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CD32 antibody | AT10

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Anti Human CD32 Antibody, clone AT10 thumbnail image 1 Anti Human CD32 Antibody, clone AT10 thumbnail image 2 Anti Human CD32 Antibody, clone AT10 thumbnail image 3 Anti Human CD32 Antibody, clone AT10 thumbnail image 4 Anti Human CD32 Antibody, clone AT10 thumbnail image 5 Anti Human CD32 Antibody, clone AT10 thumbnail image 6 Anti Human CD32 Antibody, clone AT10 thumbnail image 7 Anti Human CD32 Antibody, clone AT10 thumbnail image 8 Anti Human CD32 Antibody, clone AT10 thumbnail image 9 Anti Human CD32 Antibody, clone AT10 thumbnail image 10 Anti Human CD32 Antibody, clone AT10 thumbnail image 11 Anti Human CD32 Antibody, clone AT10 thumbnail image 12 Anti Human CD32 Antibody, clone AT10 thumbnail image 13
Anti Human CD32 Antibody, clone AT10 gallery image 1
Staining of human peripheral blood granulocytes with Mouse anti Human CD32 (MCA1075).
Anti Human CD32 Antibody, clone AT10 gallery image 2
Staining of human peripheral blood granulocytes with Mouse anti Human CD32:Alexa Fluor® 647 (MCA1075A647).
Anti Human CD32 Antibody, clone AT10 gallery image 3
Staining of human peripheral blood granulocytes with Mouse anti Human CD32:FITC (MCA1075F).
Anti Human CD32 Antibody, clone AT10 gallery image 4
Staining of human peripheral blood granulocytes with Mouse anti Human CD32:RPE (MCA1075PE).
Anti Human CD32 Antibody, clone AT10 gallery image 5
Staining of human peripheral blood monocytes with Mouse anti Human CD32: Biotin (MCA1075B)
Anti Human CD32 Antibody, clone AT10 gallery image 6
Published customer image:
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used for blocking of Fc receptor and inhibition of IC-mediated α ifn production by pDSs
Image caption:
The Fc region of 24F4A enhances the inhibition of immune complex‐induced IFNα production by human pDCs A–F Isolated human pDCs were treated with increasing concentrations of 24F4A (red circles), 24F4A‐ef (black squares), or 10 μg/ml of the isotype control (black triangle) and stimulated with 5 μM of R848 (A and B), 1 μM of CpG‐A (C and D), or immune complexes (E and F) for 16 h at 37°C. IFNα concentrations in cultured supernatants were determined using ELISA. Representative plots for each stimulation (A, C and E) and the average IC50 of 24F4A and 24F4A‐ef for each stimulation condition (B, D and F) are shown (n = 3). Horizontal bars represent mean IC50, and error bars represent SD of the mean from three independent experiments. G. Isolated human pDCs were treated with 10 μg/ml of 24F4A (black line), 24F4A‐ef (gray shaded histogram), or the isotype control (gray dotted line) for 10 min at 37°C. Flow cytometry was used to evaluate pSYK and pPLCγ2. Shown is a representative plot of two independent experiments. H. Isolated pDCs treated with 10 μg/ml of anti‐CD32a (AT10) and stimulated with CpG‐A, R848, or immune complexes. Error bars represent SD of percent inhibition of IFNα from three independent experiments.

From: Pellerin A, Otero K, Czerkowicz JM, Kerns HM, Shapiro RI, Ranger AM, Otipoby KL, Taylor FR, Cameron TO, Viney JL, Rabah D.
Anti-BDCA2 monoclonal antibody inhibits plasmacytoid dendritic cell activation through Fc-dependent and Fc-independent mechanisms.
EMBO Mol Med. 2015 Mar 11. pii: e201404719.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD32 Antibody, clone AT10 gallery image 7
Detection of CD32 in normal human peripheral blood cells using the Bio-Rad S3 Cell Sorter. 1x106 cells were suspended in 100μL buffer and stained with 10μL Mouse anti Human CD32:RPE (MCA1075PE). Lymphocytes were gated for analysis (A): Histogram overlay with stained cells represented in green and unstained control cells in grey. (B): Density plot of CD32 cells.
Anti Human CD32 Antibody, clone AT10 gallery image 8
Published customer image:
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used as a blocking agent to assess the effect of FcγRs on heparin binding protein (HBP) release by ELISA.
Image caption:
Antibodies against FcγRII inhibit HBP release.
Whole blood (diluted 1∶10) was incubated with antibodies against different FcγRs, isotype control antibodies, or buffer alone, for 20 minutes at 37°C. M1 protein was added and the HBP release was measured. Data are expressed as percentage of release without any antibodies added, and are the mean±SD from three different individuals, each done in duplicate. The different treatment groups were compared using ANOVA, and when found significantly different the treatment groups were compared against the control group using Dunnett's 2 sided test.

From: Kahn F, Mörgelin M, Shannon O, Norrby-Teglund A, Herwald H, Olin AI, et al. (2008)
Antibodies against a Surface Protein of Streptococcus pyogenes Promote a Pathological Inflammatory Response. PLoS Pathog 4(9): e1000149.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD32 Antibody, clone AT10 gallery image 9
Published customer image:
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used to evaluate FcγRII (CD32) expression on Kupffer cells by immunofluorescence on acetone fixed cryosections.
Image caption:
Immunofluorescence stainings of liver sections with CD163 and FcγRII. Magnification 20x. Scale bars equal 50 μm. Figures are representative of n = 2 livers from different donors.

From: Bruggeman CW, Houtzager J, Dierdorp B, Kers J, Pals ST, Lutter R, et al. (2019)
Tissue-specific expression of IgG receptors by human macrophages ex vivo.
PLoS ONE 14(10): e0223264.
doi: 10.1371/journal.pone.0223264
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD32 Antibody, clone AT10 gallery image 10
Published customer image:
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used to evaluate FcγRII (CD32) expression on alveolar macrophages by immunofluorescence on acetone fixed lung cryosections.
Image caption:
Lung macrophages express FcγRI and FcγRII.
Immunofluorescence stainings of lung sections with CD163, Hoechst and FcγRII. Magnification 20x. Scale bars equal 50 μm. Figures are representative of n = 3 lungs from different donors.

From: Bruggeman CW, Houtzager J, Dierdorp B, Kers J, Pals ST, Lutter R, et al. (2019)
Tissue-specific expression of IgG receptors by human macrophages ex vivo.
PLoS ONE 14(10): e0223264.
doi: 10.1371/journal.pone.0223264
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD32 Antibody, clone AT10 gallery image 11
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and Amethyst Orange conjugated Mouse IgG1 isotype control (MCA928AMO). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and Amethyst Orange conjugated Mouse anti Human CD32 (MCA1075AMO). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD32 Antibody, clone AT10 gallery image 12
Published customer image:
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used to evaluate FcγRII (CD32) expression on alveolar macrophages by immunofluorescence on acetone fixed lung cryosections.
Image caption:
Lung macrophages express FcγRI and FcγRII.
Immunofluorescence stainings of lung sections with CD163, Hoechst and FcγRII. Magnification 20x. Scale bars equal 50 μm. Figures are representative of n = 3 lungs from different donors.

From: Bruggeman CW, Houtzager J, Dierdorp B, Kers J, Pals ST, Lutter R, et al. (2019)
Tissue-specific expression of IgG receptors by human macrophages ex vivo.
PLoS ONE 14(10): e0223264.
doi: 10.1371/journal.pone.0223264
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD32 Antibody, clone AT10 gallery image 13
Published customer image:
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used to evaluate FcγRII (CD32) expression on alveolar macrophages by immunofluorescence on acetone fixed lung cryosections.
Image caption:
Lung macrophages express FcγRI and FcγRII.
Immunofluorescence stainings of lung sections with CD163, Hoechst and FcγRII. Magnification 20x. Scale bars equal 50 μm. Figures are representative of n = 3 lungs from different donors.

From: Bruggeman CW, Houtzager J, Dierdorp B, Kers J, Pals ST, Lutter R, et al. (2019)
Tissue-specific expression of IgG receptors by human macrophages ex vivo.
PLoS ONE 14(10): e0223264.
doi: 10.1371/journal.pone.0223264
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.

Mouse anti Human CD32:Alexa Fluor® 647

Mouse anti Human CD32:Biotin

Mouse anti Human CD32:FITC

Mouse anti Human CD32:Low Endotoxin

Mouse anti Human CD32

Mouse anti Human CD32:RPE

Product Type
Monoclonal Antibody
Clone
AT10
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1075A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA1075A647T
Datasheet
F
SDS
25 Tests/0.25ml loader

MCA1075B
Datasheet
F
SDS
0.1 mg loader

MCA1075BT
Datasheet
F
SDS
25 µg loader

MCA1075F
Datasheet
F
SDS
0.1 mg loader

MCA1075FT
Datasheet
F
SDS
25 µg loader

MCA1075EL
Datasheet
C* F FN IP
SDS
0.5 mg loader

MCA1075
Datasheet
C* F FN* IP
SDS
0.2 mg loader

MCA1075T
Datasheet
C* F IP
SDS
25 µg loader

MCA1075PE
Datasheet
F
SDS
100 Tests loader

MCA1075PET
Datasheet
F
SDS
25 Tests loader

Mouse anti Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.

Mouse anti Human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII (Larsson et al. 1997).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Rhesus Monkey
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor®647- liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 100 Tests
Reconstitute with 1.0 ml distilled water
Pack Size: 25 Tests
Reconstitute with 0.25ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
TRIS buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
Pack Size: 0.2 mg
0.09% Sodium Azide (NaN3)
Pack Size: 25 µg
0.09%Sodium Azide
Preservative Stabilisers
Pack Size: 100 Tests
0.09%Sodium Azide
1%Bovine Serum Albumin
Pack Size: 25 Tests
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
K562 cell line.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 25 Tests
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P12318
P31994
P31995
Entrez Gene
FCGR2A
FCGR2B
FCGR2C
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0019864 IgG binding
GO:0006955 immune response
GO:0044419 interspecies interaction between organisms
GO:0050776 regulation of immune response
GO:0004888 transmembrane receptor activity
GO:0005737 cytoplasm
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD32 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Flow Cytometry Neat 1/10
Flow Cytometry 20ug/ml
Functional Assays
Immunohistology - Frozen 1 1/500 1/1000
Immunohistology - Paraffin
Immunoprecipitation 20ug/ml
Flow Cytometry 20ug/ml
Functional Assays 1
Immunohistology - Frozen 2 1/500 1/1000
Immunohistology - Paraffin
Immunoprecipitation 20ug/ml
Flow Cytometry Neat
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
  2. 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Histology Positive Control Tissue
Lymph node
Histology Positive Control Tissue
Lymph node
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control:Low Endotoxin MCA928EL C F 0.5 mg loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Blocking assay

Flow Cytometry

Functional Assays

Product Specific References

Source Reference

  1. Greenman, J. et al. (1991) Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.
    Mol Immunol. 28 (11): 1243-54.

References for CD32 antibody

  1. Van Den Herik-Oudijk, I.E. et al. (1994) Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes.
    J Immunol. 152 (2): 574-85.
  2. Lilliehöök, I. et al. (1998) Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies.
    Vet Immunol Immunopathol. 61 (2-4): 181-93.
  3. Larsson M et al. (1997) Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro.
    Immunology. 90 (1): 138-46.
  4. Mold, C. and Du Clos, T.W. (2006) C-reactive protein increases cytokine responses to Streptococcus pneumoniae through interactions with Fc gamma receptors.
    J Immunol. 176: 7598-604.
  5. Dutertre, C.A. et al. (2008) A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function.
    J Leukoc Biol. 84 (6): 1511-20.
  6. Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells.
    Vet Immunol Immunopathol. 135: 188-98.
  7. Sims, G.P. et al. (2005) Identification and characterization of circulating human transitional B cells.
    Blood. 105: 4390-8.
  8. Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.
    J Exp Med. 203: 1629-35.
  9. Zhao, X.W. et al. (2011) CD47-signal regulatory protein-α (SIRPα) interactions form a barrier for antibody-mediated tumor cell destruction.
    Proc Natl Acad Sci U S A. 108 (45): 18342-7.
  10. Araújo, M.S.et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
    Vet Immunol Immunopathol. 141: 64-75.
  11. Bonnefont-Rebeix, C. et al. (2006) CD86 molecule is a specific marker for canine monocyte-derived dendritic cells.
    Vet Immunol Immunopathol. 109 (1-2): 167-76.
  12. Santer, D.M. et al. (2010) C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes.
    J Immunol. 185: 4738-49.
  13. Shannon, O. et al. (2010) Platelet activation and biofilm formation by Aerococcus urinae, an endocarditis-causing pathogen.
    Infect Immun. 78: 4268-75.
  14. Ito, T. et al. (1999) A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells.
    J Immunol. 163: 1409-19.
  15. Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
    Vet Parasitol. 220: 33-45.
  16. Gazendam, R.P. et al. (2016) Impaired killing of Candida albicans by granulocytes mobilized for transfusion purposes: a role for granule components.
    Haematologica. 101 (5): 587-96.
  17. Liu M et al. (2011) Vitellogenin mediates phagocytosis through interaction with FcγR.
    Mol Immunol. 49 (1-2): 211-8.
  18. Petersson, F. et al. (2018) Platelet activation and aggregation by the opportunistic pathogen Cutibacterium (Propionibacterium) acnes.
    PLoS One. 13 (1): e0192051.
  19. Kahn, F. et al. (2008) Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response.
    PLoS Pathog. 4 (9): e1000149.
  20. Bruggeman, C.W. et al. (2019) Tissue-specific expression of IgG receptors by human macrophages ex vivo.
    PLoS One. 14 (10): e0223264.
  21. Chen, T. et al. (2020) Capsular glycan recognition provides antibody-mediated immunity against tuberculosis.
    J Clin Invest. 130 (4): 1808-22.

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