CD45RB antibody | MEM-55
Mouse anti Human CD45RB
- Product Type
- Monoclonal Antibody
- Clone
- MEM-55
- Isotype
- IgG1
- Specificity
- CD45RB
Antibody Quality and Performance Guarantee
| Mouse anti Human CD45RB antibody, clone MEM-55 recognizes the isoform of the leucocyte common antigen encoded by exon B and stains B cells, most T cells and monocytes. Mouse anti Human CD45RB antibody, clone MEM-55 immunoprecipitates bands of 190, 200 and 205 kDa. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey Horse Goat Llama Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
1/50 | 1/200 |
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD45RB antibody
-
Blaylock, M.G. et al. (2003) Eosinophils from patients with asthma express higher levels of the pan-leucocyte receptor CD45 and the isoform CD45RO.
Clin Exp Allergy. 33: 936-41. -
Windhagen, A. et al. (2007) Altered CD45 isoform expression in C77G carriers influences cytokine responsiveness and adhesion properties of T cells.
Clin Exp Immunol. 150: 509-17. -
Ibrahim, S. et al. (2007) Screening of anti-human leukocyte monoclonal antibodies for reactivity with equine leukocytes.
Vet Immunol Immunopathol. 119: 63-80. -
Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
Vet Immunol Immunopathol. 119: 123-30. -
Ponchel, F. et al. (2014) An immunological biomarker to predict MTX response in early RA.
Ann Rheum Dis. 73 (11): 2047-53. -
Khan, A.A. et al. (2015) Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local PD-1+ TIM-3+ CD8+ exhausted T cells.
J Virol. 89 (13): 6619-32. -
Hunt, L. et al. (2016) T cell subsets: an immunological biomarker to predict progression to clinical arthritis in ACPA-positive individuals.
Ann Rheum Dis. 75 (10): 1884-9.
- RRID
- AB_321430
- UniProt
- P08575
- Entrez Gene
- PTPRC
- GO Terms
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0002378 immunoglobulin biosynthetic process
- GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0007411 axon guidance
- View More GO Terms
- GO:0019901 protein kinase binding
- GO:0030890 positive regulation of B cell proliferation
- GO:0033261 regulation of S phase
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
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