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CD178 antibody | 14C2

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Anti Human CD178 Antibody, clone 14C2 thumbnail image 1 Anti Human CD178 Antibody, clone 14C2 thumbnail image 2 Anti Human CD178 Antibody, clone 14C2 thumbnail image 3 Anti Human CD178 Antibody, clone 14C2 thumbnail image 4 Anti Human CD178 Antibody, clone 14C2 thumbnail image 5 Anti Human CD178 Antibody, clone 14C2 thumbnail image 6
Anti Human CD178 Antibody, clone 14C2 gallery image 1
Staining of CD178 transfected CHO cells with Alexa Fluor ® 647 conjugated Mouse anti Human CD178 antibody (MCA2409A647)
Anti Human CD178 Antibody, clone 14C2 gallery image 2
Staining of permeabilised Jurkat cells with Mouse anti Human CD178 (MCA2409)
Anti Human CD178 Antibody, clone 14C2 gallery image 3
Staining of permeabilized Jurkat cells with Mouse anti Human CD178 (MCA2409GA)
Anti Human CD178 Antibody, clone 14C2 gallery image 4
Staining of permeabilized Jurkat cells with Alexa Fluor® 488 conjugated Mouse anti Human CD178 antibody (MCA2409A488)
Anti Human CD178 Antibody, clone 14C2 gallery image 5
Staining of permeabilized Jurkat cells with phycoerythrin conjugated Mouse anti Human CD178 antibody (MCA2409PE)
Anti Human CD178 Antibody, clone 14C2 gallery image 6
Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 (MCA2409) as a capture reagent and biotinylated Mouse anti Human CD178 as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma (orange) sample is displayed at 1:2 dilution.

Mouse anti Human CD178:Alexa Fluor® 488

Mouse anti Human CD178:Alexa Fluor® 647

Mouse anti Human CD178

Mouse anti Human CD178:RPE

Product Type
Monoclonal Antibody
Clone
14C2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2409A488T
Datasheet
F*
SDS
25 Tests/0.25ml loader

MCA2409A488
Datasheet
F*
SDS
100 Tests/1ml loader

MCA2409A647
Datasheet
F*
SDS
100 Tests/1ml loader

MCA2409A647T
Datasheet
F*
SDS
25 Tests/0.25ml loader

MCA2409GA
Datasheet
E F* IP
SDS
0.1 mg loader

MCA2409
Datasheet
E F* IP
SDS
0.2 mg loader

MCA2409PE
Datasheet
F*
SDS
100 Tests loader

Mouse anti Human CD178 antibody, clone 14C2 recognizes the human CD178, also known as Tumor necrosis factor ligand superfamily member 6, Fas ligand (FasL), Apoptosis antigen ligand or CD95 ligand. CD178 is a 281 amino acid, a ~40 kDa single pass type-II transmembrane glycoprotein bearing a single intracellular FasL domain and member of the tumor necrosis factor family .

CD178 is expressed by activated T lymphocytes and NK cells (Leite-de-Moraes and Dy 1997). The protein may exist as either a membrane bound or a cleaved soluble form (Garcia et al. 2013). CD178 plays an important role in T cell mediated cytotoxicity (Jodo et al. 2005). Binding of CD178 to Fas (CD95) results in the induction of apoptosis (Ju et al. 1995).

Mouse anti human CD178 antibody, clone 14C2 is reported to recognize a conformation dependent non-blocking epitope on CD178 (Daburon et al. 2013).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P48023
Entrez Gene
FASLG
GO Terms
GO:0007165 signal transduction
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006919 activation of caspase activity
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008633 activation of pro-apoptotic gene products
GO:0016525 negative regulation of angiogenesis
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0060554 induction of necroptosis of activated-T cells
GO:0060555 induction of necroptosis by extracellular signals
GO:0070265 necrotic cell death
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD178 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
Flow Cytometry 1 Neat
ELISA
Flow Cytometry 1 1/25 1/50
Immunoprecipitation
Western Blotting
Flow Cytometry 1 Neat
  1. 1 Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of Leucoperm™ (Product code BUF09) prior to staining.
  1. 1 Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
  1. 1 Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
  1. 1 Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Product Specific References

References for CD178 antibody

  1. Legembre, P. et al. (2005) Amplification of Fas-mediated apoptosis in type II cells via microdomain recruitment.
    Mol Cell Biol. 25 (15): 6811-20.
  2. Mesdaghi, M. et al. (2010) Natural killer cells in allergic rhinitis patients and nonatopic controls.
    Int Arch Allergy Immunol. 153 (3): 234-8.
  3. Li, R. et al. (2014) Human heat shock protein-specific cytotoxic T lymphocytes display potent antitumour immunity in multiple myeloma.
    Br J Haematol. 166 (5): 690-701.
  4. Ouwendijk, W.J. et al. (2014) Functional characterization of ocular-derived human alphaherpesvirus cross-reactive CD4 T cells.
    J Immunol. 192: 3730-9.
  5. Matzner, P. et al. (2013) Resilience of the immune system in healthy young students to 30-hour sleep deprivation with psychological stress.
    Neuroimmunomodulation. 20: 194-204.
  6. Sullivan, E.M. et al. (2014) NK cell genotype and phenotype at diagnosis of acute lymphoblastic leukemia correlate with postinduction residual disease.
    Clin Cancer Res. 20 (23): 5986-94.
  7. Lindqvist CA et al. (2011) Both CD4+ FoxP3+ and CD4+ FoxP3- T cells from patients with B-cell malignancy express cytolytic markers and kill autologous leukaemic B cells in vitro.
    Immunology. 133 (3): 296-306.
  8. Holmannova D et al. (2015) Inhibitory CD200R and proapoptotic CD95/CD95L molecules on innate immunity cells are modulated by cardiac surgery.
    Perfusion. 30 (7): 543-55.
  9. Pachnio, A. et al. (2016) Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium.
    PLoS Pathog. 12 (9): e1005832.
  10. Fathalla, A.M. et al. (2020) Polymyxin-Induced Cell Death of Human Macrophage-Like THP-1 and Neutrophil-Like HL-60 Cells Associated with the Activation of Apoptotic Pathways.
    Antimicrob Agents Chemother. 64 (9)Aug 20 [Epub ahead of print].

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