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CD27 antibody | LT27

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Anti Human CD27 Antibody, clone LT27 thumbnail image 1 Anti Human CD27 Antibody, clone LT27 thumbnail image 2
Anti Human CD27 Antibody, clone LT27 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD19 (MCA1940F) and A647 conjugated Mouse IgG2a isotype control (MCA929A647). Figure B FITC conjugated Mouse anti Human CD19 (MCA1940F) and A647 conjugated Mouse anti Human CD27 (MCA755A647). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Anti Human CD27 Antibody, clone LT27 gallery image 2
Published customer image:
Mouse anti Human CD27 antibody, clone LT27 (MCA755GA) used for the evaluation of CD27 expression on human B cell subsets by flow cytometry.
Image caption:
Memory B cell sorting strategies.
Subsets of human B cells (CD45+CD19+) were sorted from BM and spleens of vaccinated HIS mice. The strategy 1 (upper part) was used on 3 responder HBsAg-vaccinated animals and gave rise to the following B cell subsets: (i) CD27hiCD38hi, (ii) CD27+CD38lo/intIgD+, and (iii) CD27+CD38lo/intIgD−. The strategy 2 (lower part) was used on 3 responder TT-vaccinated animals and gave rise to the following B cell subsets: (iv) CD27+IgM+IgG−, and (v) CD27+IgM−IgG+. The respective frequency and name (from i to v) of each sorted B cell subsets is indicated in the corresponding flow cytometry dot plot.).

From: Becker PD, Legrand N, van Geelen CMM, Noerder M, Huntington ND, Lim A, et al. (2010)
Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated “Human Immune System” Mice.
PLoS ONE 5(10): e13137.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD27:Alexa Fluor® 647

Mouse anti Human CD27:Preservative Free

Mouse anti Human CD27:FITC

Mouse anti Human CD27

Mouse anti Human CD27:RPE

Product Type
Monoclonal Antibody
Clone
LT27
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA755A647T
Datasheet
F
MSDS
25 Tests/0.25ml loader

MCA755A647
Datasheet
F
MSDS
100 Tests/1ml loader

MCA755XZ
Datasheet
F IP
MSDS
1 mg loader

MCA755F
Datasheet
F
MSDS
0.1 mg loader

MCA755GA
Datasheet
F IP
MSDS
0.1 mg loader

MCA755PET
Datasheet
F
MSDS
25 Tests/0.25ml loader

MCA755PE
Datasheet
F
MSDS
100 Tests loader

Mouse anti human CD27, clone LT27, recognizes the human T-cell activation antigen, CD27. This antigen is expressed on activated T lymphocytes and belongs to a family of proteins associated with the nerve growth factor receptor. CD27 is expressed by thymocytes and peripheral T cells and also by EBV transformed B cell lines.

Mouse anti human CD27, clone LT27 precipitates a ~55 kDa protein under reducing conditions or a ~120 kDa protein under non-reducing conditions.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Pack Size: 25 Tests/0.25ml
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Pack Size: 100 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 25 Tests/0.25ml
Reconstitute with 0.25 ml distilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by ion exchange chromatography
Preparation
Purified IgG prepared by ion exchange chromatography
Preparation
Purified IgG prepared by ion exchange chromatography
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1.0%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
1.0%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
Pack Size: 25 Tests/0.25ml
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Pack Size: 100 Tests
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Normal human blood lymphocytes.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 25 Tests/0.25ml
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch
Guarantee
12 months from date of reconstitution.

More Information

UniProt
P26842
Entrez Gene
CD27
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005576 extracellular region
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0006916 anti-apoptosis
GO:0006917 induction of apoptosis
GO:0007166 cell surface receptor linked signaling pathway
GO:0008588 release of cytoplasmic sequestered NF-kappaB
GO:0016064 immunoglobulin mediated immune response
GO:0043027 caspase inhibitor activity
GO:0045579 positive regulation of B cell differentiation
GO:0046330 positive regulation of JNK cascade
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD27 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/100
Immunoprecipitation
Flow Cytometry Neat
Flow Cytometry 1/50 1/100
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA929A647 F 100 Tests/1ml loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Product Specific References

References for CD27 antibody

  1. Ravoet, A.M. et al. (1989) CD25 mAb: epitopes recognised, effect on lymphocyte activation, mediation of ADCC
    in Leucocyte Typing IV: White Cell Differentiation Antigens Eds Knapp, W. et al. Oxford University Press pp 408-411.
  2. Boxall, S. et al. (2004) Abnormal cell surface antigen expression in individuals with variant CD45 splicing and histiocytosis.
    Pediatr Res. 55 (3): 478-84.
  3. Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
    Vet Immunol Immunopathol. 119: 115-22.
  4. Becker, P.D. et al. (2010) Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated ‘‘Human Immune System’’ Mice
    PLoS One. 5(10). pii: e13137.
  5. van Zelm, M.C. et al. (2007) Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion.
    J Exp Med. 204: 645-55.
  6. Steger, B. et al. (2014) CD4(+)and CD8(+)T-cell reactions against leukemia-associated- or minor-histocompatibility-antigens in AML-patients after allogeneic SCT.
    Immunobiology. 219 (4): 247-60.
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