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CD68 antibody | Y1/82A

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Anti Human CD68 Antibody, clone Y1/82A thumbnail image 1 Anti Human CD68 Antibody, clone Y1/82A thumbnail image 2 Anti Human CD68 Antibody, clone Y1/82A thumbnail image 3
Anti Human CD68 Antibody, clone Y1/82A gallery image 1
Figure A. PE conjugated Mouse anti Human CD14 (MCA1568PE) and Alexa Fluor 647 conjugated Mouse IgG2b isotype control (MCA691A647). Figure B. PE conjugated Mouse anti Human CD14 (MCA1568PE) and Alexa Fluor 647 conjugated Mouse anti Human CD68 (MCA6014A647). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Human CD68 Antibody, clone Y1/82A gallery image 2
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and FITC conjugated Mouse IgG2b isotype control (MCA691F). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and FITC conjugated Mouse anti Human CD68 (MCA6014F). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Human CD68 Antibody, clone Y1/82A gallery image 3
Figure A. Pacific Blue conjugated Mouse anti Human CD14 (MCA1568PB) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. Pacific Blue conjugated Mouse anti Human CD14 (MCA1568PB) and RPE conjugated Mouse anti Human CD68 (MCA6014PE). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Human CD68:Alexa Fluor® 488

Mouse anti Human CD68:Alexa Fluor® 647

Mouse anti Human CD68:FITC

Mouse anti Human CD68

Mouse anti Human CD68:RPE

Product Type
Monoclonal Antibody
Clone
Y1/82A
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA6014A488
Datasheet
F*
MSDS
100 Tests/1ml loader

MCA6014A647
Datasheet
F*
MSDS
100 Tests/1ml loader

MCA6014F
Datasheet
F*
MSDS
0.1 mg loader

MCA6014GA
Datasheet
F*
MSDS
0.1 mg loader

MCA6014PE
Datasheet
F*
MSDS
100 Tests loader

Mouse anti Human CD68 antibody, clone Y1/82A recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Phytohaemagglutinin stimulated peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the murine NS1 myeloma.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P34810
Entrez Gene
CD68
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0005765 lysosomal membrane
GO:0010008 endosome membrane
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD68 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
Flow Cytometry 1 Neat
Flow Cytometry 1 Neat
Flow Cytometry 1 1/10 1/100
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl. Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2b Negative Control:Alexa Fluor® 488 MCA691A488 F 100 Tests/1ml loader
Mouse IgG2b Negative Control:Alexa Fluor® 647 MCA691A647 F 100 Tests/1ml loader
Mouse IgG2b Negative Control:FITC MCA691F F 100 Tests loader
Mouse IgG2b Negative Control MCA691 F 100 Tests loader
Mouse IgG2b Negative Control:RPE MCA691PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Leucoperm™ BUF09 F 50 Tests

Product Specific References

Source Reference

  1. Davey, F.R. et al. (1988) Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages.
    J Clin Pathol. 41 (7): 753-8.

References for CD68 antibody

  1. Micklem, K. et al. (1989) A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.
    Br J Haematol. 73 (1): 6-11.
  2. Pulford, K.A. et al. (1990) Distribution of the CD68 macrophage/myeloid associated antigen.
    Int Immunol. 2 (10): 973-80.
  3. Holness, C.L. & Simmons, D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins.
    Blood. 81 (6): 1607-13.
  4. Mahida, Y.R. et al. (1997) Migration of human intestinal lamina propria lymphocytes, macrophages and eosinophils following the loss of surface epithelial cells.
    Clin Exp Immunol. 109 (2): 377-86.
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