CD222 antibody | MEM-238
Mouse anti Human CD222 antibody, clone MEM-238 (MCA2048) use to detect CI-MPR (CD222) exprressionin vitro by immunofluorescence.
Image caption:
Rab7-mediated retrograde trafficking is enhanced in the absence of USP32. RPE1 NHT and USP32 KO cells were incubated with an antibody against the extracellular (luminal) domain of endogenous CI-MPR for 15 min at 37°C, followed by fixation and staining for the internalized antibody and TGN marker TGN46. Scale bar, 15 μm.
From: Hertel A, Alves LM, Dutz H, Tascher G, Bonn F, Kaulich M, Dikic I, Eimer S, Steinberg F, Bremm A.
USP32-regulated LAMTOR1 ubiquitination impacts mTORC1 activation and autophagy induction.
Cell Rep. 2022 Dec 6;41(10):111653.
doi: 10.1016/j.celrep.2022.111653. .
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD222 antibody, clone MEM-238 (MCA2048) use to detect CI-MPR (CD222) exprressionin vitro by western blotting.
Image caption:
Western blot analysis of whole cell lysates and lysosome fractions isolated from NHT and USP32 KO cells.
From: Hertel A, Alves LM, Dutz H, Tascher G, Bonn F, Kaulich M, Dikic I, Eimer S, Steinberg F, Bremm A.
USP32-regulated LAMTOR1 ubiquitination impacts mTORC1 activation and autophagy induction.
Cell Rep. 2022 Dec 6;41(10):111653.
doi: 10.1016/j.celrep.2022.111653. .
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F IF WB ResetMouse anti Human CD222
- Product Type
- Monoclonal Antibody
- Clone
- MEM-238
- Isotype
- IgG1
- Specificity
- CD222
Antibody Quality and Performance Guarantee
| Mouse anti Human CD222 antibody, clone MEM-238 recognizes human CD222, a 250 kDa transmembrane protein originally identified as the IGF II receptor. CD222 is ubiquitously expressed and is involved with internalization of a variety of ligands. Mouse anti Human CD222 antibody, clone MEM-238 recognizes an epitope located in the region between extracellular domains 2 and 5 (aa 192-697) of CD222 (Roberts et al. 2010). CD222 is primarily expressed intracellularly with a small percentage of molecules being located at the cell surface (5 - 10%). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- MCA2048T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA2048: Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Recombinant vaccinia virus containing CD222.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/25 | 1/50 |
| Immunofluorescence | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD222 antibody
-
Roberts, R.C. et al. (2010) Mistargeting of SH3TC2 away from the recycling endosome causes Charcot-Marie-Tooth disease type 4C.
Hum Mol Genet. 19: 1009-18. -
Adachi, A. et al. (2010) Golgi-associated GSK3beta regulates the sorting process of post-Golgi membrane trafficking.
J Cell Sci. 123: 3215-25. -
Godar, S. et al. (2002) CD222 (Mannose-6 phosphate / insulin-like growth factor II-receptor) Summary and Workshop Report.
In Leucocyte Typing VII: White Cell Differentiation Antigens. Edited by Mason, D. et al. Oxford University Press. pp482-485. -
Rezgui, D. et al. (2009) Structure and function of the human Gly1619Arg polymorphism of M6P/IGF2R domain 11 implicated in IGF2 dependent growth.
J Mol Endocrinol. 42: 341-56. -
McCormick, P.J. et al. (2008) Palmitoylation controls recycling in lysosomal sorting and trafficking.
Traffic. 9: 1984-97. -
Leksa, V. et al. (2002) The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration.
J Biol Chem. 277 (43): 40575-82. -
Osborne, D.G. et al. (2015) Monitoring receptor trafficking following retromer and WASH deregulation.
Methods Cell Biol. 130: 199-213. -
Hertel, A. et al. (2022) USP32-regulated LAMTOR1 ubiquitination impacts mTORC1 activation and autophagy induction.
Cell Rep. 41 (10): 111653.
View The Latest Product References
- Synonyms
- IGF-2 Receptor
- RRID
- AB_323432
- UniProt
- P11717
- Entrez Gene
- IGF2R
- GO Terms
- GO:0001948 glycoprotein binding
- GO:0005010 insulin-like growth factor receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005215 transporter activity
- GO:0005765 lysosomal membrane
- GO:0005768 endosome
- GO:0030139 endocytic vesicle
- GO:0006898 receptor-mediated endocytosis
- GO:0009986 cell surface
- View More GO Terms
- GO:0030140 trans-Golgi network transport vesicle
- GO:0051219 phosphoprotein binding
MCA2048T
1711
MCA2048
1801
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