CD178 Antibody | 14C2

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CD178 Antibody | 14C2 gallery image 1

Staining of CD178 transfected CHO cells with Mouse anti Human CD178: Alexa Fluor® 647 (MCA2409A647)

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CD178 Antibody | 14C2 gallery image 2

Staining of permeabilised Jurkat cells with Mouse anti Human CD178 (MCA2409)

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CD178 Antibody | 14C2 gallery image 3

Staining of permeabilised Jurkat cells with Mouse anti Human CD178 (MCA2409GA)

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CD178 Antibody | 14C2 gallery image 4

Staining of permeabilised Jurkat cells with Mouse anti Human CD178:Alexa Fluor® 488 (MCA2409A488)

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CD178 Antibody | 14C2 gallery image 5

Staining of permeabilised Jurkat cells with Mouse anti Human CD178:RPE (MCA2409PE)

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CD178 Antibody | 14C2 gallery image 6

Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 (MCA2409) as a capture reagent and biotinylated Mouse anti Human CD178 (MCA2408) as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma (orange) sample is displayed at 1:2 dilution.

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  • Mouse anti Human CD178
  • Mouse anti Human CD178:Alexa Fluor® 488
  • Mouse anti Human CD178
  • Mouse anti Human CD178:Alexa Fluor® 647
  • Mouse anti Human CD178:RPE
  • Mouse anti Human CD178
  • Mouse anti Human CD178:RPE
  • Mouse anti Human CD178:Alexa Fluor® 488
  • Mouse anti Human CD178:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    14C2
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2409GAE, F *, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2409GA
    MCA2409E, F *, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA2409
    MCA2409PEF *datasheet pdfdatasheet pdf100 Tests
    MCA2409PE
    MCA2409A647F *datasheet pdfdatasheet pdf100 Tests/1ml
    MCA2409A647
    MCA2409A488F *datasheet pdfdatasheet pdf100 Tests/1ml
    MCA2409A488
    MCA2409TE, F *, IPdatasheet pdfdatasheet pdf25 µg
    MCA2409T
    MCA2409A488TF *datasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2409A488T
    MCA2409PETF *datasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2409PET
    MCA2409A647TF *datasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2409A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD178 antibody, clone 14C2 recognizes the human CD178, also known as Tumor necrosis factor ligand superfamily member 6, Fas ligand (FasL), Apoptosis antigen ligand or CD95 ligand. CD178 is a 281 amino acid, a ~40 kDa single pass type-II transmembrane glycoprotein bearing a single intracellular FasL domain and member of the tumor necrosis factor family .

      CD178 is expressed by activated T lymphocytes and NK cells (Leite-de-Moraes and Dy 1997). The protein may exist as either a membrane bound or a cleaved soluble form (Garcia et al. 2013). CD178 plays an important role in T cell mediated cytotoxicity (Jodo et al. 2005). Binding of CD178 to Fas (CD95) results in the induction of apoptosis (Ju et al. 1995).

      Mouse anti human CD178 antibody, clone 14C2 is reported to recognize a conformation dependent non-blocking epitope on CD178 (Daburon et al. 2013).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 Tests/0.25mlStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 Tests/0.25mlStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      induction of necroptosis by extracellular signals
      cytokine activity
      signal transduction
      cell-cell signaling
      extracellular space
      negative regulation of angiogenesis
      activation of caspase activity
      immune response
      integral to plasma membrane
      positive regulation of I-kappaB kinase/NF-kappaB cascade
      induction of necroptosis of activated-T cells
      necrotic cell death
      activation of pro-apoptotic gene products
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoprecipitation
      Western Blotting
      Flow Cytometry(1)1/251/50
      (1)
      Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoprecipitation
      Western Blotting
      Flow Cytometry(1)1/251/50
      (1)
      Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoprecipitation
      Western Blotting
      Flow Cytometry(1)1/251/50
      (1)
      Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Results maybe enhanced using membrane permeabilisation. Bio-Rad recommends the use of LeucopermTM (Product code BUF09) prior to staining.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD178 Antibody Formats

    Formats Clone Applications Sizes available
    CD178 Antibody : Alexa Fluor® 488 14C2 F * 100 Tests/1ml | 25 Tests/0.25ml
    CD178 Antibody : RPE 14C2 F * 100 Tests | 25 Tests/0.25ml
    CD178 Antibody : Alexa Fluor® 647 14C2 F * 100 Tests/1ml | 25 Tests/0.25ml
    CD178 Antibody : Purified 14C2 E, F *, IP 0.2 mg | 25 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

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          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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