CD115 antibody | FER216

Mouse anti Human CD115/CSF1R

Product Type
Monoclonal Antibody
Clone
FER216
Isotype
IgG1
Specificity
CD115

Product Code Applications Pack Size List Price Your Price Qty
MCA6111
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SDS Safety Datasheet SDS
C F P 0.1 mg loader
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loader
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Mouse anti Human CD115 antibody, clone FER216 recognizes the human CD115 cell surface antigen, also known as M-CSF receptor and c-fms. CD115 plays an important part in regulating the function, differentiation and proliferation of various cell types, with the major site of expression being macrophages. Human CD115 is a 972 amino acid 108 kDa single pass type I transmembrane glycoprotein however as the protein can be heavily modified by post translational modifications, the molecular weight of the receptor can vary significantly.

The receptor has tyrosine kinase activity and acts as a membrane receptor for M-CSF and IL-34. Activation induces homodimerization, phosphorylation and ubiquitination of intracellular residues triggering kinase signaling pathways.

CD115 chromosomal and expression abnormalities are involved in the development of Leukoencephalopathy, diffuse hereditary, with spheroids (HDLS), a adult-onset rapidly progressive neurodegenerative disorder demonstrating a variety of behavioural, cognitive and motor symptoms. CD115 mutations are also associated with Brain abnormalities, neurodegeneration, and dysosteosclerosis (BANDDOS) as well as other pathologies including neoplastic, inflammatory and neurological diseases (Chitu et al. 2016).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant protein of the extracellular domain of CSF1R (NP_005202.2, residues 1-514)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen 1/10 1/50
Immunohistology - Paraffin 1/10 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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Source Reference

  1. Martín-Moreno, A.M. et al. (2015) CSF1R Protein Expression in Reactive Lymphoid Tissues and Lymphoma: Its Relevance in Classical Hodgkin Lymphoma.
    PLoS One. 10 (6): e0125203.

References for CD115 antibody

  1. Carreras, J. et al. (2021) Integrative Statistics, Machine Learning and Artificial Intelligence Neural Network Analysis Correlated CSF1R with the Prognosis of Diffuse Large B-Cell Lymphoma
    Hemato. 2 (2): 182-206.

Synonyms
c-fms
UniProt
P07333
Entrez Gene
CSF1R
GO Terms
GO:0005011 macrophage colony-stimulating factor receptor activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0007275 multicellular organismal development
GO:0008283 cell proliferation
GO:0019955 cytokine binding
GO:0042803 protein homodimerization activity
GO:0043235 receptor complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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