Calcitonin Receptor antibody | 31/01-1H10-4-1-14
Mouse anti Human Calcitonin receptor antibody, clone 31/01-1H10-4-1-14 (MCA2191) used for the demonstration of CTR expression in glioblastoma cell line lysates by western blotting.
Image caption:
Western blot for CTR, 50 μg of membrane protein probed with anti-CTR 1H10, representative of 3 independent experiments.
From: Ostrovskaya A, Hick C, Hutchinson DS, Stringer BW, Wookey PJ, Wootten D, Sexton PM, Furness SGB.
Expression and activity of the calcitonin receptor family in a sample of primary human high-grade gliomas.
BMC Cancer. 2019 Feb 18;19(1):157.
doi: 10.1186/s12885-019-5369-y.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human calcitonin receptor antibody, clone 31/01-1H10-4-1-14 (MCA2191) used for the demonstration of CTR expressing cells in medullary thyroid carcinoma tissue sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
CT and CTR staining in serial MTC tissue sections.
(A and D) CTR score 0 and CT intensity 1. (B and E) CTR score 4 (extent 75–100%, intensity 1), CT intensity 3. (C and F) CTR score 12 (extent 75–100%, intensity 3), CT intensity 4. Note that higher CTR scores correspond to more intense CT stainings. Photomicrographs were taken at 10× magnification. Bar 100μm.
From: Cappagli V, Potes CS, Ferreira LB, Tavares C, Eloy C, Elisei R, Sobrinho-Simões M, Wookey PJ, Soares P.
Calcitonin receptor expression in medullary thyroid carcinoma.
PeerJ. 2017 Sep 13;5:e3778.
doi: 10.7717/peerj.3778.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human calcitonin receptor antibody, clone 31/01-1H10-4-1-14 (MCA2191) used for the demonstration of CTR expressing cells in medullary thyroid carcinoma tissue sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
CTR expression in MTC.
(A and B) negative control, a MTC case in which the primary antibody was omitted. (C and D) negative CTR-expression in a case of MTC. (E and F) positive CTR-expression in a case of MTC (score 2: extent 25–50%, intensity 1+). (G and H) positive CTR-expression in a case of MTC (score 12: extent 100%, intensity 3+). The dashed square in (A), (B) and (C) photomicrographs taken at 10× magnification represents the area in (D), (E) and (F) pictures taken using the 60× objective. Bar 100 μm.
From: Cappagli V, Potes CS, Ferreira LB, Tavares C, Eloy C, Elisei R, Sobrinho-Simões M, Wookey PJ, Soares P.
Calcitonin receptor expression in medullary thyroid carcinoma.
PeerJ. 2017 Sep 13;5:e3778.
doi: 10.7717/peerj.3778.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human calcitonin recptor antibody, clone 31/01-1H10-4-1-14 recognizes the human calcitonin receptor, a membrane protein with seven transmembrane domains that is coupled to G protein messenger systems. Mouse anti Human calcitonin recptor antibody, clone 31/01-1H10-4-1-14 recognizes an epitope within the cytoplasmic domain that is common to both C1a and C1b human isoforms.
Calcitonin receptor is expressed in kidney, central and peripheral nervous systems and on osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis.
Calcitonin receptor is expressed in kidney, central and peripheral nervous systems and on osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide, DIPIYICHQELRNEPANN, derived from the cytoplasmic domain of human calcitonin receptor.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P30988
- Entrez Gene
- CALCR
- GO Terms
- GO:0005515 protein binding
- GO:0004948 calcitonin receptor activity
- GO:0005887 integral to plasma membrane
- GO:0007189 activation of adenylate cyclase activity by G-protein signaling pathway
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0032841 calcitonin binding
- GO:0045762 positive regulation of adenylate cyclase activity
- GO:0051384 response to glucocorticoid stimulus
- Regulatory
- For research purposes only
Applications of Calcitonin Receptor antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | 1/5000 | |
| Flow Cytometry | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | 1/50 | 1/500 | |
| Western Blotting |
- 1Fixation with 4% paraformaldehyde is recommended for optimal results.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Product Specific References
References for Calcitonin Receptor antibody
-
Wookey, P.J. et al. (2008) Calcitonin receptor immunoreactivity associated with specific cell types in diseased radial and internal mammary arteries.
Histopathology. 52 (5): 605-12. -
Silvestris, F. et al. (2008) Expression and function of the calcitonin receptor by myeloma cells in their osteoclast-like activity in vitro.
Leuk Res. 32 (4): 611-23. -
Wookey, P.J. et al. (2009) The elevated expression of calcitonin receptor by cells recruited into the endothelial layer and neo-intima of atherosclerotic plaque.
Histochem Cell Biol. 132: 181-9. -
Wookey, P.J. et al. (2012) The expression of calcitonin receptor detected in malignant cells of the brain tumour glioblastoma multiforme and functional properties in the cell line A172.
Histopathology. 60 (6): 895-910. -
Lesclous, P. et al. (2009) Bisphosphonate-associated osteonecrosis of the jaw: a key role of inflammation?
Bone. 45 (5): 843-52. -
Ostrovskaya, A. et al. (2019) Expression and activity of the calcitonin receptor family in a sample of primary human high-grade gliomas.
BMC Cancer. 19 (1): 157. -
Cappagli, V. et al. (2017) Calcitonin receptor expression in medullary thyroid carcinoma.
PeerJ. 5: e3778.
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