CD27 antibody | LT27
Mouse anti Human CD27 antibody, clone LT27 (MCA755GA) used for the evaluation of CD27 expression on human B cell subsets by flow cytometry.
Image caption:
Memory B cell sorting strategies.
Subsets of human B cells (CD45+CD19+) were sorted from BM and spleens of vaccinated HIS mice. The strategy 1 (upper part) was used on 3 responder HBsAg-vaccinated animals and gave rise to the following B cell subsets: (i) CD27hiCD38hi, (ii) CD27+CD38lo/intIgD+, and (iii) CD27+CD38lo/intIgD−. The strategy 2 (lower part) was used on 3 responder TT-vaccinated animals and gave rise to the following B cell subsets: (iv) CD27+IgM+IgG−, and (v) CD27+IgM−IgG+. The respective frequency and name (from i to v) of each sorted B cell subsets is indicated in the corresponding flow cytometry dot plot.).
From: Becker PD, Legrand N, van Geelen CMM, Noerder M, Huntington ND, Lim A, et al. (2010)
Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated “Human Immune System” Mice.
PLoS ONE 5(10): e13137.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD27:Alexa Fluor® 647
Mouse anti Human CD27:Preservative Free
Mouse anti Human CD27:FITC
Mouse anti Human CD27
Mouse anti Human CD27:RPE
- Product Type
- Monoclonal Antibody
- Clone
- LT27
- Isotype
- IgG2a
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA755A647T | F | 25 Tests/0.25ml |
|
|
| MCA755A647 | F | 100 Tests/1ml |
|
|
| MCA755XZ | F IP | 1 mg |
|
|
| MCA755F | F | 0.1 mg |
|
|
| MCA755GA | F IP | 0.1 mg |
|
|
| MCA755PET | F | 25 Tests/0.25ml |
|
|
| MCA755PE | F | 100 Tests |
|
Mouse anti human CD27, clone LT27, recognizes the human T-cell activation antigen, CD27. This antigen is expressed on activated T lymphocytes and belongs to a family of proteins associated with the nerve growth factor receptor. CD27 is expressed by thymocytes and peripheral T cells and also by EBV transformed B cell lines.
Mouse anti human CD27, clone LT27 precipitates a ~55 kDa protein under reducing conditions or a ~120 kDa protein under non-reducing conditions.
Mouse anti human CD27, clone LT27 precipitates a ~55 kDa protein under reducing conditions or a ~120 kDa protein under non-reducing conditions.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilisedPack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Pack Size: 25 Tests/0.25mlReconstitute with 0.25 ml distilled waterPack Size: 100 TestsReconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1.0% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide 1.0% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- Pack Size: 25 Tests/0.25ml0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% SucrosePack Size: 100 Tests0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Immunogen
- Normal human blood lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 Tests/0.25mlPrior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch
- Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P26842 Related reagents
- Entrez Gene
- CD27 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0006915 apoptosis
- GO:0005576 extracellular region
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006916 anti-apoptosis
- GO:0006917 induction of apoptosis
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008588 release of cytoplasmic sequestered NF-kappaB
- GO:0016064 immunoglobulin mediated immune response
- GO:0043027 caspase inhibitor activity
- GO:0045579 positive regulation of B cell differentiation
- GO:0046330 positive regulation of JNK cascade
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD27 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/100 | ||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 | 1/100 | |
| Immunoprecipitation | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA929A647 | F | 100 Tests/1ml |
|
|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
|
| Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
|
|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
|
| Mouse IgG2a Negative Control:RPE | MCA929PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD27 antibody
-
Ravoet, A.M. et al. (1989) CD25 mAb: epitopes recognised, effect on lymphocyte activation, mediation of ADCC
in Leucocyte Typing IV: White Cell Differentiation Antigens Eds Knapp, W. et al. Oxford University Press pp 408-411. -
Boxall, S. et al. (2004) Abnormal cell surface antigen expression in individuals with variant CD45 splicing and histiocytosis.
Pediatr Res. 55 (3): 478-84. -
Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
Vet Immunol Immunopathol. 119: 115-22. -
Becker, P.D. et al. (2010) Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated ‘‘Human Immune System’’ Mice
PLoS One. 5(10). pii: e13137. -
van Zelm, M.C. et al. (2007) Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion.
J Exp Med. 204: 645-55. -
Steger, B. et al. (2014) CD4(+)and CD8(+)T-cell reactions against leukemia-associated- or minor-histocompatibility-antigens in AML-patients after allogeneic SCT.
Immunobiology. 219 (4): 247-60.
