form: Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution: Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free: Yes
Immunogen: Phytohaemagglutinin stimulated peripheral blood mononuclear cells.
Approx. Protein Concentrations: IgG concentration 0.05 mg/ml
Approx. Protein Concentrations: IgG concentration 0.05 mg/ml
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the murine NS1 myeloma.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life: 12 months from date of despatch
Shelf Life: 12 months from date of despatch
Shelf Life: 12 months from date of despatch
Shelf Life: 12 months from date of despatch
Shelf Life: 12 months from date of reconstitution

More Information

UniProt: P34810 Related reagents
Entrez Gene: CD68 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0005624 membrane fraction; GO:0005765 lysosomal membrane; GO:0010008 endosome membrane
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD68 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry ¹		Neat
Flow Cytometry ¹		Neat
Flow Cytometry ¹		Neat
Flow Cytometry ¹	1/10	1/100
Flow Cytometry ¹		Neat

¹ Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

¹ Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

¹ Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

¹ Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

¹ Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10 μl of the suggested working dilution to label 1x10⁶ cells in 100 μl
Flow Cytometry: Use 10 μl of the suggested working dilution to label 1x10⁶ cells in 100 μl
Flow Cytometry: Use 10 μl of the suggested working dilution to label 1x10⁶ cells in 100 μl
Flow Cytometry: Use 10 μl of the suggested working dilution to label 1x10⁶ cells in 100 μl
Flow Cytometry: Use 10 μl of the suggested working dilution to label 1x10⁶ cells in 100 μl. Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG2b:FITC	HCA038F	0.1 mg	F
Human anti Mouse IgG2b:HRP	HCA038P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG2b Negative Control:Alexa Fluor® 488	MCA691A488	100 Tests/1ml	F
Mouse IgG2b Negative Control:Alexa Fluor® 647	MCA691A647	100 Tests/1ml	F
Mouse IgG2b Negative Control:FITC	MCA691F	100 Tests	F
Mouse IgG2b Negative Control	MCA691	100 Tests	F
Mouse IgG2b Negative Control:RPE	MCA691PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Leucoperm™	BUF09	50 Tests	F

Product Specific References

Source Reference

Davey, F.R. et al. (1988) Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages.
J Clin Pathol. 41 (7): 753-8.

References for CD68 antibody

Micklem, K. et al. (1989) A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.
Br J Haematol. 73 (1): 6-11.
Pulford, K.A. et al. (1990) Distribution of the CD68 macrophage/myeloid associated antigen.
Int Immunol. 2 (10): 973-80.
Holness, C.L. & Simmons, D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins.
Blood. 81 (6): 1607-13.
Mahida, Y.R. et al. (1997) Migration of human intestinal lamina propria lymphocytes, macrophages and eosinophils following the loss of surface epithelial cells.
Clin Exp Immunol. 109 (2): 377-86.

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