CD178 antibody | 14C2
Mouse anti Human CD178:Alexa Fluor® 488
Mouse anti Human CD178:Alexa Fluor® 647
Mouse anti Human CD178
Mouse anti Human CD178:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 14C2
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA2409A488 | F* | 100 Tests/1ml |
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| MCA2409A488T | F* | 25 Tests/0.25ml |
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| MCA2409A647 | F* | 100 Tests/1ml |
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| MCA2409A647T | F* | 25 Tests/0.25ml |
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| MCA2409GA | E F* IP | 0.1 mg |
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| MCA2409 | E F* IP | 0.2 mg |
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| MCA2409PE | F* | 100 Tests |
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Mouse anti Human CD178 antibody, clone 14C2 recognizes the human CD178, also known as Tumor necrosis factor ligand superfamily member 6, Fas ligand (FasL), Apoptosis antigen ligand or CD95 ligand. CD178 is a 281 amino acid, a ~40 kDa single pass type-II transmembrane glycoprotein bearing a single intracellular FasL domain and member of the tumor necrosis factor family .
CD178 is expressed by activated T lymphocytes and NK cells (Leite-de-Moraes and Dy 1997). The protein may exist as either a membrane bound or a cleaved soluble form (Garcia et al. 2013). CD178 plays an important role in T cell mediated cytotoxicity (Jodo et al. 2005). Binding of CD178 to Fas (CD95) results in the induction of apoptosis (Ju et al. 1995).
Mouse anti human CD178 antibody, clone 14C2 is reported to recognize a conformation dependent non-blocking epitope on CD178 (Daburon et al. 2013).
CD178 is expressed by activated T lymphocytes and NK cells (Leite-de-Moraes and Dy 1997). The protein may exist as either a membrane bound or a cleaved soluble form (Garcia et al. 2013). CD178 plays an important role in T cell mediated cytotoxicity (Jodo et al. 2005). Binding of CD178 to Fas (CD95) results in the induction of apoptosis (Ju et al. 1995).
Mouse anti human CD178 antibody, clone 14C2 is reported to recognize a conformation dependent non-blocking epitope on CD178 (Daburon et al. 2013).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P48023
- Entrez Gene
- FASLG
- GO Terms
- GO:0007165 signal transduction
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006919 activation of caspase activity
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0008633 activation of pro-apoptotic gene products
- GO:0016525 negative regulation of angiogenesis
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0060554 induction of necroptosis of activated-T cells
- GO:0060555 induction of necroptosis by extracellular signals
- GO:0070265 necrotic cell death
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD178 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | Neat | ||
| Flow Cytometry 1 | Neat | ||
| ELISA | |||
| Flow Cytometry 1 | 1/25 | 1/50 | |
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry 1 | Neat |
- 1 Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
- 1 Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
- 1 Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
- 1 Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
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| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
|
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| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD178 antibody
-
Legembre, P. et al. (2005) Amplification of Fas-mediated apoptosis in type II cells via microdomain recruitment.
Mol Cell Biol. 25 (15): 6811-20. -
Mesdaghi, M. et al. (2010) Natural killer cells in allergic rhinitis patients and nonatopic controls.
Int Arch Allergy Immunol. 153 (3): 234-8. -
Li, R. et al. (2014) Human heat shock protein-specific cytotoxic T lymphocytes display potent antitumour immunity in multiple myeloma.
Br J Haematol. 166 (5): 690-701. -
Ouwendijk, W.J. et al. (2014) Functional characterization of ocular-derived human alphaherpesvirus cross-reactive CD4 T cells.
J Immunol. 192: 3730-9. -
Matzner, P. et al. (2013) Resilience of the immune system in healthy young students to 30-hour sleep deprivation with psychological stress.
Neuroimmunomodulation. 20: 194-204. -
Sullivan, E.M. et al. (2014) NK cell genotype and phenotype at diagnosis of acute lymphoblastic leukemia correlate with postinduction residual disease.
Clin Cancer Res. 20 (23): 5986-94. -
Lindqvist CA et al. (2011) Both CD4+ FoxP3+ and CD4+ FoxP3- T cells from patients with B-cell malignancy express cytolytic markers and kill autologous leukaemic B cells in vitro.
Immunology. 133 (3): 296-306. -
Holmannova D et al. (2015) Inhibitory CD200R and proapoptotic CD95/CD95L molecules on innate immunity cells are modulated by cardiac surgery.
Perfusion. 30 (7): 543-55. -
Pachnio, A. et al. (2016) Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium.
PLoS Pathog. 12 (9): e1005832. -
Fathalla, A.M. et al. (2020) Polymyxin-Induced Cell Death of Human Macrophage-Like THP-1 and Neutrophil-Like HL-60 Cells Associated with the Activation of Apoptotic Pathways.
Antimicrob Agents Chemother. 64 (9)Aug 20 [Epub ahead of print].
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