CD178 antibody | 10F2

Mouse anti Human CD178

Product Type
Monoclonal Antibody
Clone
10F2
Isotype
IgG1
Specificity
CD178

Product Code Applications Pack Size List Price Your Price Qty
MCA2408GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IP 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD178 antibody, clone 10F2 recognizes the human CD178 cell surface antigen, a ~40 kDa glycoprotein also known as Fas ligand (CD95L).

CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form.

CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.

Mouse anti Human CD178 antibody, clone 10F2 is reported to block the activity of Fas ligand (Legembre et al. 2002).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/25 1/50
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD178 antibody

  1. Legembre, P. et al. (2002) Potentiation of Fas-mediated apoptosis by an engineered glycosylphosphatidylinositol-linked Fas.
    Cell Death Differ. 9 (3): 329-39.
  2. Ferry-Dumazet, H. et al. (2002) Resveratrol inhibits the growth and induces the apoptosis of both normal and leukemic hematopoietic cells.
    Carcinogenesis. 23 (8): 1327-33.
  3. Thorén, F.B. et al. (2012) Human NK Cells induce neutrophil apoptosis via an NKp46- and Fas-dependent mechanism.
    J Immunol. 188 (4): 1668-74.
  4. Robinet, P. et al. (2014) A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
    J Immunol. 192 (11): 5332-42.
  5. Pellegrin, S. et al. (2012) Differential proteomic analysis of human erythroblasts undergoing apoptosis induced by epo-withdrawal.
    PLoS One. 7 (6): e38356.
  6. Morello, A. et al. (2013) Enhancing production and cytotoxic activity of polymeric soluble FasL-based chimeric proteins by concomitant expression of soluble FasL.
    PLoS One. 8 (8): e73375.
  7. Pugholm, L, H. et al. (2016) Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles
    J Immunol Res. 2016: 1-12.

Synonyms
FAS Ligand
RRID
AB_566571
UniProt
P48023
Entrez Gene
FASLG
GO Terms
GO:0007165 signal transduction
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006919 activation of caspase activity
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008633 activation of pro-apoptotic gene products
GO:0016525 negative regulation of angiogenesis
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0060554 induction of necroptosis of activated-T cells
GO:0060555 induction of necroptosis by extracellular signals
GO:0070265 necrotic cell death
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products