CD32 antibody | AT10
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used for blocking of Fc receptor and inhibition of IC-mediated α ifn production by pDSs
Image caption:
The Fc region of 24F4A enhances the inhibition of immune complex‐induced IFNα production by human pDCs A–F Isolated human pDCs were treated with increasing concentrations of 24F4A (red circles), 24F4A‐ef (black squares), or 10 μg/ml of the isotype control (black triangle) and stimulated with 5 μM of R848 (A and B), 1 μM of CpG‐A (C and D), or immune complexes (E and F) for 16 h at 37°C. IFNα concentrations in cultured supernatants were determined using ELISA. Representative plots for each stimulation (A, C and E) and the average IC50 of 24F4A and 24F4A‐ef for each stimulation condition (B, D and F) are shown (n = 3). Horizontal bars represent mean IC50, and error bars represent SD of the mean from three independent experiments. G. Isolated human pDCs were treated with 10 μg/ml of 24F4A (black line), 24F4A‐ef (gray shaded histogram), or the isotype control (gray dotted line) for 10 min at 37°C. Flow cytometry was used to evaluate pSYK and pPLCγ2. Shown is a representative plot of two independent experiments. H. Isolated pDCs treated with 10 μg/ml of anti‐CD32a (AT10) and stimulated with CpG‐A, R848, or immune complexes. Error bars represent SD of percent inhibition of IFNα from three independent experiments.
From: Pellerin A, Otero K, Czerkowicz JM, Kerns HM, Shapiro RI, Ranger AM, Otipoby KL, Taylor FR, Cameron TO, Viney JL, Rabah D.
Anti-BDCA2 monoclonal antibody inhibits plasmacytoid dendritic cell activation through Fc-dependent and Fc-independent mechanisms.
EMBO Mol Med. 2015 Mar 11. pii: e201404719.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used as a blocking agent to assess the effect of FcγRs on heparin binding protein (HBP) release by ELISA.
Image caption:
Antibodies against FcγRII inhibit HBP release.
Whole blood (diluted 1∶10) was incubated with antibodies against different FcγRs, isotype control antibodies, or buffer alone, for 20 minutes at 37°C. M1 protein was added and the HBP release was measured. Data are expressed as percentage of release without any antibodies added, and are the mean±SD from three different individuals, each done in duplicate. The different treatment groups were compared using ANOVA, and when found significantly different the treatment groups were compared against the control group using Dunnett's 2 sided test.
From: Kahn F, Mörgelin M, Shannon O, Norrby-Teglund A, Herwald H, Olin AI, et al. (2008)
Antibodies against a Surface Protein of Streptococcus pyogenes Promote a Pathological Inflammatory Response. PLoS Pathog 4(9): e1000149.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used to evaluate FcγRII (CD32) expression on alveolar macrophages by immunofluorescence on acetone fixed lung cryosections.
Image caption:
Lung macrophages express FcγRI and FcγRII.
Immunofluorescence stainings of lung sections with CD163, Hoechst and FcγRII. Magnification 20x. Scale bars equal 50 μm. Figures are representative of n = 3 lungs from different donors.
From: Bruggeman CW, Houtzager J, Dierdorp B, Kers J, Pals ST, Lutter R, et al. (2019)
Tissue-specific expression of IgG receptors by human macrophages ex vivo.
PLoS ONE 14(10): e0223264.
doi: 10.1371/journal.pone.0223264
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD32 antibody, clone AT10 (MCA1075) used to evaluate FcγRII (CD32) expression on Kupffer cells by immunofluorescence on acetone fixed cryosections.
Image caption:
Immunofluorescence stainings of liver sections with CD163 and FcγRII. Magnification 20x. Scale bars equal 50 μm. Figures are representative of n = 2 livers from different donors.
From: Bruggeman CW, Houtzager J, Dierdorp B, Kers J, Pals ST, Lutter R, et al. (2019)
Tissue-specific expression of IgG receptors by human macrophages ex vivo.
PLoS ONE 14(10): e0223264.
doi: 10.1371/journal.pone.0223264
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD32
- Product Type
- Monoclonal Antibody
- Clone
- AT10
- Isotype
- IgG1
- Specificity
- CD32
Antibody Quality and Performance Guarantee
| Mouse anti Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Mouse anti Human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII (Larsson et al. 1997). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Rhesus Monkey Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- K562 cell line.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
20ug/ml | |
| Functional Assays 1 | |
||
| Immunohistology - Frozen 2 | |
1/500 | 1/1000 |
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |
20ug/ml |
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or cells or 100μl whole blood
- Histology Positive Control Tissue
- Lymph node
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Greenman, J. et al. (1991) Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.
Mol Immunol. 28 (11): 1243-54.
References for CD32 antibody
-
Van Den Herik-Oudijk, I.E. et al. (1994) Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes.
J Immunol. 152 (2): 574-85. -
Larsson M et al. (1997) Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro.
Immunology. 90 (1): 138-46. -
Lilliehöök, I. et al. (1998) Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies.
Vet Immunol Immunopathol. 61 (2-4): 181-93. -
Ito, T. et al. (1999) A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells.
J Immunol. 163: 1409-19. -
Su, K. et al. (2004) A promoter haplotype of the immunoreceptor tyrosine-based inhibitory motif-bearing FcgammaRIIb alters receptor expression and associates with autoimmunity. II. Differential binding of GATA4 and Yin-Yang1 transcription factors and correlated receptor expression and function.
J Immunol. 172: 7192-9. -
Pawar, P. et al. (2004) Fluid shear regulates the kinetics and receptor specificity of Staphylococcus aureus binding to activated platelets.
J Immunol. 173: 1258-65. -
Sims, G.P. et al. (2005) Identification and characterization of circulating human transitional B cells.
Blood. 105: 4390-8. -
Bonnefont-Rebeix, C. et al. (2006) CD86 molecule is a specific marker for canine monocyte-derived dendritic cells.
Vet Immunol Immunopathol. 109 (1-2): 167-76.
View The Latest Product References
-
Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.
J Exp Med. 203: 1629-35. -
Mold, C. and Du Clos, T.W. (2006) C-reactive protein increases cytokine responses to Streptococcus pneumoniae through interactions with Fc gamma receptors.
J Immunol. 176: 7598-604. -
Dutertre, C.A. et al. (2008) A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function.
J Leukoc Biol. 84 (6): 1511-20. -
Kahn, F. et al. (2008) Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response.
PLoS Pathog. 4 (9): e1000149. -
Shannon, O. et al. (2010) Platelet activation and biofilm formation by Aerococcus urinae, an endocarditis-causing pathogen.
Infect Immun. 78: 4268-75. -
Santer, D.M. et al. (2010) C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes.
J Immunol. 185: 4738-49. -
Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells.
Vet Immunol Immunopathol. 135: 188-98. -
Araújo, M.S.et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
Vet Immunol Immunopathol. 141: 64-75. -
Liu M et al. (2011) Vitellogenin mediates phagocytosis through interaction with FcγR.
Mol Immunol. 49 (1-2): 211-8. -
Zhao, X.W. et al. (2011) CD47-signal regulatory protein-α (SIRPα) interactions form a barrier for antibody-mediated tumor cell destruction.
Proc Natl Acad Sci U S A. 108 (45): 18342-7. -
van der Heijden, J. et al. (2012) Phenotypic variation in IgG receptors by nonclassical FCGR2C alleles.
J Immunol. 188 (3): 1318-24. -
Pellerin, A. et al. (2015) Anti-BDCA2 monoclonal antibody inhibits plasmacytoid dendritic cell activation through Fc-dependent and Fc-independent mechanisms.
EMBO Mol Med. 7 (4): 464-76. -
Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
Vet Parasitol. 220: 33-45. -
Gazendam, R.P. et al. (2016) Impaired killing of Candida albicans by granulocytes mobilized for transfusion purposes: a role for granule components.
Haematologica. 101 (5): 587-96. -
Petersson, F. et al. (2018) Platelet activation and aggregation by the opportunistic pathogen Cutibacterium (Propionibacterium) acnes.
PLoS One. 13 (1): e0192051. -
Bruggeman, C.W. et al. (2019) Tissue-specific expression of IgG receptors by human macrophages ex vivo.
PLoS One. 14 (10): e0223264. -
Chen, T. et al. (2020) Capsular glycan recognition provides antibody-mediated immunity against tuberculosis.
J Clin Invest. 130 (4): 1808-22. -
Teuben, M.P.J. et al. (2021) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.
Eur J Trauma Emerg Surg. Jun 10 [Epub ahead of print]. -
Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
Cells. 12 (16): 2103. -
Jimenez, R.V. et al. (2018) C-Reactive Protein Impairs Dendritic Cell Development, Maturation, and Function: Implications for Peripheral Tolerance.
Front Immunol. 9: 372. -
Prat, M. et al. (2021) Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation.
Cancers (Basel). 13 (8): 1845
- Synonyms
- FcRII
- RRID
- AB_321659
- UniProt
- P12318
- P31994
- P31995
- Entrez Gene
- FCGR2A
- FCGR2B
- FCGR2C
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0019864 IgG binding
- GO:0006955 immune response
- GO:0044419 interspecies interaction between organisms
- GO:0050776 regulation of immune response
- GO:0004888 transmembrane receptor activity
- GO:0005737 cytoplasm
- View More GO Terms
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