Actin Gamma antibody | 2A3

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Mouse anti Human Actin Gamma

Product Type
Monoclonal Antibody
Clone
2A3
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5776GA IF P WB 0.1 mg
Mouse anti Human actin gamma antibody, clone 2A3 recognises cytoplasmic actin gamma (known also as ACTG and Gamma Actin), a 41.8 kDa cytoskeletal protein.

Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified (Vandekerckhove and Weber 1978) .

Clone 2A3 is highly specific for actin gamma and does not react with other actin isoforms (Dugina et al. 2009).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals Expected from Sequence
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Synthetic peptide corresponding to the N-terminal nonapeptide of gamma-cytoplasmic actin, coupled to KLH.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P63261 Related reagents
Entrez Gene
ACTG1 Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005200 structural constituent of cytoskeleton
GO:0005524 ATP binding
GO:0005856 cytoskeleton
GO:0006928 cellular component movement
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
Regulatory
For research purposes only

Applications of Actin Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Paraffin
Western Blotting 1/100 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for Actin Gamma antibody

  1. Dugina, V. et al. (2009) Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity.
    J Cell Sci. 122: 2980-8.
  2. Brockmann, C. et al. (2011) Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes.
    Biol Reprod. 85: 1025-39.
  3. Miazza, V. et al. (2011) Sendai virus induced cytoplasmic actin remodeling correlates with efficient virus particle production.
    Virology. 410: 7-16.
  4. Baranwal, S. et al. (2012) Nonredundant roles of cytoplasmic β- and γ-actin isoforms in regulation of epithelial apical junctions.
    Mol Biol Cell. 23: 3542-53.
  5. Dugina, V. et al. (2015) Tumor promotion by γ and suppression by β non-muscle actin isoforms.
    Oncotarget. 6 (16): 14556-71.
  6. Simiczyjew A et al. (2015) Active invadopodia of mesenchymally migrating cancer cells contain both β and γ cytoplasmic actin isoforms.
    Exp Cell Res. pii: S0014-4827(15)30146-4.
  7. Simiczyjew, A. et al. (2017) Involvement of β- and γ-actin isoforms in actin cytoskeleton organization and migration abilities of bleb-forming human colon cancer cells.
    PLoS One. 12 (3): e0173709.
  8. Chaponnier, C. & Gabbiani, G. (2016) Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
    F1000Res. 5: 416.
  9. Chen, A. et al. (2017) Cytokinesis requires localized β-actin filament production by an actin isoform specific nucleator.
    Nat Commun. 8 (1): 1530.