CD19 antibody | LE-CD19
IP-MS Validated
Mouse anti Human CD19 antibody, clone LE-CD19 (MCA2454) used for the detection of B cells in human omental tissue by immunofluorescence.
Image caption:
pSTAT3-positive B cells are readily detectable in the omental tissues of ovarian cancer patients.
(A) Immunofluorescent staining followed by confocal microscopy showing examples of representative specimens from a patient with low B-cell infiltration and low pSTAT3 expression (Top- left and right), and a separate patient with high B-cell infiltration and high pSTAT3 expression (bottom left and right); scale bars, 20 μm.
From: Yang C, Lee H, Jove V, Deng J, Zhang W, Liu X, et al.
(2013) Prognostic Significance of B-Cells and pSTAT3 in Patients with Ovarian Cancer.
PLoS ONE 8(1): e54029.
doi: 10.1371/journal.pone.0054029
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LE-CD19 (MCA2454) used to evaluate CD19 expression on human B cells by immunofluorescence.
Image caption:
Quantification of γH2AX foci in (a) CD19+ B cells (CD19, green; γH2AX, red) of patients following total body radiation. Representative images are shown. (a) B cells: 20 CD19+ B cells were quantified per sample. No CD19+ B cells could be found on the slide at d3 (n.d., not detectable). In general, there were only small amounts of γH2AX foci induced in B cells, with a maximum of 1 focus per cell at day 2 (t-test, *p <0,05).
From: Heylmann D, Ponath V, Kindler T, Kaina B.
Comparison of DNA repair and radiosensitivity of different blood cell populations.
Sci Rep. 2021 Jan 28;11(1):2478.
doi: 10.1038/s41598-021-81058-1..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LE-CD19 (MCA2454) used to evaluate CD19 expression on human B cells by immunofluorescence.
Image caption:
Staining of γH2AX (green) and CD surface markers (red) of different blood cell types purified from buffy coat. (a) Representative pictures of unirradiated cells (control) and samples 1 and 24 h after 2 Gy IR. A maximum of γH2AX foci accumulate 1 h after exposure to 2 Gy. γH2AX foci disappear 24 h after radiation implicating effective repair of IR-induced DNA double-strand breaks. CD19+ B cells are hardly detectable (h.d.) on the slide 24 h after treatment. Furthermore, no CD34+ progenitor cells could be found on the slide 24 h post-irradiation (n.d. not detectable). Granulocytes, shown in combination with the CD15 marker, did not show any IR-induced formation of γ H2AX foci.
From: Heylmann D, Ponath V, Kindler T, Kaina B.
Comparison of DNA repair and radiosensitivity of different blood cell populations.
Sci Rep. 2021 Jan 28;11(1):2478.
doi: 10.1038/s41598-021-81058-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LE-CD19 (MCA2454) used for the detection of B cells in human omental tissue by immunohistochemistry.
Image caption:
pSTAT3-positive B cells are readily detectable in the omental tissues of ovarian cancer patients.
(B) IHC images showing B cells and pSTAT3-positive cells in the same area of omental tissues; scale bars, 200 μm.
From: Yang C, Lee H, Jove V, Deng J, Zhang W, Liu X, et al.
(2013) Prognostic Significance of B-Cells and pSTAT3 in Patients with Ovarian Cancer.
PLoS ONE 8(1): e54029.
doi: 10.1371/journal.pone.0054029
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LE-CD19 (MCA2454) staining of human tumor masses by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative histological markers detected on tumors.
High magnification (20X) of histological sections of tumors developing after in vivo injection of VR09 cell line. Lymphoid infiltrates display large size, plasmablastic-plasmacytic features and high Ki-67 index. Cells express CD19, CD20, CD138, CD79a, IgM, IgG and EBV protein (EBER).
From: Nichele I, Zamò A, Bertolaso A, Bifari F, Tinelli M, Franchini M, et al. (2012)
VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type.
PLoS ONE 7(12): e52811.
doi: 10.1371/journal.pone.0052811
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LE-CD19 recognizes an epitope within the C-terminal cytoplasmic tail sequence of human CD19, a single pass type I transmembrane glycoprotein containing two C2 type Ig-like domains in the N-terminal extracellular region and four potential phosphorylation sites for tyrosine together with a single serine in the cytoplasmic region.
Human CD19 is expressed on virtually all cells of the B-cell lineage with the exception of plasma cells and plays a regulatory role in B-cell differentiation and proliferation.
B-cells are essential for antibody production and mutations in the CD19 gene can lead to an immunodeficiency syndrome, CIVD3 characterized by hypogammaglobulinemia leading to recurrent infections and the inability to mount an antibody mediated response to immune insult. Although immunoglobulin production is impaired B-cell precursors appear in normal numbers together with some reduction in more mature B-cell forms (van Zelm et al. 2006). B-cells have also been implicated in the progression and pathogenesis of multiple sclerosis and are common components of both active and chronic MS lesions and well as the CSF (Ritchie et al. 2004)
Mouse anti Human CD19 antibody, clone LE-CD19 has been successfully employed for the immunohistochemical demonstration of CD19 in formalin fixed, paraffin embedded tissues (Streeck, H. et al. 2011) and for the detection of CD19 in cell lysates by Western blotting.
Human CD19 is expressed on virtually all cells of the B-cell lineage with the exception of plasma cells and plays a regulatory role in B-cell differentiation and proliferation.
B-cells are essential for antibody production and mutations in the CD19 gene can lead to an immunodeficiency syndrome, CIVD3 characterized by hypogammaglobulinemia leading to recurrent infections and the inability to mount an antibody mediated response to immune insult. Although immunoglobulin production is impaired B-cell precursors appear in normal numbers together with some reduction in more mature B-cell forms (van Zelm et al. 2006). B-cells have also been implicated in the progression and pathogenesis of multiple sclerosis and are common components of both active and chronic MS lesions and well as the CSF (Ritchie et al. 2004)
Mouse anti Human CD19 antibody, clone LE-CD19 has been successfully employed for the immunohistochemical demonstration of CD19 in formalin fixed, paraffin embedded tissues (Streeck, H. et al. 2011) and for the detection of CD19 in cell lysates by Western blotting.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- CD19 peptide CGPDPAWGGGGRMGTWSTR (C-terminus) coupled to KLH.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P15391
- Entrez Gene
- CD19
- GO Terms
- GO:0005515 protein binding
- GO:0005057 receptor signaling protein activity
- GO:0005887 integral to plasma membrane
- GO:0006968 cellular defense response
- Regulatory
- For research purposes only
Applications of CD19 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry 1 | 1/100 | 1/200 | |
| Immunohistology - Paraffin 2 | 1/100 | 1/200 | |
| Immunoprecipitation | |||
| Western Blotting | Reducing conditions |
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Tonsil
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for CD19 antibody
-
Ogembo, J.G. et al. (2012) SIRPα/CD172a and FHOD1 Are Unique Markers of Littoral Cells, a Recently Evolved Major Cell Population of Red Pulp of Human Spleen.
J Immunol. 188: 4496-505. -
Streeck, H. et al. (2011) Epithelial adhesion molecules can inhibit HIV-1-specific CD8⁺ T-cell functions
Blood. 117: 5112-22. -
Herting, F. et al. (2014) Enhanced anti-tumor activity of the glycoengineered type II CD20 antibody obinutuzumab (GA101) in combination with chemotherapy in xenograft models of human lymphoma.
Leuk Lymphoma. 55 (9): 2151-60. -
Inoue, T. et al. (2010) Differential expression of glycogenes in tonsillar B lymphocytes in association with proteinuria and renal dysfunction in IgA nephropathy.
Clin Immunol. 136 (3): 447-55. -
Yang, C. et al. (2013) Prognostic significance of B-cells and pSTAT3 in patients with ovarian cancer.
PLoS One. 8 (1): e54029. -
Nichele, I. et al. (2012) VR09 cell line: an EBV-positive lymphoblastoid cell line with in vivo. characteristics of diffuse large B cell lymphoma of activated B-cell type.
PLoS One. 7 (12): e52811. -
Heylmann, D. et al. (2021) Comparison of DNA repair and radiosensitivity of different blood cell populations.
Sci Rep. 11 (1): 2478.
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