CD62L antibody | FMC46
Fitc conjugated Mouse anti Human CD62L antibody, clone FMC46 (MCA1076F) used to evaluate CD62L expression levels on human neutrophils and monocytes by flow cytometry.
Image caption:
Effect of flexible ureterenoscopy on CD62L cell surface expression of neutrophils and monocytes. The points represent mean ± SD.
From: Hughes SF, Moyes AJ, Lamb RM, Ella-Tongwiis P, Snyper NYF, Shergill I.
The role of phagocytic leukocytes following flexible ureterenoscopy, for the treatment of kidney stones: an observational, clinical pilots-study.
Eur J Med Res. 2020 Dec 11;25(1):68.
doi: 10.1186/s40001-020-00466-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Human CD62L antibody, clone FMC46 (MCA1076F) used to assess CD62L expression on bovine peripheral blood mononuclear cells by flow cytometry.
Image caption:
Induction of central memory Tp1+/CD8+ cells.
b A live example of pseudo color plots of CD44 and CD62L expression from total lymphocytes (left) and from Tp1+/CD8+-gated cells from one animal in group 1, 7 days post-MVA boost (right).
From:Svitek N, Saya R, Awino E, Munyao S, Muriuki R, Njoroge T, Pellé R, Ndiwa N, Poole J, Gilbert S, Nene V, Steinaa L.
An Ad/MVA vectored Theileria parva antigen induces schizont-specific CD8+ central memory T cells and confers partial protection against a lethal challenge.
NPJ Vaccines. 2018 Sep 11;3:35.
doi: 10.1038/s41541-018-0073-5..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F P ResetMouse anti Human CD62L
- Product Type
- Monoclonal Antibody
- Clone
- FMC46
- Isotype
- IgG2b
- Specificity
- CD62L
Antibody Quality and Performance Guarantee
| Mouse anti Human CD62L antibody, clone FMC46 recognizes human CD62L, also known a L-selectin, a 74-95 kDa member of the selectin family of adhesion receptors, which acts as a ligand for both CD62P (P-selectin) and CD62E (E-selectin). Human CD62L is constitutively expressed on most leucocytes including monocytes, granulocytes, lymphocytes, NK cells, bone marrow myeloid progenitor cells and on a subset of thymocytes. CD62L plays an important role in leucocyte tethering and rolling on the endothelial cell surface and for the homing of naïve lymphocytes to lymph nodes and Peyers patches via HEV. Neutrophils require a constant supply of this molecule on the cell surface for migration into peripheral tissues and adhesion to activated endothelium at sites of inflammation, where CD62L is rapidly shed as soluble L-selectin, but surface expression still remains. The expression of CD62L is down regulated on lymphocytes and neutrophils by PMA stimulation. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Cynomolgus monkey Rhesus Monkey Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- MCA1076G: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA1076GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- PHA stimulated lymphoblasts
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
10 ug/ml | |
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- Histology Positive Control Tissue
- Human tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG2b Negative Control | ||||||
References for CD62L antibody
-
Pilarski, L.M. et al. (1991) FMC46, a cell protrusion-associated leukocyte adhesion molecule-1 epitope on human lymphocytes and thymocytes.
J Immunol. 147 (1): 136-43. -
Zola, H. et al. (1991) The expression of sub-population markers on B cells: a re-evaluation using high-sensitivity fluorescence flow cytometry.
Dis Markers. 9 (2): 103-18. -
Sopp, P. & Howard, C.J. (1997) Cross-reactivity of monoclonal antibodies to defined human leucocyte differentiation antigens with bovine cells.
Vet Immunol Immunopathol. 56 (1-2): 11-25. -
Haanstra, K.G. et al. (2008) Characterization of naturally occurring CD4+CD25+ regulatory T cells in rhesus monkeys.
Transplantation 85:1185-92. -
Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
Blood. 112 (6): 2512-9. -
Raposo, R.A. et al. (2011) Protein Kinase C and NF-{kappa}B-Dependent CD4 Downregulation in Macrophages Induced by T Cell-Derived Soluble Factors: Consequences for HIV-1 Infection.
J Immunol. 187: 748-59. -
Hughes, S.F. et al. (2010) Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers.
J Inflamm (Lond). 7:2. -
Bismarck, D. et al. (2012) Canine CD4+CD8+ double positive T cells in peripheral blood have features of activated T cells.
Vet Immunol Immunopathol. 149: 157-66.
View The Latest Product References
-
Hartley, A.N. & Tarleton, R.L. (2015) Chemokine receptor 7 (CCR7)-expression and IFNγ production define vaccine-specific canine T-cell subsets.
Vet Immunol Immunopathol. 164 (3-4): 127-36. -
Hayhoe, R.P. et al. (2006) Annexin 1 and its bioactive peptide inhibit neutrophil-endothelium interactions under flow: indication of distinct receptor involvement.
Blood. 107 (5): 2123-30. -
Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther. 321 (2): 656-62. -
Aspinall, A.I. et al. (2010) CX(3)CR1 and vascular adhesion protein-1-dependent recruitment of CD16(+) monocytes across human liver sinusoidal endothelium.
Hepatology. 51 (6): 2030-9. -
Rothe, K. et al. (2017) Canine peripheral blood CD4+CD8+ double-positive Tcell subpopulations exhibit distinct Tcell phenotypes and effector functions.
Vet Immunol Immunopathol. 185: 48-56. -
Withers, S.S. et al. (2018) Multi-color flow cytometry for evaluating age-related changes in memory lymphocyte subsets in dogs.
Dev Comp Immunol. 87: 64-74. -
Hughes, S.F. et al. (2020) The role of phagocytic leukocytes following flexible ureterenoscopy, for the treatment of kidney stones: an observational, clinical pilots-study.
Eur J Med Res. 25 (1): 68. -
Svitek, N. et al. (2018) An Ad/MVA vectored Theileria parva antigen induces schizont-specific CD8+ central memory T cells and confers partial protection against a lethal challenge.
NPJ Vaccines. 3: 35. -
Tucker, N. et al. (2023) Bovine blood and milk T-cell subsets in distinct states of activation and differentiation during subclinical Staphylococcus aureus mastitis.
J Reprod Immunol. 156: 103826.
- Synonyms
- LECAM-1
- L-SELECTIN
- RRID
- AB_321521
- UniProt
- P14151
- Entrez Gene
- SELL
- GO Terms
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
- GO:0008201 heparin binding
- GO:0043208 glycosphingolipid binding
- GO:0050776 regulation of immune response
- GO:0050900 leukocyte migration
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