CD178 antibody | 14C2

Mouse anti Human CD178

Product Type
Monoclonal Antibody
Clone
14C2
Isotype
IgG1
Specificity
CD178

Product Code Applications Pack Size List Price Your Price Qty
MCA2409GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * IP 0.1 mg loader
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loader
MCA2409
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * IP 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD178 antibody, clone 14C2 recognizes the human CD178, also known as Tumor necrosis factor ligand superfamily member 6, Fas ligand (FasL), Apoptosis antigen ligand or CD95 ligand. CD178 is a 281 amino acid, a ~40 kDa single pass type-II transmembrane glycoprotein bearing a single intracellular FasL domain and member of the tumor necrosis factor family .

CD178 is expressed by activated T lymphocytes and NK cells (Leite-de-Moraes and Dy 1997). The protein may exist as either a membrane bound or a cleaved soluble form (Garcia et al. 2013). CD178 plays an important role in T cell mediated cytotoxicity (Jodo et al. 2005). Binding of CD178 to Fas (CD95) results in the induction of apoptosis (Ju et al. 1995).

Mouse anti human CD178 antibody, clone 14C2 is reported to recognize a conformation dependent non-blocking epitope on CD178 (Daburon et al. 2013).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/25 1/50
Immunoprecipitation
Western Blotting
  1. 1 Results maybe enhanced using membrane permeabilisation. Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Mouse IgG1 Negative Control

References for CD178 antibody

  1. Legembre, P. et al. (2005) Amplification of Fas-mediated apoptosis in type II cells via microdomain recruitment.
    Mol Cell Biol. 25 (15): 6811-20.
  2. Mesdaghi, M. et al. (2010) Natural killer cells in allergic rhinitis patients and nonatopic controls.
    Int Arch Allergy Immunol. 153 (3): 234-8.
  3. Li, R. et al. (2014) Human heat shock protein-specific cytotoxic T lymphocytes display potent antitumour immunity in multiple myeloma.
    Br J Haematol. 166 (5): 690-701.
  4. Ouwendijk, W.J. et al. (2014) Functional characterization of ocular-derived human alphaherpesvirus cross-reactive CD4 T cells.
    J Immunol. 192: 3730-9.
  5. Matzner, P. et al. (2013) Resilience of the immune system in healthy young students to 30-hour sleep deprivation with psychological stress.
    Neuroimmunomodulation. 20: 194-204.
  6. Sullivan, E.M. et al. (2014) NK cell genotype and phenotype at diagnosis of acute lymphoblastic leukemia correlate with postinduction residual disease.
    Clin Cancer Res. 20 (23): 5986-94.
  7. Lindqvist CA et al. (2011) Both CD4+ FoxP3+ and CD4+ FoxP3- T cells from patients with B-cell malignancy express cytolytic markers and kill autologous leukaemic B cells in vitro.
    Immunology. 133 (3): 296-306.
  8. Holmannova D et al. (2015) Inhibitory CD200R and proapoptotic CD95/CD95L molecules on innate immunity cells are modulated by cardiac surgery.
    Perfusion. 30 (7): 543-55.
  9. View The Latest Product References
  10. Tomchuck, S.L. et al. (2015) Enhanced cytotoxic function of natural killer and CD3+CD56+ cells in cord blood after culture.
    Biol Blood Marrow Transplant. 21 (1): 39-49.
  11. Pachnio, A. et al. (2016) Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium.
    PLoS Pathog. 12 (9): e1005832.
  12. Fathalla, A.M. et al. (2020) Polymyxin-Induced Cell Death of Human Macrophage-Like THP-1 and Neutrophil-Like HL-60 Cells Associated with the Activation of Apoptotic Pathways.
    Antimicrob Agents Chemother. 64 (9)Aug 20 [Epub ahead of print].

Flow Cytometry

Synonyms
FAS Ligand
RRID
AB_566573
UniProt
P48023
Entrez Gene
FASLG
GO Terms
GO:0007165 signal transduction
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006919 activation of caspase activity
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008633 activation of pro-apoptotic gene products
GO:0016525 negative regulation of angiogenesis
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0060554 induction of necroptosis of activated-T cells
GO:0060555 induction of necroptosis by extracellular signals
GO:0070265 necrotic cell death
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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