Calcitonin Receptor antibody | 31/01-1H10-4-1-14

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Mouse anti Human Calcitonin Receptor

Product Type
Monoclonal Antibody
Clone
31/01-1H10-4-1-14
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2191 C E F IF P* WB 0.1 mg
Mouse anti Human calcitonin recptor antibody, clone 31/01-1H10-4-1-14 recognizes the human calcitonin receptor, a membrane protein with seven transmembrane domains that is coupled to G protein messenger systems. Mouse anti Human calcitonin recptor antibody, clone 31/01-1H10-4-1-14 recognizes an epitope within the cytoplasmic domain that is common to both C1a and C1b human isoforms.

Calcitonin receptor is expressed in kidney, central and peripheral nervous systems and on osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Synthetic peptide, DIPIYICHQELRNEPANN, derived from the cytoplasmic domain of human calcitonin receptor.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P30988 Related reagents
Entrez Gene
CALCR Related reagents
GO Terms
GO:0005515 protein binding
GO:0004948 calcitonin receptor activity
GO:0005887 integral to plasma membrane
GO:0007189 activation of adenylate cyclase activity by G-protein signaling pathway
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0032841 calcitonin binding
GO:0045762 positive regulation of adenylate cyclase activity
GO:0051384 response to glucocorticoid stimulus
Regulatory
For research purposes only

Applications of Calcitonin Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/5000
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/50 1/500
Western Blotting
  1. 1Fixation with 4% paraformaldehyde is recommended for optimal results.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Kidney

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA929 100 Tests F

Product Specific References

References for Calcitonin Receptor antibody

  1. Wookey, P.J. et al. (2008) Calcitonin receptor immunoreactivity associated with specific cell types in diseased radial and internal mammary arteries.
    Histopathology. 52 (5): 605-12.
  2. Silvestris, F. et al. (2008) Expression and function of the calcitonin receptor by myeloma cells in their osteoclast-like activity in vitro.
    Leuk Res. 32 (4): 611-23.
  3. Wookey, P.J. et al. (2009) The elevated expression of calcitonin receptor by cells recruited into the endothelial layer and neo-intima of atherosclerotic plaque.
    Histochem Cell Biol. 132: 181-9.
  4. Wookey, P.J. et al. (2012) The expression of calcitonin receptor detected in malignant cells of the brain tumour glioblastoma multiforme and functional properties in the cell line A172.
    Histopathology. 60 (6): 895-910.
  5. Lesclous, P. et al. (2009) Bisphosphonate-associated osteonecrosis of the jaw: a key role of inflammation?
    Bone. 45 (5): 843-52.