CD3 antibody | SK7

Mouse anti Human CD3

Product Type
Monoclonal Antibody
Clone
SK7
Isotype
IgG1
Specificity
CD3

Product Code Applications Pack Size List Price Your Price Qty
MCA6147GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD3 antibody, clone SK7 recognizes the T-cell surface glycoprotein CD3 epsilon chain (CD3ɛ), also known as T-cell surface antigen T3/Leu-4 epsilon. CD3ɛ is a subunit of the TCR-CD3 cell surface receptor complex, which is expressed at the surface of mature T cells, NKT cells and thymocytes (Mariuzza et al. 2020). The CD3 complex is required for intracellular signaling and for TCR surface expression. Recruitment of the TCR/CD3 complex initiates early events leading to proliferation and differentiation of mature T cells into an effector cell (Guy & Vignali 2009).

Deficiency of the CD3ε chain contributes to blocking T-cell development and presentation of a severe combined immunodeficiency (SCID) phenotype (Fischer et al. 2005).

Mouse anti-human CD3, clone SK7 binding to CD3 has been shown to be blocked by clone OKT3 and 12F6 (Li et al. 2005).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human CD3ɛ
Purity
>90 % by SDS PAGE
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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UniProt
P07766
Entrez Gene
CD3E
GO Terms
GO:0004888 transmembrane receptor activity
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0031295 T cell costimulation
GO:0042608 T cell receptor binding
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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