CD178 antibody | 14C2
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|Mouse anti Human CD178 antibody, clone 14C2 recognizes the human CD178, also known as Tumor necrosis factor ligand superfamily member 6, Fas ligand (FasL), Apoptosis antigen ligand or CD95 ligand. CD178 is a 281 amino acid, a ~40 kDa single pass type-II transmembrane glycoprotein bearing a single intracellular FasL domain and member of the tumor necrosis factor family .
CD178 is expressed by activated T lymphocytes and NK cells (Leite-de-Moraes and Dy 1997). The protein may exist as either a membrane bound or a cleaved soluble form (Garcia et al. 2013). CD178 plays an important role in T cell mediated cytotoxicity (Jodo et al. 2005). Binding of CD178 to Fas (CD95) results in the induction of apoptosis (Ju et al. 1995).
Mouse anti human CD178 antibody, clone 14C2 is reported to recognize a conformation dependent non-blocking epitope on CD178 (Daburon et al. 2013).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1||1/25||1/50|
- 1 Results maybe enhanced using membrane permeabilisation. Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD178 antibody
Legembre, P. et al. (2005) Amplification of Fas-mediated apoptosis in type II cells via microdomain recruitment.
Mol Cell Biol. 25 (15): 6811-20.
Mesdaghi, M. et al. (2010) Natural killer cells in allergic rhinitis patients and nonatopic controls.
Int Arch Allergy Immunol. 153 (3): 234-8.
Li, R. et al. (2014) Human heat shock protein-specific cytotoxic T lymphocytes display potent antitumour immunity in multiple myeloma.
Br J Haematol. 166 (5): 690-701.
Ouwendijk, W.J. et al. (2014) Functional characterization of ocular-derived human alphaherpesvirus cross-reactive CD4 T cells.
J Immunol. 192: 3730-9.
Matzner, P. et al. (2013) Resilience of the immune system in healthy young students to 30-hour sleep deprivation with psychological stress.
Neuroimmunomodulation. 20: 194-204.
Sullivan, E.M. et al. (2014) NK cell genotype and phenotype at diagnosis of acute lymphoblastic leukemia correlate with postinduction residual disease.
Clin Cancer Res. 20 (23): 5986-94.
Lindqvist CA et al. (2011) Both CD4+ FoxP3+ and CD4+ FoxP3- T cells from patients with B-cell malignancy express cytolytic markers and kill autologous leukaemic B cells in vitro.
Immunology. 133 (3): 296-306.
Holmannova D et al. (2015) Inhibitory CD200R and proapoptotic CD95/CD95L molecules on innate immunity cells are modulated by cardiac surgery.
Perfusion. 30 (7): 543-55.
View The Latest Product References
Tomchuck, S.L. et al. (2015) Enhanced cytotoxic function of natural killer and CD3+CD56+ cells in cord blood after culture.
Biol Blood Marrow Transplant. 21 (1): 39-49.
Pachnio, A. et al. (2016) Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium.
PLoS Pathog. 12 (9): e1005832.
Fathalla, A.M. et al. (2020) Polymyxin-Induced Cell Death of Human Macrophage-Like THP-1 and Neutrophil-Like HL-60 Cells Associated with the Activation of Apoptotic Pathways.
Antimicrob Agents Chemother. 64 (9)Aug 20 [Epub ahead of print].
- FAS Ligand
- Entrez Gene
- GO Terms
- GO:0007165 signal transduction
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006919 activation of caspase activity
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0008633 activation of pro-apoptotic gene products
- GO:0016525 negative regulation of angiogenesis
- View More GO Terms
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0060554 induction of necroptosis of activated-T cells
- GO:0060555 induction of necroptosis by extracellular signals
- GO:0070265 necrotic cell death
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