CD27 antibody | LT27

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Anti Human CD27 Antibody, clone LT27 thumbnail image 1

Anti Human CD27 Antibody, clone LT27 thumbnail image 2

Anti Human CD27 Antibody, clone LT27 gallery image 1 — Figure A. FITC conjugated Mouse anti Human CD19 (MCA1940F) and A647 conjugated Mouse IgG2a isotype control (MCA929A647). Figure B FITC conjugated Mouse anti Human CD19 (MCA1940F) and A647 conjugated Mouse anti Human CD27 (**MCA755A647**). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Anti Human CD27 Antibody, clone LT27 gallery image 2 — **Published customer image:**
Mouse anti Human CD27 antibody, clone LT27 (**MCA755GA**) used for the evaluation of CD27 expression on human B cell subsets by flow cytometry.
**Image caption:**
Memory B cell sorting strategies.
Subsets of human B cells (CD45⁺CD19⁺) were sorted from BM and spleens of vaccinated HIS mice. The strategy 1 (upper part) was used on 3 responder HBsAg-vaccinated animals and gave rise to the following B cell subsets: (i) CD27^hiCD38^hi, (ii) CD27⁺CD38^lo/intIgD⁺, and (iii) CD27⁺CD38^lo/intIgD⁻. The strategy 2 (lower part) was used on 3 responder TT-vaccinated animals and gave rise to the following B cell subsets: (iv) CD27⁺IgM⁺IgG⁻, and (v) CD27⁺IgM⁻IgG⁺. The respective frequency and name (from i to v) of each sorted B cell subsets is indicated in the corresponding flow cytometry dot plot.).

From: Becker PD, Legrand N, van Geelen CMM, Noerder M, Huntington ND, Lim A, *et al*. (2010)
Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated “Human Immune System” Mice.
PLoS ONE 5(10): e13137.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD27:Alexa Fluor® 647

Mouse anti Human CD27:Preservative Free

Mouse anti Human CD27:FITC

Mouse anti Human CD27

Mouse anti Human CD27:RPE

Product Type: Monoclonal Antibody
Clone: LT27
Isotype: IgG2a

Product Code	Applications	Pack Size
MCA755A647T	F	25 Tests/0.25ml
MCA755A647	F	100 Tests/1ml
MCA755XZ	F IP	1 mg
MCA755F	F	0.1 mg
MCA755GA	F IP	0.1 mg
MCA755PET	F	25 Tests/0.25ml
MCA755PE	F	100 Tests

Mouse anti human CD27, clone LT27, recognizes the human T-cell activation antigen, CD27. This antigen is expressed on activated T lymphocytes and belongs to a family of proteins associated with the nerve growth factor receptor. CD27 is expressed by thymocytes and peripheral T cells and also by EBV transformed B cell lines.

Mouse anti human CD27, clone LT27 precipitates a ~55 kDa protein under reducing conditions or a ~120 kDa protein under non-reducing conditions.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Cynomolgus monkey
Rhesus Monkey
Sheep

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Pack Size: 25 Tests/0.25ml

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised

Pack Size: 100 Tests

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Pack Size: 25 Tests/0.25ml

Reconstitute with 0.25 ml distilled water

Pack Size: 100 Tests

Reconstitute with 1 ml distilled water

Preparation

Purified IgG prepared by ion exchange chromatography

Preparation

Purified IgG prepared by ion exchange chromatography

Preparation

Purified IgG prepared by ion exchange chromatography

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1.0%	Bovine Serum Albumin

Preservative Stabilisers

None present

Preservative Stabilisers

0.09%	Sodium Azide
1.0%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

Pack Size: 25 Tests/0.25ml

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Pack Size: 100 Tests

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Immunogen

Normal human blood lymphocytes.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^oC only.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Pack Size: 25 Tests/0.25ml
Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Pack Size: 100 Tests
Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch
Guarantee: 12 months from date of reconstitution.

More Information

UniProt: P26842 Related reagents
Entrez Gene: CD27 Related reagents
GO Terms: GO:0005515 protein binding; GO:0006915 apoptosis; GO:0005576 extracellular region; GO:0004888 transmembrane receptor activity; GO:0005887 integral to plasma membrane; GO:0006916 anti-apoptosis; GO:0006917 induction of apoptosis; GO:0007166 cell surface receptor linked signaling pathway; GO:0008588 release of cytoplasmic sequestered NF-kappaB; GO:0016064 immunoglobulin mediated immune response; GO:0043027 caspase inhibitor activity; GO:0045579 positive regulation of B cell differentiation; GO:0046330 positive regulation of JNK cascade
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD27 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry		1/100
Immunoprecipitation
Flow Cytometry		Neat
Flow Cytometry	1/50	1/100
Immunoprecipitation
Flow Cytometry		Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG2a:FITC	HCA037F	0.1 mg	F
Human anti Mouse IgG2a:HRP	HCA037P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F
Human anti Mouse IgG2a:FITC	HCA037F	0.1 mg	F
Human anti Mouse IgG2a:HRP	HCA037P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG2a Negative Control:Alexa Fluor® 647	MCA929A647	100 Tests/1ml	F
Mouse IgG2a Negative Control	MCA929	100 Tests	F
Mouse IgG2a Negative Control:FITC	MCA929F	100 Tests	F
Mouse IgG2a Negative Control	MCA929	100 Tests	F
Mouse IgG2a Negative Control:RPE	MCA929PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD27 antibody

Ravoet, A.M. et al. (1989) CD25 mAb: epitopes recognised, effect on lymphocyte activation, mediation of ADCC
in Leucocyte Typing IV: White Cell Differentiation Antigens Eds Knapp, W. et al. Oxford University Press pp 408-411.
Boxall, S. et al. (2004) Abnormal cell surface antigen expression in individuals with variant CD45 splicing and histiocytosis.
Pediatr Res. 55 (3): 478-84.
Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
Vet Immunol Immunopathol. 119: 115-22.
Becker, P.D. et al. (2010) Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated ‘‘Human Immune System’’ Mice
PLoS One. 5(10). pii: e13137.
van Zelm, M.C. et al. (2007) Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion.
J Exp Med. 204: 645-55.
Steger, B. et al. (2014) CD4(+)and CD8(+)T-cell reactions against leukemia-associated- or minor-histocompatibility-antigens in AML-patients after allogeneic SCT.
Immunobiology. 219 (4): 247-60.

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