CD222 antibody | MEM-238

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Mouse anti Human CD222:APC

Mouse anti Human CD222:FITC

Mouse anti Human CD222

Product Type
Monoclonal Antibody
Clone
MEM-238
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2048APC F* 100 Tests
MCA2048F F* 0.1 mg
MCA2048T F* 25 µg
MCA2048 F* IF 0.2 mg
Mouse anti Human CD222 antibody, clone MEM-238 recognizes human CD222, a 250 kDa transmembrane protein originally identified as the IGF II receptor. CD222 is ubiquitously expressed and is involved with internalization of a variety of ligands.

Mouse anti Human CD222 antibody, clone MEM-238 recognizes an epitope located in the region between extracellular domains 2 and 5 (aa 192-697) of CD222 (Roberts et al. 2010).

CD222 is primarily expressed intracellularly with a small percentage of molecules being located at the cell surface (5 - 10%).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant vaccinia virus containing CD222.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4ºC or at -20ºC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4ºC or at -20ºC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of reconstitution.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P11717 Related reagents
Entrez Gene
IGF2R Related reagents
GO Terms
GO:0001948 glycoprotein binding
GO:0005010 insulin-like growth factor receptor activity
GO:0005887 integral to plasma membrane
GO:0005215 transporter activity
GO:0005765 lysosomal membrane
GO:0005768 endosome
GO:0030139 endocytic vesicle
GO:0006898 receptor-mediated endocytosis
GO:0009986 cell surface
GO:0030140 trans-Golgi network transport vesicle
GO:0051219 phosphoprotein binding
Regulatory
For research purposes only

Applications of CD222 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 neat
Flow Cytometry 1 neat
Flow Cytometry 1 1/25 1/50
Immunofluorescence
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:APC MCA928APC 100 Tests F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD222 antibody

  1. Roberts, R.C. et al. (2010) Mistargeting of SH3TC2 away from the recycling endosome causes Charcot-Marie-Tooth disease type 4C.
    Hum Mol Genet. 19: 1009-18.
  2. Adachi, A. et al. (2010) Golgi-associated GSK3beta regulates the sorting process of post-Golgi membrane trafficking.
    J Cell Sci. 123: 3215-25.
  3. Godar, S. et al. (2002) CD222 (Mannose-6 phosphate / insulin-like growth factor II-receptor) Summary and Workshop Report.
    In Leucocyte Typing VII: White Cell Differentiation Antigens. Edited by Mason, D. et al. Oxford University Press. pp482-485.
  4. Rezgui, D. et al. (2009) Structure and function of the human Gly1619Arg polymorphism of M6P/IGF2R domain 11 implicated in IGF2 dependent growth.
    J Mol Endocrinol. 42: 341-56.
  5. McCormick, P.J. et al. (2008) Palmitoylation controls recycling in lysosomal sorting and trafficking.
    Traffic. 9: 1984-97.
  6. Leksa, V. et al. (2002) The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration.
    J Biol Chem. 277 (43): 40575-82.
  7. Osborne, D.G. et al. (2015) Monitoring receptor trafficking following retromer and WASH deregulation.
    Methods Cell Biol. 130: 199-213.