CD222 antibody | MEM-238

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Anti Human CD222 Antibody, clone MEM-238 thumbnail image 1

Anti Human CD222 Antibody, clone MEM-238 thumbnail image 2

Anti Human CD222 Antibody, clone MEM-238 thumbnail image 3

Anti Human CD222 Antibody, clone MEM-238 gallery image 1 — Staining of permeabilized human peripheral blood granulocytes with Mouse anti Human CD222 (**MCA2048T**).

Anti Human CD222 Antibody, clone MEM-238 gallery image 2 — Staining of permeabilized human peripheral blood monocytes with Mouse anti Human CD222:FITC (**MCA2048F**).

Anti Human CD222 Antibody, clone MEM-238 gallery image 3 — Staining of permeabilized human peripheral blood granulocytes with Mouse anti Human CD222:APC (**MCA2048APC**).

Mouse anti Human CD222:APC

Mouse anti Human CD222:FITC

Mouse anti Human CD222

Product Type: Monoclonal Antibody
Clone: MEM-238
Isotype: IgG1

Product Code	Applications	Pack Size
MCA2048APC	F*	100 Tests
MCA2048F	F*	0.1 mg
MCA2048T	F*	25 µg
MCA2048	F* IF	0.2 mg

Mouse anti Human CD222 antibody, clone MEM-238 recognizes human CD222, a 250 kDa transmembrane protein originally identified as the IGF II receptor. CD222 is ubiquitously expressed and is involved with internalization of a variety of ligands.

Mouse anti Human CD222 antibody, clone MEM-238 recognizes an epitope located in the region between extracellular domains 2 and 5 (aa 192-697) of CD222 (Roberts et al. 2010).

CD222 is primarily expressed intracellularly with a small percentage of molecules being located at the cell surface (5 - 10%).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rhesus Monkey

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Allophycocyanin (APC) - lyophilised

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Reconstitution

Reconstitute with 1ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Preparation

Purified IgG prepared by affinity chromatography on Protein A from ascites

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Recombinant vaccinia virus containing CD222.

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4ºC or at -20ºC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4ºC or at -20ºC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of reconstitution.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P11717 Related reagents
Entrez Gene: IGF2R Related reagents
GO Terms: GO:0001948 glycoprotein binding; GO:0005010 insulin-like growth factor receptor activity; GO:0005887 integral to plasma membrane; GO:0005215 transporter activity; GO:0005765 lysosomal membrane; GO:0005768 endosome; GO:0030139 endocytic vesicle; GO:0006898 receptor-mediated endocytosis; GO:0009986 cell surface; GO:0030140 trans-Golgi network transport vesicle; GO:0051219 phosphoprotein binding
Regulatory: For research purposes only

Applications of CD222 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry ¹		neat
Flow Cytometry ¹		neat
Flow Cytometry ¹	1/25	1/50
Immunofluorescence

¹Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm^™ (Product Code BUF09) for this purpose.

¹Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm^™ (Product Code BUF09) for this purpose.

¹Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm^™ (Product Code BUF09) for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:APC	MCA928APC	100 Tests	F
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD222 antibody

Roberts, R.C. et al. (2010) Mistargeting of SH3TC2 away from the recycling endosome causes Charcot-Marie-Tooth disease type 4C.
Hum Mol Genet. 19: 1009-18.
Adachi, A. et al. (2010) Golgi-associated GSK3beta regulates the sorting process of post-Golgi membrane trafficking.
J Cell Sci. 123: 3215-25.
Godar, S. et al. (2002) CD222 (Mannose-6 phosphate / insulin-like growth factor II-receptor) Summary and Workshop Report.
In Leucocyte Typing VII: White Cell Differentiation Antigens. Edited by Mason, D. et al. Oxford University Press. pp482-485.
Rezgui, D. et al. (2009) Structure and function of the human Gly1619Arg polymorphism of M6P/IGF2R domain 11 implicated in IGF2 dependent growth.
J Mol Endocrinol. 42: 341-56.
McCormick, P.J. et al. (2008) Palmitoylation controls recycling in lysosomal sorting and trafficking.
Traffic. 9: 1984-97.
Leksa, V. et al. (2002) The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration.
J Biol Chem. 277 (43): 40575-82.
Osborne, D.G. et al. (2015) Monitoring receptor trafficking following retromer and WASH deregulation.
Methods Cell Biol. 130: 199-213.

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