IgG1 antibody

Goat anti Mouse IgG1:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG1

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STAR132F
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Goat anti Mouse IgG1 antibody recognizes Mouse IgG1. This antibody has been cross absorbed against mouse IgM, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Goat anti Mouse IgG1 antibody shows minimal cross-reactivity with human immunoglobulins.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to mouse IgG1 were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Mouse IgG1 paraproteins.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/1000
Immunofluorescence Neat 1/10
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

References for IgG1 antibody

  1. Croft, N.P. et al. (2009) Stage-specific inhibition of MHC class I presentation by the Epstein-Barr virus BNLF2a protein during virus lytic cycle.
    PLoS Pathog. 5(6): e1000490.
  2. Zuo, J. et al. (2011) The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting MHC class I molecules trafficking on both the exocytic and endocytic pathways.
    J Virol. 85: 1604-14.
  3. Knipping, K. et al. (2011) A gastrointestinal rotavirus infection mouse model for immune modulation studies.
    Virol J. 8: 109.
  4. Young, D. et al. (2012) Soy-derived di- and tripeptides alleviate colon and ileum inflammation in pigs with dextran sodium sulfate-induced colitis.
    J Nutr. 142 (2): 363-8.
  5. Bagai, U. and Pawar, A. (2013) A blood stage fraction of Plasmodium berghei induces protective and long lasting immune response in BALB/c mice.
    Parasitol Int. 62: 329-36.
  6. Anda, S. et al. (2014) Cell-cycle analyses using thymidine analogues in fission yeast.
    PLoS One. 9 (2): e88629.
  7. Kamat, M.M. et al. (2016) Changes in Myeloid Lineage Cells in the Uterus and Peripheral Blood of Dairy Heifers During Early Pregnancy.
    Biol Reprod. Aug 10. pii: biolreprod.116.141069. [Epub ahead of print]
  8. Ramanathan, R. et al. (2015) Transplantation of human stem cell-derived hepatocytes in an animal model of acute liver failure.
    Surgery. 158 (2): 349-59.
  9. View The Latest Product References
  10. Hwang, S.R. et al. (2015) Altered expression levels of neurodevelopmental proteins in fetal brains of BTBR T+tf/J mice with autism-like behavioral characteristics.
    J Toxicol Environ Health A. 78 (8): 516-23.
  11. Zhao, Z. et al. (2015) Multiple B-cell epitope vaccine induces a Staphylococcus enterotoxin B-specific IgG1 protective response against MRSA infection.
    Sci Rep. 5: 12371.
  12. Gutiérrez-Miranda, B. et al. (2020) Oleacein Attenuates the Pathogenesis of Experimental Autoimmune Encephalomyelitis through Both Antioxidant and Anti-Inflammatory Effects.
    Antioxidants (Basel). 9 (11)Nov 21 [Epub ahead of print].
  13. Gutierrez, B. et al. (2020) Oleanolic acid ameliorates intestinal alterations associated with EAE
    Journal of Neuroinflammation. 17 (1) [Epub ahead of print].
  14. Apóstolo, N. et al. (2020) Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer.
    Nat Commun. 11 (1): 5171.
  15. Zhuang, X. et al. (2020) CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth.
    JCI Insight. 5 (19)Oct 02 [Epub ahead of print].
  16. Sparks, A.M. et al. (2018) Natural Selection on Antihelminth Antibodies in a Wild Mammal Population.
    Am Nat. 192 (6): 745-760.
  17. Kushwaha, V. et al. (2019) Troponin 1 of human filarial parasite Brugia malayi.: cDNA cloning, expression, purification, and its immunoprophylactic potential.
    Parasitol Res. 118 (6): 1849-63.
  18. Kim, Y.H. et al. (2019) Effects of ruminal pH on gene expression in the rumen epithelium, peripheral blood mononuclear cell subpopulations, and blood metabolites from Holstein calves during weaning transition.
    J Vet Med Sci. 81 (6): 808-816.
  19. Jimbo, S. et al. (2019) Natural and inducible regulatory B cells are widely distributed in ovine lymphoid tissues.
    Vet Immunol Immunopathol. 211: 44-48.
  20. Sparks, A.M. et al. (2019) The genetic architecture of helminth-specific immune responses in a wild population of Soay sheep (Ovis aries.).
    PLoS Genet. 15 (11): e1008461.
  21. Nedumpun, T. et al. (2019) Negative Immunomodulatory Effects of Type 2 Porcine Reproductive and Respiratory Syndrome Virus-Induced Interleukin-1 Receptor Antagonist on Porcine Innate and Adaptive Immune Functions.
    Front Immunol. 10: 579.
  22. Verma, R. et al. (2018) Leishmania donovani. molecules recognized by sera of filaria infected host facilitate filarial infection.
    Parasitol Res. 117 (9): 2901-12.
  23. Gatkowska, J. et al. (2019) The Impact of the Antigenic Composition of Chimeric Proteins on Their Immunoprotective Activity against Chronic Toxoplasmosis in Mice.
    Vaccines (Basel). 7(4):154.
  24. Han, H. et al. (2021) Metal arsenic mediated enhancement of type-2 immunity in brains with altered locomotive activities in mice with autism-like behavioral characteristics
    Toxicological Research. 08 Oct [Epub ahead of print].
  25. Anda, S. et al. (2021) Cosegregation of asymmetric features during cell division.
    Open Biol. 11 (8): 210116.
  26. Minaei, S. et al. (2018) Propranolol efficacy as a novel adjuvant for immunization against Toxoplasma gondii. tachyzoites.
    Exp Parasitol. 194: 60-66.

ELISA

Flow Cytometry

RRID
AB_2124271
UniProt
P01869
P01868
Entrez Gene
Ighg1
Ighg1
GO Terms
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0003823 antigen binding
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0050766 positive regulation of phagocytosis
GO:0005886 plasma membrane
GO:0016021 integral to membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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