CD222 antibody | MEM-238



Mouse anti Human CD222 antibody, clone MEM-238 recognizes an epitope located in the region between extracellular domains 2 and 5 (aa 192-697) of CD222 (Roberts et al. 2010).
CD222 is primarily expressed intracellularly with a small percentage of molecules being located at the cell surface (5 - 10%).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Recombinant vaccinia virus containing CD222.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4ºC or at -20ºC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4ºC or at -20ºC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P11717
- Entrez Gene
- IGF2R
- GO Terms
- GO:0001948 glycoprotein binding
- GO:0005010 insulin-like growth factor receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005215 transporter activity
- GO:0005765 lysosomal membrane
- GO:0005768 endosome
- GO:0030139 endocytic vesicle
- GO:0006898 receptor-mediated endocytosis
- GO:0009986 cell surface
- GO:0030140 trans-Golgi network transport vesicle
- GO:0051219 phosphoprotein binding
- Regulatory
- For research purposes only
Applications of CD222 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry 1 | neat | ||
Flow Cytometry 1 | 1/25 | 1/50 | |
Immunofluorescence |
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD222 antibody
-
Roberts, R.C. et al. (2010) Mistargeting of SH3TC2 away from the recycling endosome causes Charcot-Marie-Tooth disease type 4C.
Hum Mol Genet. 19: 1009-18. -
Adachi, A. et al. (2010) Golgi-associated GSK3beta regulates the sorting process of post-Golgi membrane trafficking.
J Cell Sci. 123: 3215-25. -
Godar, S. et al. (2002) CD222 (Mannose-6 phosphate / insulin-like growth factor II-receptor) Summary and Workshop Report.
In Leucocyte Typing VII: White Cell Differentiation Antigens. Edited by Mason, D. et al. Oxford University Press. pp482-485. -
Rezgui, D. et al. (2009) Structure and function of the human Gly1619Arg polymorphism of M6P/IGF2R domain 11 implicated in IGF2 dependent growth.
J Mol Endocrinol. 42: 341-56. -
McCormick, P.J. et al. (2008) Palmitoylation controls recycling in lysosomal sorting and trafficking.
Traffic. 9: 1984-97. -
Leksa, V. et al. (2002) The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration.
J Biol Chem. 277 (43): 40575-82. -
Osborne, D.G. et al. (2015) Monitoring receptor trafficking following retromer and WASH deregulation.
Methods Cell Biol. 130: 199-213.
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