CD8 antibody | YTC182.20
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used to identify CD8+ expressing cells in the endocervix of Rhesus macaques (Macaca mulatta) by immunofluorescence.
Image caption:
Most CD3+ T cells were CD8+ in genital lymphoid aggregates.
Representative images of endocervix section stained with Mamu-A1/Gag tetramers (red), CD8 antibodies (green), and CD3 antibodies (blue) from animal #338.02. Tetramer+ cells are indicated with white arrows in the enlargement. The bottom panels show individual tetramer and antibody stains. Note most CD3+ T cells are CD8+. The top left panel shows a montage of projected z-series of several 200× fields. Scale bars = 100μm.
From: Sasikala-Appukuttan AK, Kim HO, Kinzel NJ, Hong JJ,
Smith AJ, Wagstaff R, et al. (2013)
Location and Dynamics of the Immunodominant CD8 T Cell Response to SIVΔnef Immunization and SIVmac251 Vaginal Challenge.
PLoS ONE 8(12): e81623.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used to identify CD8+ expressing cells in the lymphoid organs of Rhesus macaques (Macaca mulatta) by immunofluorescence.
Image caption:
Localization of SIV-specific CD8+ T cells in lymph nodes and spleen.
The representative images shown in B) are from a spleen section from an animal (119.96) at 5 weeks post-SIVΔnef vaccination. Mamu-A1/Gag tetramer+ cells are red to pink colored, CD8+ T cells are blue, and CD20+ B cells are green. Tetramer+CD8+ cells localized with other CD8 T cells and were most abundant in the red pulp (RP) and marginal zones (MZ), with relatively fewer located in T cell zones (T) and in B cell follicles (B). The left panel shows a montage of several confocal microscope fields of projected Z-scans stitched together, collected with a 10× objective. Enlargements show individual confocal z-scans of single 200× fields.
From: Sasikala-Appukuttan AK, Kim HO, Kinzel NJ, Hong JJ,
Smith AJ, Wagstaff R, et al. (2013)
Location and Dynamics of the Immunodominant CD8 T Cell Response to SIVΔnef Immunization and SIVmac251 Vaginal Challenge.
PLoS ONE 8(12): e81623.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used for evaluation of CD8 localization in multiple sclerotic brain sections by immunofluorescence.
Image caption:
Representative maximum intensity projections of z-stack laser confocal microscopy images of immunofluorescent triple stainings for grB (green color), CD8 (red color) and the early apoptotic cell marker “cleaved caspase-3” (cCASP3; white color). Stained sections were counterstained with DAPI (blue color). Representative stainings of three mAIL are shown of 12 immunohistochemically classified WML tissues of 10 MS patients analyzed. Punctated (inset 1) and polarized grB expression by CD8+ T cells (insets 3 and 5), as well as grB-negative CD8+ T cells are shown (insets 2, 4 and 6). Co-localization of grB and cCASP3 is observed in a cell adjacent to a CD8+ T-cell with polarized grB suggesting grB-mediated killing of the respective target cell (inset 5). Dotted line represents the glia limitans separating the perivascular space and parenchyma.
From: van Nierop GP, van Luijn MM, Michels SS, Melief MJ, Janssen M, Langerak AW, Ouwendijk WJD, Hintzen RQ, Verjans GMGM.
Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
Acta Neuropathol. 134(3):383-401.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used to identify CD8+ expressing cells in the vagina of Rhesus macaques (Macaca mulatta) by immunofluorescence.
Image caption:
Localization of SIV-specific CD8 T cells in vagina at 14 days post-vaccination.
Representative confocal image showing Mamu-A1/Gag tetramer+ cells (red), CD8 (blue), and CD20 (green). The right panel shows a montage of projected z-series of several 200× fields with the epithelium (E), lamina propria (LP), muscularis (M), and areas with vessels (V) indicated. In the enlarged image on the left, tetramer+ cells are indicated with arrows. Scale bars are 100μm.
From: Sasikala-Appukuttan AK, Kim HO, Kinzel NJ, Hong JJ,
Smith AJ, Wagstaff R, et al. (2013)
Location and Dynamics of the Immunodominant CD8 T Cell Response to SIVΔnef Immunization and SIVmac251 Vaginal Challenge.
PLoS ONE 8(12): e81623.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used for evaluation of CD8 localization in multiple sclerotic brain sections by immunofluorescence.
Image caption:
CD8+ T cells interact with all major brain-resident cell types in white matter lesions of MS patients. Representative double-immunofluorescence stainings on 8-μm sections of 12 formalin-fixed paraffin-embedded white matter lesion (WML) tissues from 10 MS patients are shown for: ionized calcium-binding adapter molecule 1 (Iba1: marker for microglia - shown green).
From: van Nierop GP, van Luijn MM, Michels SS, Melief MJ, Janssen M, Langerak AW, Ouwendijk WJD, Hintzen RQ, Verjans GMGM.
Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
Acta Neuropathol. 134(3):383-401.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used for evaluation of CD8 localization in multiple sclerotic brain sections by immunofluorescence.
Image caption:
CD8+ T cells interact with all major brain-resident cell types in white matter lesions of MS patients. Representative double-immunofluorescence stainings on 8-μm sections of 12 formalin-fixed paraffin-embedded white matter lesion (WML) tissues from 10 MS patients are shown for: proteolipid protein (MCA839G) (PLP: marker for oligodendrocytes - shown green)
From: van Nierop GP, van Luijn MM, Michels SS, Melief MJ, Janssen M, Langerak AW, Ouwendijk WJD, Hintzen RQ, Verjans GMGM.
Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
Acta Neuropathol. 134(3):383-401.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used for evaluation of CD8 localization in multiple sclerotic brain sections by immunofluorescence.
Image caption:
CD8+ T cells interact with all major brain-resident cell types in white matter lesions of MS patients. Representative double-immunofluorescence stainings on 8-μm sections of 12 formalin-fixed paraffin-embedded white matter lesion (WML) tissues from 10 MS patients are shown for: neurofilament heavy chain (NF-H: marker for neurons - shown green)
From: van Nierop GP, van Luijn MM, Michels SS, Melief MJ, Janssen M, Langerak AW, Ouwendijk WJD, Hintzen RQ, Verjans GMGM.
Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
Acta Neuropathol. 134(3):383-401.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used for evaluation of CD8 localization in multiple sclerotic brain sections by immunofluorescence.
Image caption:
CD8+ T cells interact with all major brain-resident cell types in white matter lesions of MS patients. Representative double-immunofluorescence stainings on 8-μm sections of 12 formalin-fixed paraffin-embedded white matter lesion (WML) tissues from 10 MS patients are shown for: glial fibrillary acidic protein (GFAP: marker for astrocytes - shown green).
From: van Nierop GP, van Luijn MM, Michels SS, Melief MJ, Janssen M, Langerak AW, Ouwendijk WJD, Hintzen RQ, Verjans GMGM.
Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
134(3):383-401.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used to identify CD8+ expressing cells in the lymphoid organs of Rhesus macaques (Macaca mulatta) by immunofluorescence.
Image caption:
Localization of SIV-specific CD8+ T cells in lymph nodes and spleen.
The representative images shown in are A) from an axillary lymph node from an animal (119.96) 5 weeks post-SIV-Δnef vaccination. Mamu-A1/Tat tetramer+ cells are red to pink, CD8+ T cells are green, and CD3+ T cells are blue. The left panel shows a montage of several fields containing projected z-scans collected with a confocal microscope 20× objective. Enlargements to the right show individual fields of projected z-scans collected with a 60× objective.
red pulp (RP) marginal zones (MZ) T cell zones (T) B cell follicles (B).
From: Sasikala-Appukuttan AK, Kim HO, Kinzel NJ, Hong JJ,
Smith AJ, Wagstaff R, et al. (2013)
Location and Dynamics of the Immunodominant CD8 T Cell Response to SIVΔnef Immunization and SIVmac251 Vaginal Challenge.
PLoS ONE 8(12): e81623.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used to identify CD8+ expressing cells in the Peyer's patches of Rhesus macaques (Macaca mulatta) by immunofluorescence.
Image caption:
Localization of SIV-specific CD8 T cells in ileal Peyer's patches.
The representative images shown are from animal #174.96 stained with Mamu-A1/gag tetramers (red), CD20 antibodies (green), and CD3 antibodies (blue). Tetramer+ cells were localized throughout the Peyer's patches of ileum tissue sections with other T cells around as well as within B cell follicles. The left shows a montage of several confocal Z-scan fields stitched together, collected with a 20× objective.
From: Sasikala-Appukuttan AK, Kim HO, Kinzel NJ, Hong JJ,
Smith AJ, Wagstaff R, et al. (2013)
Location and Dynamics of the Immunodominant CD8 T Cell Response to SIVΔnef Immunization and SIVmac251 Vaginal Challenge.
PLoS ONE 8(12): e81623.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used to identify CD8+ expressing cells in the endocervix of Rhesus macaques (Macaca mulatta) by immunofluorescence.
Image caption:
Localization of SIV-specific CD8+ T cells in cervix and vagina.
A) Top panels show representative images of endocervix from an animal (137.09) at 5 weeks post-SIV-Δnef vaccination as examples of the low end of the spectrum of levels of tetramer+ detected in genital tissues amongst the whole cohort. Mamu-A1/Gag tetramer+ cells are red to pink, CD8+ T cells are blue, and CD20+ cells are green. B) In the bottom panels Mamu-A1/Tat MHC-tetramer-binding cells are red to pink, CD3+ T cells are blue, and CD20+ B cells are green from a representative image of vagina from animal #338.02, 21 weeks post-SIV-Δnef vaccination and 7 days following vaginal challenge with WT-SIV, as an example of the higher end of the spectrum of levels of tetramer+ cells detected in genital tissues amongst the whole cohort. The edge of the epithelium (E) is indicated with a white line; lamina propria indicated by LP. For both A) and B), the left panel shows a montage of several confocal projected z-series images (projected z-series are images collected a different depths stacked on top of each other), collected with a 20× objective. The right panels shows enlargements with several red to pink tetramer+ cells evident (indicated by arrows in A) localized diffusely as well as within small T cell aggregates. Scale bars = 100μm.
From: Sasikala-Appukuttan AK, Kim HO, Kinzel NJ, Hong JJ,
Smith AJ, Wagstaff R, et al. (2013)
Location and Dynamics of the Immunodominant CD8 T Cell Response to SIVΔnef Immunization and SIVmac251 Vaginal Challenge.
PLoS ONE 8(12): e81623.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD8:FITC
- Product Type
- Monoclonal Antibody
- Clone
- YTC182.20
- Isotype
- IgG2b
- Specificity
- CD8
Antibody Quality and Performance Guarantee
| Rat anti Human CD8 antibody, clone YTC182.20 recognizes the human CD8 cell surface antigen, a ~30/32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Mouse L cells transfected with human CD8 gene.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag.1.2.3 myeloma line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
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References for CD8 antibody
-
Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocytes.
Leucocyte Typing IV. Oxford University Press p 1058-1063. -
Walton, L.J. et al. (1998) Intra-epithelial subpopulations of T lymphocytes and Langerhans cells in oral lichen planus.
J Oral Pathol Med. 27 (3): 116-23. -
Shacklett, B.L. et al. (2004) Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT: implications for immune control of HIV-1 infection.
J Immunol. 173 (1): 641-8. -
Salajegheh, M. et al. (2007) Upregulation of thrombospondin-1(TSP-1) and its binding partners, CD36 and CD47, in sporadic inclusion body myositis.
J Neuroimmunol. 187 (1-2): 166-74. -
Salajegheh, M. et al. (2007) T cell receptor profiling in muscle and blood lymphocytes in sporadic inclusion body myositis.
Neurology. 69 (17): 1672-9. -
Sasikala-Appukuttan, A.K. et al. (2013) Location and dynamics of the immunodominant CD8 T cell response to SIVΔnef immunization and SIVmac251 vaginal challenge.
PLoS One. 8 (12): e81623. -
Willing, A. et al. (2014) CD8⁺ MAIT cells infiltrate into the CNS and alterations in their blood frequencies correlate with IL-18 serum levels in multiple sclerosis.
Eur J Immunol. 44 (10): 3119-28. -
Wang, H. et al. (2016) Assessment of placental transfer and the effect on embryo-fetal development of a humanized monoclonal antibody targeting lymphotoxin-alpha in non-human primates.
Reprod Toxicol. 63: 82-95.
View The Latest Product References
-
Cramer, M.L. et al. (2017) Induction of T-Cell Infiltration and Programmed Death Ligand 2 Expression by Adeno-Associated Virus in Rhesus Macaque Skeletal Muscle and Modulation by Prednisone.
Hum Gene Ther. 28 (6): 493-509. -
van, G.N. et al. (2017) Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
Acta Neuropathol. 134 (3): 383-401.
- RRID
- AB_321404
- UniProt
- P01732
- P10966
- Entrez Gene
- CD8A
- CD8B
- GO Terms
- GO:0005576 extracellular region
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005887 integral to plasma membrane
- GO:0015026 coreceptor activity
- GO:0019882 antigen processing and presentation
- GO:0042101 T cell receptor complex
- GO:0042288 MHC class I protein binding
- GO:0050776 regulation of immune response
MCA351F
151791
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