CD32 antibody | AT10

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MCA1075
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C * F FN * IP 0.2 mg loader
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MCA1075T
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C * F IP 25 µg loader
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Mouse anti Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.

Mouse anti Human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII (Larsson et al. 1997).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Rhesus Monkey
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.09% sodium azide (NaN3).
Immunogen
K562 cell line.
Approx. Protein Concentrations
IgG concentration 1 mg/ml.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 20ug/ml
Functional Assays 1
Immunohistology - Frozen 2 1/500 1/1000
Immunohistology - Paraffin
Immunoprecipitation 20ug/ml
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
  2. 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or cells or 100μl whole blood.
Histology Positive Control Tissue
Lymph node.

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Source Reference

  1. Greenman, J. et al. (1991) Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.
    Mol Immunol. 28 (11): 1243-54.

References for CD32 antibody

  1. Pawar, P. et al. (2004) Fluid shear regulates the kinetics and receptor specificity of Staphylococcus aureus binding to activated platelets.
    J Immunol. 173: 1258-65.
  2. Su, K. et al. (2004) A promoter haplotype of the immunoreceptor tyrosine-based inhibitory motif-bearing FcgammaRIIb alters receptor expression and associates with autoimmunity. II. Differential binding of GATA4 and Yin-Yang1 transcription factors and correlated receptor expression and function.
    J Immunol. 172: 7192-9.
  3. van der Heijden, J. et al. (2012) Phenotypic variation in IgG receptors by nonclassical FCGR2C alleles.
    J Immunol. 188 (3): 1318-24.
  4. Pellerin, A. et al. (2015) Anti-BDCA2 monoclonal antibody inhibits plasmacytoid dendritic cell activation through Fc-dependent and Fc-independent mechanisms.
    EMBO Mol Med. 7 (4): 464-76.
  5. Van Den Herik-Oudijk, I.E. et al. (1994) Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes.
    J Immunol. 152 (2): 574-85.
  6. Lilliehöök, I. et al. (1998) Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies.
    Vet Immunol Immunopathol. 61 (2-4): 181-93.
  7. Larsson M et al. (1997) Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro.
    Immunology. 90 (1): 138-46.
  8. Mold, C. and Du Clos, T.W. (2006) C-reactive protein increases cytokine responses to Streptococcus pneumoniae through interactions with Fc gamma receptors.
    J Immunol. 176: 7598-604.
  9. View The Latest Product References
  10. Dutertre, C.A. et al. (2008) A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function.
    J Leukoc Biol. 84 (6): 1511-20.
  11. Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells.
    Vet Immunol Immunopathol. 135: 188-98.
  12. Sims, G.P. et al. (2005) Identification and characterization of circulating human transitional B cells.
    Blood. 105: 4390-8.
  13. Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.
    J Exp Med. 203: 1629-35.
  14. Zhao, X.W. et al. (2011) CD47-signal regulatory protein-α (SIRPα) interactions form a barrier for antibody-mediated tumor cell destruction.
    Proc Natl Acad Sci U S A. 108 (45): 18342-7.
  15. Araújo, M.S.et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
    Vet Immunol Immunopathol. 141: 64-75.
  16. Bonnefont-Rebeix, C. et al. (2006) CD86 molecule is a specific marker for canine monocyte-derived dendritic cells.
    Vet Immunol Immunopathol. 109 (1-2): 167-76.
  17. Santer, D.M. et al. (2010) C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes.
    J Immunol. 185: 4738-49.
  18. Shannon, O. et al. (2010) Platelet activation and biofilm formation by Aerococcus urinae, an endocarditis-causing pathogen.
    Infect Immun. 78: 4268-75.
  19. Ito, T. et al. (1999) A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells.
    J Immunol. 163: 1409-19.
  20. Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
    Vet Parasitol. 220: 33-45.
  21. Gazendam, R.P. et al. (2016) Impaired killing of Candida albicans by granulocytes mobilized for transfusion purposes: a role for granule components.
    Haematologica. 101 (5): 587-96.
  22. Liu M et al. (2011) Vitellogenin mediates phagocytosis through interaction with FcγR.
    Mol Immunol. 49 (1-2): 211-8.
  23. Petersson, F. et al. (2018) Platelet activation and aggregation by the opportunistic pathogen Cutibacterium (Propionibacterium) acnes.
    PLoS One. 13 (1): e0192051.
  24. Kahn, F. et al. (2008) Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response.
    PLoS Pathog. 4 (9): e1000149.
  25. Bruggeman, C.W. et al. (2019) Tissue-specific expression of IgG receptors by human macrophages ex vivo.
    PLoS One. 14 (10): e0223264.
  26. Chen, T. et al. (2020) Capsular glycan recognition provides antibody-mediated immunity against tuberculosis.
    J Clin Invest. 130 (4): 1808-22.
  27. Teuben, M.P.J. et al. (2021) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.
    Eur J Trauma Emerg Surg. Jun 10 [Epub ahead of print].

Blocking assay

Flow Cytometry

Functional Assays

Synonyms
FcRII
RRID
AB_321659
UniProt
P12318
P31994
P31995
Entrez Gene
FCGR2A
FCGR2B
FCGR2C
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0019864 IgG binding
GO:0006955 immune response
GO:0044419 interspecies interaction between organisms
GO:0050776 regulation of immune response
GO:0004888 transmembrane receptor activity
GO:0005737 cytoplasm
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