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Calcitonin Receptor antibody | 31/01-1H10-4-1-14

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Anti Human Calcitonin Receptor Antibody, clone 31/01-1H10-4-1-14 thumbnail image 1 Anti Human Calcitonin Receptor Antibody, clone 31/01-1H10-4-1-14 thumbnail image 2 Anti Human Calcitonin Receptor Antibody, clone 31/01-1H10-4-1-14 thumbnail image 3
Anti Human Calcitonin Receptor Antibody, clone 31/01-1H10-4-1-14 gallery image 1
Published customer image:
Mouse anti Human Calcitonin receptor antibody, clone 31/01-1H10-4-1-14 (MCA2191) used for the demonstration of CTR expression in glioblastoma cell line lysates by western blotting.
Image caption:
Western blot for CTR, 50 μg of membrane protein probed with anti-CTR 1H10, representative of 3 independent experiments.

From: Ostrovskaya A, Hick C, Hutchinson DS, Stringer BW, Wookey PJ, Wootten D, Sexton PM, Furness SGB.
Expression and activity of the calcitonin receptor family in a sample of primary human high-grade gliomas.
BMC Cancer. 2019 Feb 18;19(1):157.
doi: 10.1186/s12885-019-5369-y.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Calcitonin Receptor Antibody, clone 31/01-1H10-4-1-14 gallery image 2
Published customer image:
Mouse anti Human calcitonin receptor antibody, clone 31/01-1H10-4-1-14 (MCA2191) used for the demonstration of CTR expressing cells in medullary thyroid carcinoma tissue sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
CTR expression in MTC.
(A and B) negative control, a MTC case in which the primary antibody was omitted. (C and D) negative CTR-expression in a case of MTC. (E and F) positive CTR-expression in a case of MTC (score 2: extent 25–50%, intensity 1+). (G and H) positive CTR-expression in a case of MTC (score 12: extent 100%, intensity 3+). The dashed square in (A), (B) and (C) photomicrographs taken at 10× magnification represents the area in (D), (E) and (F) pictures taken using the 60× objective. Bar 100 μm.

From: Cappagli V, Potes CS, Ferreira LB, Tavares C, Eloy C, Elisei R, Sobrinho-Simões M, Wookey PJ, Soares P.
Calcitonin receptor expression in medullary thyroid carcinoma.
PeerJ. 2017 Sep 13;5:e3778.
doi: 10.7717/peerj.3778.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Calcitonin Receptor Antibody, clone 31/01-1H10-4-1-14 gallery image 3
Published customer image:
Mouse anti Human calcitonin receptor antibody, clone 31/01-1H10-4-1-14 (MCA2191) used for the demonstration of CTR expressing cells in medullary thyroid carcinoma tissue sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
CT and CTR staining in serial MTC tissue sections.
(A and D) CTR score 0 and CT intensity 1. (B and E) CTR score 4 (extent 75–100%, intensity 1), CT intensity 3. (C and F) CTR score 12 (extent 75–100%, intensity 3), CT intensity 4. Note that higher CTR scores correspond to more intense CT stainings. Photomicrographs were taken at 10× magnification. Bar 100μm.

From: Cappagli V, Potes CS, Ferreira LB, Tavares C, Eloy C, Elisei R, Sobrinho-Simões M, Wookey PJ, Soares P.
Calcitonin receptor expression in medullary thyroid carcinoma.
PeerJ. 2017 Sep 13;5:e3778.
doi: 10.7717/peerj.3778.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human Calcitonin Receptor

Product Type
Monoclonal Antibody
Clone
31/01-1H10-4-1-14
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2191
Datasheet
C E F IF P* WB
SDS
0.1 mg loader

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P30988
Entrez Gene
CALCR
GO Terms
GO:0005515 protein binding
GO:0004948 calcitonin receptor activity
GO:0005887 integral to plasma membrane
GO:0007189 activation of adenylate cyclase activity by G-protein signaling pathway
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0032841 calcitonin binding
GO:0045762 positive regulation of adenylate cyclase activity
GO:0051384 response to glucocorticoid stimulus
Regulatory
For research purposes only

Applications of Calcitonin Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/5000
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/50 1/500
Western Blotting
  1. 1Fixation with 4% paraformaldehyde is recommended for optimal results.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Kidney
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control MCA929 F 100 Tests loader

Product Specific References

References for Calcitonin Receptor antibody

  1. Wookey, P.J. et al. (2008) Calcitonin receptor immunoreactivity associated with specific cell types in diseased radial and internal mammary arteries.
    Histopathology. 52 (5): 605-12.
  2. Silvestris, F. et al. (2008) Expression and function of the calcitonin receptor by myeloma cells in their osteoclast-like activity in vitro.
    Leuk Res. 32 (4): 611-23.
  3. Wookey, P.J. et al. (2009) The elevated expression of calcitonin receptor by cells recruited into the endothelial layer and neo-intima of atherosclerotic plaque.
    Histochem Cell Biol. 132: 181-9.
  4. Wookey, P.J. et al. (2012) The expression of calcitonin receptor detected in malignant cells of the brain tumour glioblastoma multiforme and functional properties in the cell line A172.
    Histopathology. 60 (6): 895-910.
  5. Lesclous, P. et al. (2009) Bisphosphonate-associated osteonecrosis of the jaw: a key role of inflammation?
    Bone. 45 (5): 843-52.
  6. Ostrovskaya, A. et al. (2019) Expression and activity of the calcitonin receptor family in a sample of primary human high-grade gliomas.
    BMC Cancer. 19 (1): 157.
  7. Cappagli, V. et al. (2017) Calcitonin receptor expression in medullary thyroid carcinoma.
    PeerJ. 5: e3778.

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