CD32 antibody | AT10
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used for blocking of Fc receptor and inhibition of IC-mediated α ifn production by pDSs
Image caption:
The Fc region of 24F4A enhances the inhibition of immune complex‐induced IFNα production by human pDCs A–F Isolated human pDCs were treated with increasing concentrations of 24F4A (red circles), 24F4A‐ef (black squares), or 10 μg/ml of the isotype control (black triangle) and stimulated with 5 μM of R848 (A and B), 1 μM of CpG‐A (C and D), or immune complexes (E and F) for 16 h at 37°C. IFNα concentrations in cultured supernatants were determined using ELISA. Representative plots for each stimulation (A, C and E) and the average IC50 of 24F4A and 24F4A‐ef for each stimulation condition (B, D and F) are shown (n = 3). Horizontal bars represent mean IC50, and error bars represent SD of the mean from three independent experiments. G. Isolated human pDCs were treated with 10 μg/ml of 24F4A (black line), 24F4A‐ef (gray shaded histogram), or the isotype control (gray dotted line) for 10 min at 37°C. Flow cytometry was used to evaluate pSYK and pPLCγ2. Shown is a representative plot of two independent experiments. H. Isolated pDCs treated with 10 μg/ml of anti‐CD32a (AT10) and stimulated with CpG‐A, R848, or immune complexes. Error bars represent SD of percent inhibition of IFNα from three independent experiments.
From: Pellerin A, Otero K, Czerkowicz JM, Kerns HM, Shapiro RI, Ranger AM, Otipoby KL, Taylor FR, Cameron TO, Viney JL, Rabah D.
Anti-BDCA2 monoclonal antibody inhibits plasmacytoid dendritic cell activation through Fc-dependent and Fc-independent mechanisms.
EMBO Mol Med. 2015 Mar 11. pii: e201404719.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used as a blocking agent to assess the effect of FcγRs on heparin binding protein (HBP) release by ELISA.
Image caption:
Antibodies against FcγRII inhibit HBP release.
Whole blood (diluted 1∶10) was incubated with antibodies against different FcγRs, isotype control antibodies, or buffer alone, for 20 minutes at 37°C. M1 protein was added and the HBP release was measured. Data are expressed as percentage of release without any antibodies added, and are the mean±SD from three different individuals, each done in duplicate. The different treatment groups were compared using ANOVA, and when found significantly different the treatment groups were compared against the control group using Dunnett's 2 sided test.
From: Kahn F, Mörgelin M, Shannon O, Norrby-Teglund A, Herwald H, Olin AI, et al. (2008)
Antibodies against a Surface Protein of Streptococcus pyogenes Promote a Pathological Inflammatory Response. PLoS Pathog 4(9): e1000149.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used to evaluate FcγRII (CD32) expression on Kupffer cells by immunofluorescence on acetone fixed cryosections.
Image caption:
Immunofluorescence stainings of liver sections with CD163 and FcγRII. Magnification 20x. Scale bars equal 50 μm. Figures are representative of n = 2 livers from different donors.
From: Bruggeman CW, Houtzager J, Dierdorp B, Kers J, Pals ST, Lutter R, et al. (2019)
Tissue-specific expression of IgG receptors by human macrophages ex vivo.
PLoS ONE 14(10): e0223264.
doi: 10.1371/journal.pone.0223264
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used to evaluate FcγRII (CD32) expression on alveolar macrophages by immunofluorescence on acetone fixed lung cryosections.
Image caption:
Lung macrophages express FcγRI and FcγRII.
Immunofluorescence stainings of lung sections with CD163, Hoechst and FcγRII. Magnification 20x. Scale bars equal 50 μm. Figures are representative of n = 3 lungs from different donors.
From: Bruggeman CW, Houtzager J, Dierdorp B, Kers J, Pals ST, Lutter R, et al. (2019)
Tissue-specific expression of IgG receptors by human macrophages ex vivo.
PLoS ONE 14(10): e0223264.
doi: 10.1371/journal.pone.0223264
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD32:Alexa Fluor® 647
Mouse anti Human CD32:Biotin
Mouse anti Human CD32:FITC
Mouse anti Human CD32:Low Endotoxin
Mouse anti Human CD32
Mouse anti Human CD32:RPE
- Product Type
- Monoclonal Antibody
- Clone
- AT10
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA1075A647T | F | 25 Tests/0.25ml |
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| MCA1075A647 | F | 100 Tests/1ml |
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| MCA1075BT | F | 25 µg |
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| MCA1075B | F | 0.1 mg |
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| MCA1075FT | F | 25 µg |
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| MCA1075F | F | 0.1 mg |
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| MCA1075EL | C* F FN IP | 0.5 mg |
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| MCA1075T | C* F IP | 25 µg |
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| MCA1075 | C* F FN* IP | 0.2 mg |
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| MCA1075PET | F | 25 Tests |
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| MCA1075PE | F | 100 Tests |
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Mouse anti Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.
Mouse anti Human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII (Larsson et al. 1997).
Mouse anti Human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII (Larsson et al. 1997).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Rhesus Monkey Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor®647- liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Pack Size: 25 TestsReconstitute with 0.25ml distilled waterPack Size: 100 TestsReconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
- Pack Size: 25 µg
0.09% Sodium Azide Pack Size: 0.2 mg0.09% Sodium Azide (NaN3) - Preservative Stabilisers
- Pack Size: 25 Tests0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% SucrosePack Size: 100 Tests0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- K562 cell line.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 TestsPrior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P12318
- P31994
- P31995
- Entrez Gene
- FCGR2A
- FCGR2B
- FCGR2C
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0019864 IgG binding
- GO:0006955 immune response
- GO:0044419 interspecies interaction between organisms
- GO:0050776 regulation of immune response
- GO:0004888 transmembrane receptor activity
- GO:0005737 cytoplasm
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD32 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 20ug/ml | ||
| Functional Assays | |||
| Immunohistology - Frozen 1 | 1/500 | 1/1000 | |
| Immunohistology - Paraffin | |||
| Immunoprecipitation | 20ug/ml | ||
| Flow Cytometry | 20ug/ml | ||
| Functional Assays 1 | |||
| Immunohistology - Frozen 2 | 1/500 | 1/1000 | |
| Immunohistology - Paraffin | |||
| Immunoprecipitation | 20ug/ml | ||
| Flow Cytometry | Neat |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.
- 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Histology Positive Control Tissue
- Lymph node
- Histology Positive Control Tissue
- Lymph node
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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| Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | C F | 0.5 mg |
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| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
Source Reference
-
Greenman, J. et al. (1991) Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.
Mol Immunol. 28 (11): 1243-54.
References for CD32 antibody
-
Van Den Herik-Oudijk, I.E. et al. (1994) Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes.
J Immunol. 152 (2): 574-85. -
Lilliehöök, I. et al. (1998) Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies.
Vet Immunol Immunopathol. 61 (2-4): 181-93. -
Larsson M et al. (1997) Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro.
Immunology. 90 (1): 138-46. -
Dutertre, C.A. et al. (2008) A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function.
J Leukoc Biol. 84 (6): 1511-20. -
Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells.
Vet Immunol Immunopathol. 135: 188-98. -
Sims, G.P. et al. (2005) Identification and characterization of circulating human transitional B cells.
Blood. 105: 4390-8. -
Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.
J Exp Med. 203: 1629-35. -
Zhao, X.W. et al. (2011) CD47-signal regulatory protein-α (SIRPα) interactions form a barrier for antibody-mediated tumor cell destruction.
Proc Natl Acad Sci U S A. 108 (45): 18342-7. -
Araújo, M.S.et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
Vet Immunol Immunopathol. 141: 64-75. -
Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.
J Exp Med. 203: 1629-35. -
Santer, D.M. et al. (2010) C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes.
J Immunol. 185: 4738-49. -
Shannon, O. et al. (2010) Platelet activation and biofilm formation by Aerococcus urinae, an endocarditis-causing pathogen.
Infect Immun. 78: 4268-75. -
Ito, T. et al. (1999) A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells.
J Immunol. 163: 1409-19. -
Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
Vet Parasitol. 220: 33-45. -
Gazendam, R.P. et al. (2016) Impaired killing of Candida albicans by granulocytes mobilized for transfusion purposes: a role for granule components.
Haematologica. 101 (5): 587-96. -
Liu M et al. (2011) Vitellogenin mediates phagocytosis through interaction with FcγR.
Mol Immunol. 49 (1-2): 211-8. -
Petersson, F. et al. (2018) Platelet activation and aggregation by the opportunistic pathogen Cutibacterium (Propionibacterium) acnes.
PLoS One. 13 (1): e0192051. -
Kahn, F. et al. (2008) Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response.
PLoS Pathog. 4 (9): e1000149. -
Bruggeman, C.W. et al. (2019) Tissue-specific expression of IgG receptors by human macrophages ex vivo.
PLoS One. 14 (10): e0223264.
