CD274 antibody | 29E.2A3

Mouse anti Human CD274

Product Type
Monoclonal Antibody
Clone
29E.2A3
Isotype
IgG2b
Specificity
CD274

Product Code Applications Pack Size List Price Your Price Qty
MCA6132
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F FN 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD274 antibody, clone 29E.2A3 detects human CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T cells, B cells, endothelial cells and epithelial cells in response to interferons alpha, beta and gamma.

CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naïve T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to co-stimulate early T cell priming and differentiation.

Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Full length human CD274
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Functional Assays
Immunohistology - Frozen 1
  1. 1Acetone fixation is recommended
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Synonyms
PD-L1
UniProt
Q9NZQ7
Entrez Gene
CD274
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0006955 immune response
GO:0007166 cell surface receptor linked signaling pathway
GO:0008283 cell proliferation
GO:0012505 endomembrane system
GO:0031295 T cell costimulation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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