CD19 antibody | Bu12

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Anti Human CD19 Antibody, clone Bu12 thumbnail image 1

Anti Human CD19 Antibody, clone Bu12 thumbnail image 2

Anti Human CD19 Antibody, clone Bu12 thumbnail image 3

Anti Human CD19 Antibody, clone Bu12 gallery image 1 — Figure A. A647 conjugated Mouse anti Human CD20 (MCA1710A647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. A647 conjugated Mouse anti Human CD20 (MCA1710A647) and FITC conjugated Mouse anti Human CD19 (**MCA2495F**). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Anti Human CD19 Antibody, clone Bu12 gallery image 2 — Figure A. Alexa Fluor488 conjugated mouse anti human CD20 (MCA1710A488) and Alexa Fluor647 conjugated mouse IgG1 isotype control (MCA928A647). Figure B. Alexa Fluor488 conjugated mouse anti human CD20 (MCA1710A488) and Alexa Fluor647 conjugated mouse anti human CD19 (**MCA2495A647**). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5™ cell analyzer.

Anti Human CD19 Antibody, clone Bu12 gallery image 3 — Figure A. Alexa Fluor700 conjugated mouse anti human CD20 (MCA1710A700) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. Alexa Fluor700 conjugated mouse anti human CD20 (MCA1710A700) and RPE conjugated mouse anti human CD19 (**MCA2495PE**). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5™ cell analyzer.

Mouse anti Human CD19:Alexa Fluor® 647

Mouse anti Human CD19:FITC

Mouse anti Human CD19

Mouse anti Human CD19:RPE

Product Type: Monoclonal Antibody
Clone: Bu12
Isotype: IgG1

Product Code	Applications	Pack Size
MCA2495A647	F	100 Tests/1ml
MCA2495F	F	0.1 mg
MCA2495T	F IP	25 µg
MCA2495	F IP	0.2 mg
MCA2495PE	F	100 Tests

Mouse anti Human CD19 antibody, clone Bu12 recognizes human CD19, a 95 kDa cell surface glycoprotein, which is expressed on cells of the B cell lineage and follicular dendritic cells but absent on plasma cells. CD19 is an important signal transduction molecule which is involved in the regulation of B lymphocyte development, activation and differentiation.

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Alexa Fluor 647 - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1.0 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide (NaN₃)
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Carrier Free

Yes

Immunogen

Human EB-4 Burkitt lymphoma cell line.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Fusion Partners

Spleen cells from immunized mice were fused with cells from the X63 AG8 653 plasmacytoma.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee: 18 months from date of despatch
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution

More Information

UniProt: P15391 Related reagents
Entrez Gene: CD19 Related reagents
GO Terms: GO:0005515 protein binding; GO:0005057 receptor signaling protein activity; GO:0005887 integral to plasma membrane; GO:0006968 cellular defense response
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD19 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat
Flow Cytometry	Neat	1/10
Flow Cytometry	1/50	1/200
Immunoprecipitation
Flow Cytometry	Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	100 Tests/1ml	F
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Product Specific References

References for CD19 antibody

Zhou, L. and Tedder, T. (1993) CD19 Workshop panel report.
In leucocyte Typing V. White cell differentiation antigens. Oxford University press. p507 – 509.
Smith SH et al. (1991) Activation of human B cells through the CD19 surface antigen results in homotypic adhesion by LFA-1-dependent and -independent mechanisms.
Immunology. 73 (3): 293-7.
Callard, R.E. et al. (1992) CD19 regulation of human B cell responses. B cell proliferation and antibody secretion are inhibited or enhanced by ligation of the CD19 surface glycoprotein depending on the stimulating signal used.
J Immunol. 148 (10): 2983-7.
Callard, R.E. et al. (1995) CD40 cross-linking inhibits specific antibody production by human B cells.
Int Immunol. 7 (11): 1809-15.
Flavell, D.J. et al. (1995) Preclinical studies with the anti-CD19-saporin immunotoxin BU12-SAPORIN for the treatment of human-B-cell tumours.
Br J Cancer. 72 (6): 1373-9.
Vallera, D. A. et al. (2004) Radiotherapy of CD19 expressing Daudi tumors in nude mice with Yttrium-90-labeled anti-CD19 antibody.
Cancer Biother Radiopharm. 19 (1): 11-23.

CD19 resources

CD19 - The marker, the antibodies

CD19 articles & blog posts

Posters, guides and online database

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