CD32 antibody | AT10
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|Mouse anti Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.
Mouse anti Human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII (Larsson et al. 1997).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Dog Rhesus Monkey Pig
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid.
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- 0.09% sodium azide (NaN3).
- K562 cell line.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml.
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- For research purposes only.
- 12 months from date of despatch.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Functional Assays 1|
|Immunohistology - Frozen 2||1/500||1/1000|
|Immunohistology - Paraffin|
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or cells or 100μl whole blood.
- Histology Positive Control Tissue
- Lymph node.
Greenman, J. et al. (1991) Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.
Mol Immunol. 28 (11): 1243-54.
References for CD32 antibody
Pawar, P. et al. (2004) Fluid shear regulates the kinetics and receptor specificity of Staphylococcus aureus binding to activated platelets.
J Immunol. 173: 1258-65.
Su, K. et al. (2004) A promoter haplotype of the immunoreceptor tyrosine-based inhibitory motif-bearing FcgammaRIIb alters receptor expression and associates with autoimmunity. II. Differential binding of GATA4 and Yin-Yang1 transcription factors and correlated receptor expression and function.
J Immunol. 172: 7192-9.
van der Heijden, J. et al. (2012) Phenotypic variation in IgG receptors by nonclassical FCGR2C alleles.
J Immunol. 188 (3): 1318-24.
Pellerin, A. et al. (2015) Anti-BDCA2 monoclonal antibody inhibits plasmacytoid dendritic cell activation through Fc-dependent and Fc-independent mechanisms.
EMBO Mol Med. 7 (4): 464-76.
Van Den Herik-Oudijk, I.E. et al. (1994) Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes.
J Immunol. 152 (2): 574-85.
Lilliehöök, I. et al. (1998) Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies.
Vet Immunol Immunopathol. 61 (2-4): 181-93.
Larsson M et al. (1997) Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro.
Immunology. 90 (1): 138-46.
Mold, C. and Du Clos, T.W. (2006) C-reactive protein increases cytokine responses to Streptococcus pneumoniae through interactions with Fc gamma receptors.
J Immunol. 176: 7598-604.
View The Latest Product References
Dutertre, C.A. et al. (2008) A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function.
J Leukoc Biol. 84 (6): 1511-20.
Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells.
Vet Immunol Immunopathol. 135: 188-98.
Sims, G.P. et al. (2005) Identification and characterization of circulating human transitional B cells.
Blood. 105: 4390-8.
Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.
J Exp Med. 203: 1629-35.
Zhao, X.W. et al. (2011) CD47-signal regulatory protein-α (SIRPα) interactions form a barrier for antibody-mediated tumor cell destruction.
Proc Natl Acad Sci U S A. 108 (45): 18342-7.
Araújo, M.S.et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
Vet Immunol Immunopathol. 141: 64-75.
Bonnefont-Rebeix, C. et al. (2006) CD86 molecule is a specific marker for canine monocyte-derived dendritic cells.
Vet Immunol Immunopathol. 109 (1-2): 167-76.
Santer, D.M. et al. (2010) C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes.
J Immunol. 185: 4738-49.
Shannon, O. et al. (2010) Platelet activation and biofilm formation by Aerococcus urinae, an endocarditis-causing pathogen.
Infect Immun. 78: 4268-75.
Ito, T. et al. (1999) A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells.
J Immunol. 163: 1409-19.
Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
Vet Parasitol. 220: 33-45.
Gazendam, R.P. et al. (2016) Impaired killing of Candida albicans by granulocytes mobilized for transfusion purposes: a role for granule components.
Haematologica. 101 (5): 587-96.
Liu M et al. (2011) Vitellogenin mediates phagocytosis through interaction with FcγR.
Mol Immunol. 49 (1-2): 211-8.
Petersson, F. et al. (2018) Platelet activation and aggregation by the opportunistic pathogen Cutibacterium (Propionibacterium) acnes.
PLoS One. 13 (1): e0192051.
Kahn, F. et al. (2008) Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response.
PLoS Pathog. 4 (9): e1000149.
Bruggeman, C.W. et al. (2019) Tissue-specific expression of IgG receptors by human macrophages ex vivo.
PLoS One. 14 (10): e0223264.
Chen, T. et al. (2020) Capsular glycan recognition provides antibody-mediated immunity against tuberculosis.
J Clin Invest. 130 (4): 1808-22.
Teuben, M.P.J. et al. (2021) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.
Eur J Trauma Emerg Surg. Jun 10 [Epub ahead of print].
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0019864 IgG binding
- GO:0006955 immune response
- GO:0044419 interspecies interaction between organisms
- GO:0050776 regulation of immune response
- GO:0004888 transmembrane receptor activity
- GO:0005737 cytoplasm
- View More GO Terms
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