CD3 antibody | MEM-57

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Mouse anti Human CD3:FITC

CD3 antibody, clone MEM-57, recognizes an extracellular epitope of the human CD3 complex which is expressed on the surface of circulating human peripheral T cells, thymocytes, Purkinji cells and neurons present in the cerebellar cortex.

Mouse anti Human CD3

CD3 antibody, clone MEM-57, recognizes an extracellular epitope of the human CD3 complex which is expressed on the surface of circulating human peripheral T cells, thymocytes, Purkinji cells and neurons present in the cerebellar cortex.

Mouse anti Human CD3:RPE

CD3 antibody, clone MEM-57, recognizes an extracellular epitope of the human CD3 complex which is expressed on the surface of circulating human peripheral T cells, thymocytes, Purkinji cells and neurons present in the cerebellar cortex.

Product Type
Monoclonal Antibody
Clone
MEM-57
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2184FT F 25 µg
MCA2184F F 0.1 mg
MCA2184 F IP 0.2 mg
MCA2184PE F 100 Tests
Mouse anti Human CD3 (MEM-57) is a monoclonal antibody recognizing an extracellular epitope of the human CD3 complex containing either δ-ε or γ-ε subunit complexes (Dave et al. 1997). Mouse anti Human CD3 (MEM-57) recognizes a conformational CD3 epitope minimally requiring the presence of the CD3ε subunit (Transy et al. 1989) as it will recognize murine 3D054.8 hybridoma cells tranfected with cDNA to express the human CDε subunit lacking its cytoplasmic domain. CD3 serves as a pan T cell marker, it is expressed on the surface of over 95% of circulating human peripheral T cells. CD3 is also present on ~60-80% of thymocytes and on Purkinji cells, neurons present in the cerebellar cortex. CD3 is not expressed on B cells or NK cells.

The TCR complex consists of TCR heterodimers, either α/β or γ/δ expressed in a mutually exclusive manner, analagous that seen in mice on different T cell lineages. In order for the TCR heterodimer to be expressed on the cell surface it must associate with a minimal CD3 complex. The CD3 complex itself is made up of four subunits, γ, δ, ε and ζ responsible for effecitng signalling upon TCR activation, via a single or multiple, depending on the subunit, immunoreceptor tyrosine-based activation motif (ITAM) present in the CD3 subunit cytoplasmic domain (Kuhns et al. 2006 Dave et al. 1997).

Mouse anti Human CD3 clone MEM-57 demonstrates a mitogenic effect on peripheral blood T cells and on the lymphoblastoid Jurkat cell line (Batista et al. 2004 Brdicková et al. 2003). Clone MEM-57 immunoprecipitates proteins of ~22 kDa and more weakly ~28 kDa. from peripheral blood T cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Purity
>95% by SDS-PAGE
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P04234 Related reagents
P07766 Related reagents
P09693 Related reagents
Entrez Gene
CD3D Related reagents
CD3E Related reagents
CD3G Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005737 cytoplasm
GO:0031295 T cell costimulation
GO:0045059 positive thymic T cell selection
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0042608 T cell receptor binding
GO:0006461 protein complex assembly
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0042110 T cell activation
Regulatory
For research purposes only

Applications of CD3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10
Flow Cytometry 1/5 1/2
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F 100 Tests F
Mouse IgG2a Negative Control MCA929 100 Tests F
Mouse IgG2a Negative Control:RPE MCA929PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD3 antibody

  1. Horejsí, V. et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
    Folia Biol (Praha). 34 (1): 23-34.
  2. Dave, V.P. et al. (1998) Altered functional responsiveness of thymocyte subsets from CD3delta-deficient mice to TCR-CD3 engagement.
    Int Immunol. 10: 1481-90.
  3. Transy, C. et al. (1989) Most anti-human CD3 monoclonal antibodies are directed to the CD3 epsilon subunit.
    Eur J Immunol. 19: 947-50.
  4. Amlot, P.L. et al. (1996) Activation antigen expression on human T cells. I. Analysis by two-colour flow cytometry of umbilical cord blood, adult blood and lymphoid tissue.
    Clin Exp Immunol. 105: 176-82.
  5. Gunnlaugsdottir, B. et al. (2008) Naive human T-cells become non-responsive towards anti-TNFalpha (infliximab) treatment in vitro if co-stimulated through CD28.
    Scand J Immunol. 68: 624-34.
  6. Morris, R.J. et al. (2005) A high-efficiency system of natural killer cell cloning.
    J Immunol Methods. 307: 24-33.
  7. Bughani U et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
    PLoS One. 12 (7): e0180088.

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