CD115 antibody | FER216
Mouse anti Human CD115 antibody, clone FER216 (MCA6111) used to examine CD115 expression in human samples by immunocytochemistry.
Image caption:
Immunohistochemistry analysis of CSF1R.
IHC staining of antibody FER216 labelled the HEK-CSFR1-Fc transfectants expressing CSFR1 but not the negative control HEK-FGFR1-Fc expressing Fc transfectants. The positive anti-Fc mAb control labelled both sets of transfectants.
From: Martín-Moreno AM, Roncador G, Maestre L, Mata E, Jiménez S, Martínez-Torrecuadrada JL, et al. (2015)
CSF1R Protein Expression in Reactive Lymphoid Tissues and Lymphoma: Its Relevance in Classical Hodgkin Lymphoma.
PLoS ONE 10(6): e0125203.
doi:10.1371/journal.pone.0125203
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD115 antibody, clone FER216 (MCA6111) used to examine CD115 expression in human samples by western blotting.
Image caption:
Western blot analysis of CSF1R.
Western blot analyses of CSF1R protein using protein extracts from HEK293 cells transfected with either CSF1R or a closely related protein (FGFR1) as a negative control, normal tissues, cHL tumour samples, and cHL-derived cell lines. Antibody FER216 recognised a band of 100 kDa in tonsil extracts and two bands of 100 and 150 kDa in extract from three cHL biopsies. Comparable results were obtained using the anti-CSFR1 mAb clone 61701. The mAb to tubulin was used as the loading control.
From: Martín-Moreno AM, Roncador G, Maestre L, Mata E, Jiménez S, Martínez-Torrecuadrada JL, et al. (2015)
CSF1R Protein Expression in Reactive Lymphoid Tissues and Lymphoma: Its Relevance in Classical Hodgkin Lymphoma.
PLoS ONE 10(6): e0125203.
doi:10.1371/journal.pone.0125203
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD115 antibody, clone FER216 (MCA6111) used to examine CD115 expression in human samples by immunoprecipitation and western blotting.
Image caption:
Immunoprecipitation and western blot analysis of CSF1R.
IP followed by western blotting demonstrated comparable results using either the FER216 or Clone 61701. Both antibodies were able to immunoprecipitate bands of 100 kDa from tonsil and 100 and 150 kDa from a case of cHL.
From: Martín-Moreno AM, Roncador G, Maestre L, Mata E, Jiménez S, Martínez-Torrecuadrada JL, et al. (2015)
CSF1R Protein Expression in Reactive Lymphoid Tissues and Lymphoma: Its Relevance in Classical Hodgkin Lymphoma.
PLoS ONE 10(6): e0125203.
doi:10.1371/journal.pone.0125203
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD115 antibody, clone FER216 (MCA6111) used to examine CD115 expression in human samples by immunofluorescence and immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical expression of CSF1R protein in normal lymphoid tissues.
CSF1R+ macrophages (brown) are abundant in the paracortex of the lymph nodes (A), the red pulp of the spleen (B), and the medulla of the thymus (C). Panels D-E, double immunofluorescence experiments in tonsil: CSF1R (red) and CD68 (green) (D) and CSF1R (red) and CD163 (green) (E). It can be seen that while CSFR1+ cells also express CD68 (D), CD163 is limited to only a subpopulation of CSFR1+ cells (E).
From: Martín-Moreno AM, Roncador G, Maestre L, Mata E, Jiménez S, Martínez-Torrecuadrada JL, et al. (2015)
CSF1R Protein Expression in Reactive Lymphoid Tissues and Lymphoma: Its Relevance in Classical Hodgkin Lymphoma.
PLoS ONE 10(6): e0125203.
doi:10.1371/journal.pone.0125203
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD115 antibody, clone FER216 (MCA6111) used to examine CD115 expression in human samples by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical detection of CSF1R protein in different lymphoma types.
CSF1R+ cells are less abundant in B-CLL and B- and T-LBL, and more frequent in the microenvironment of T-cell lymphomas (PTCL, AITL, and ALCL), and cHL.
From: Martín-Moreno AM, Roncador G, Maestre L, Mata E, Jiménez S, Martínez-Torrecuadrada JL, et al. (2015)
CSF1R Protein Expression in Reactive Lymphoid Tissues and Lymphoma: Its Relevance in Classical Hodgkin Lymphoma.
PLoS ONE 10(6): e0125203.
doi:10.1371/journal.pone.0125203
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD115 antibody, clone FER216 (MCA6111) used for the detection of human CSF1R expressing cells in tonsils by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical expression of CSF1R in reactive tonsils.
The CSF1R expression was characteristic of macrophages. Their distribution was mainly interfollicular. In the germinal centers, a weak expression could be found in the tangible body macrophages. The B-lymphocytes were negative for CSF1R.
From: Carreras J, Kikuti YY, Miyaoka M, Roncador G, Garcia JF, Hiraiwa S, Tomita S, Ikoma H, Kondo Y, Ito A, Komohara Y, Nakamura N, Hamoudi R.
Integrative Statistics, Machine Learning and Artificial Intelligence Neural Network Analysis Correlated CSF1R with the Prognosis of Diffuse Large B-Cell Lymphoma.
Hemato. 2021; 2(2):182-206.
doi: 10.3390/hemato2020011
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD115 antibody, clone FER216 (MCA6111) used for the detection of human CSF1R expressing cells in human tissue samples by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Quantification of Macrophage colony-stimulating factor 1 receptor (CSF1R) in the samples of Tokai University. 1
In diffuse large B-cell lymphoma (DLBCL), the CSF1R expression was characteristic of tumor-associated macrophages (TAMs). In addition, a CSF1R-positive B-cells pattern was also found.
From: Carreras J, Kikuti YY, Miyaoka M, Roncador G, Garcia JF, Hiraiwa S, Tomita S, Ikoma H, Kondo Y, Ito A, Komohara Y, Nakamura N, Hamoudi R.
Integrative Statistics, Machine Learning and Artificial Intelligence Neural Network Analysis Correlated CSF1R with the Prognosis of Diffuse Large B-Cell Lymphoma.
Hemato. 2021; 2(2):182-206.
doi: 10.3390/hemato2020011
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD115 antibody, clone FER216 (MCA6111) used for the detection of human CSF1R expressing cells in human tissue samples by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Quantification of Macrophage colony-stimulating factor 1 receptor (CSF1R) in the samples of Tokai University. 2
In diffuse large B-cell lymphoma (DLBCL), the CSF1R expression was characteristic of tumor-associated macrophages (TAMs). In addition, a CSF1R-positive B-cells pattern was also found.
From: Carreras J, Kikuti YY, Miyaoka M, Roncador G, Garcia JF, Hiraiwa S, Tomita S, Ikoma H, Kondo Y, Ito A, Komohara Y, Nakamura N, Hamoudi R.
Integrative Statistics, Machine Learning and Artificial Intelligence Neural Network Analysis Correlated CSF1R with the Prognosis of Diffuse Large B-Cell Lymphoma.
Hemato. 2021; 2(2):182-206.
doi: 10.3390/hemato2020011
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD115 antibody, clone FER216 recognizes the human CD115 cell surface antigen, also known as M-CSF receptor and c-fms. CD115 plays an important part in regulating the function, differentiation and proliferation of various cell types, with the major site of expression being macrophages. Human CD115 is a 972 amino acid 108 kDa single pass type I transmembrane glycoprotein however as the protein can be heavily modified by post translational modifications, the molecular weight of the receptor can vary significantly.
The receptor has tyrosine kinase activity and acts as a membrane receptor for M-CSF and IL-34. Activation induces homodimerization, phosphorylation and ubiquitination of intracellular residues triggering kinase signaling pathways.
CD115 chromosomal and expression abnormalities are involved in the development of Leukoencephalopathy, diffuse hereditary, with spheroids (HDLS), a adult-onset rapidly progressive neurodegenerative disorder demonstrating a variety of behavioural, cognitive and motor symptoms. CD115 mutations are also associated with Brain abnormalities, neurodegeneration, and dysosteosclerosis (BANDDOS) as well as other pathologies including neoplastic, inflammatory and neurological diseases (Chitu et al. 2016).
The receptor has tyrosine kinase activity and acts as a membrane receptor for M-CSF and IL-34. Activation induces homodimerization, phosphorylation and ubiquitination of intracellular residues triggering kinase signaling pathways.
CD115 chromosomal and expression abnormalities are involved in the development of Leukoencephalopathy, diffuse hereditary, with spheroids (HDLS), a adult-onset rapidly progressive neurodegenerative disorder demonstrating a variety of behavioural, cognitive and motor symptoms. CD115 mutations are also associated with Brain abnormalities, neurodegeneration, and dysosteosclerosis (BANDDOS) as well as other pathologies including neoplastic, inflammatory and neurological diseases (Chitu et al. 2016).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant protein of the extracellular domain of CSF1R (NP_005202.2, residues 1-514)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P07333
- Entrez Gene
- CSF1R
- GO Terms
- GO:0005011 macrophage colony-stimulating factor receptor activity
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005887 integral to plasma membrane
- GO:0005524 ATP binding
- GO:0006468 protein phosphorylation
- GO:0007275 multicellular organismal development
- GO:0008283 cell proliferation
- GO:0019955 cytokine binding
- GO:0042803 protein homodimerization activity
- GO:0043235 receptor complex
- Regulatory
- For research purposes only
Applications of CD115 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/200 | |
| Immunohistology - Frozen | 1/10 | 1/50 | |
| Immunohistology - Paraffin | 1/10 | 1/50 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
Source Reference
-
Martín-Moreno, A.M. et al. (2015) CSF1R Protein Expression in Reactive Lymphoid Tissues and Lymphoma: Its Relevance in Classical Hodgkin Lymphoma.
PLoS One. 10 (6): e0125203.
References for CD115 antibody
-
Carreras, J. et al. (2021) Integrative Statistics, Machine Learning and Artificial Intelligence Neural Network Analysis Correlated CSF1R with the Prognosis of Diffuse Large B-Cell Lymphoma
Hemato. 2 (2): 182-206.
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