Actin Gamma antibody | 2A3
Mouse anti Human actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in normal and neoplasic cells by immunofluorescence and western blotting.
Image caption:
Expression of cytoplasmic actins in normal and transformed cells (A-C); expression of cytoplasmic actins and HaCaT cellular characteristics (D-G) A. Immunofluorescent staining for β-actin (green) and γ-actin (red) of representative pairs of NSCLC and Colon Cancer samples with matching normal tissue samples. Scale bars represent 50μm. Graphs represent staining intensity of biopsies (Mean ± SD). B. WB analysis of HaCaT cells with exogenous expression of active N-RasD13. Graphs represent relative actins expression (Mean ± SD). C. Immunofluorescent staining for β-actin (green) and γ-actin (red) of N-RasD13 transformed HaCaT cells. Scale bars represent 10 μm. D. WB analysis of HaCaT cells with exogenous expression of β- or γ-actins and corresponding shRNAs. shC is sh to GFP. Graphs represent relative actins expression (Mean ± SD). E. Semi-quantitative RT-PCR analysis of β- or γ-actins expression in cells with corresponding shRNAs. Graphs represent folds of RNA expression in comparison to control cells (Mean ± SD). F. Immunofluorescent staining of HaCaT cells with altered β- or γ-actins expression. Scale bars represent 10 μm. G. HaCaT cells with silenced or exogenously expressed non-muscle cytoplasmic actins that crossed the matrigel-coated membranes (left). Graphs represent mean ± SD. HaCaT proliferation dynamics with exogenous expression or silenced of β- or γ-actins (right). Error bars are SD.
From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.
Mouse anti Human actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in cell lines by immunofluorescence and western blotting.
Image caption:
β- and γ-actins in neoplastic cells phenotype A. WB analysis of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative actins expression (Mean ± SD). B. Semi-quantitative RT-PCR analysis of β- or γ-actins expression in A549 (left panel) and HCT116 (right panel) with corresponding shRNAs. Graphs represent folds of RNA expression in comparison to control cells (Mean ± SD). C. Immunofluorescent staining of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Scale bars represent 10 μm.
From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.
Mouse anti Human actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in cell lines by immunofluorescence.
Image caption:
β- and γ-actins in neoplastic cells phenotype A. WB analysis of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative actins expression (Mean ± SD). B. Semi-quantitative RT-PCR analysis of β- or γ-actins expression in A549 (left panel) and HCT116 (right panel) with corresponding shRNAs. Graphs represent folds of RNA expression in comparison to control cells (Mean ± SD). C. Immunofluorescent staining of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Scale bars represent 10 μm.
From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.
Mouse anti Human actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.
Image caption:
γ-actin and ERK1/2 are mutually regulated A. WB analysis of A549 cells with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative pERK1/2 expression (Mean ± SD). B. LSM of A549 cells with down-regulated β- or γ-actins with β-actin (green), γ-actin (purple) or pERK1/2 (red) immunofluorescent staining. Scale bars represent 10 μm. C. pERK1/2 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. pERK1/2/γ-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. Graph represents relative fluorescence intensity (Mean ± SD). E. LSM of A549 cells treated with EGF or UO126 with β-actin (green) or γ-actin (red) immunofluorescent staining. Scale bars represent 10 μm. F. WB analysis of A549 cells treated with EGF or UO126. Graphs represent relative pERK1/2 or β-/γ-actin levels (Mean ± SD).
From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.
Mouse anti Human actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.
Image caption:
γ-actin co-localizes with p34-Arc and WAVE2 and regulates them A. p34-Arc (red) or γ-actin (green) immunofluorescence images of A549 cells with down-regulated β- or γ-actins. Scale bars represent 10 μm. B. γ-Actin (red) or WAVE2 (green) immunofluorescence images of A549 cells with down-regulated β- or γ-actins. Scale bars represent 10 μm. C. WB analysis of A549 cells with down-regulated β- or γ-actins. Graphs represent relative protein levels (Mean ± SD). D. p34-Arc immunoprecipitation analysis of A549 cells with down-regulated β- or &gamma-actins. E. WAVE2 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. F. LSM of A549 cells with down-regulated β-actin with p34-Arc (green, upper panel), WAVE2 (green, lower panel), β-actin (red) or γ-actin (purple) immunofluorescent staining. Scale bars represent 2 μm. Asterisk marks the cell with silenced β-actin.
From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.
Mouse anti Human actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.
Image caption:
γ-actin co-localizes with cofilin1 and regulates it A. LSM of A549 cells with down-regulated β- or γ-actins with β-actin (green), γ-actin (purple), cofilin1 (red) or DAPI (blue) immunofluorescent staining. Scale bars represent 10 μm. B. WB analysis of A549 cells with down-regulated β- or γ-actins. Graphs represent relative protein levels (Mean ± SD). C. Cofilin1 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. Cofilin1/γ-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. Graph represents relative fluorescence intensity (Mean ± SD).
From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.
Mouse anti Human actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.
Image caption:
γ-actin co-localizes with PP1 and regulates it A. LSM of A549 cells with down-regulated β- or γ-actins with β-actin (green), γ-actin (purple), PP1α (red) or DAPI (blue) immunofluorescent staining. Scale bars represent 10 μm. B. WB analysis of A549 cells with down-regulated β- or γ-actins. Graphs represent relative PP1&alpha levels (Mean ± SD). C. PP1 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. PP1α/γ-actin (upper panel) and PP1α/β-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. E. Comparative quantification of PP1α−β-/γ-actin PLA dots. Graph represents relative fluorescence intensity (Mean ± SD).
From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.
Mouse anti Human actin gamma; antibody, clone 2A3 (MCA5776GA) used for the evaluation of γ-actin expression in MDA-MB-231 cells by immunofluorescence and western blotting.
Image caption:
Downregulation of β- or γ-actin expression by corresponding shRNAs in MDA-MB-231 breast cancer cells leads to phenotype changes. (A). Immunofluorescent staining of MDA-MB-231 cells with β- or γ-actin downregulation by corresponding shRNAs. Arrowheads mark leading edge with actin enrichment. Bar, 10 μm. (B). Downregulation of cytoplasmic β- or γ-actin in MDA-MB-231 cells. WB analysis. Graphs represent relative actins expression (Mean ± SEM). For cells with shRNA compared with control (β-actin): p = 0.0015 (shRNA to β-actin); p = 0.007 (shRNA to γ-actin); (γ-actin): p = 0.0037 (shRNA to β-actin); p = 0.0048 (shRNA to γ-actin); Mann–Whitney U test, n = 3. Values of p <0.01 (**) were considered as statistically significant.
From: Dugina V, Shagieva G, Novikova M, Lavrushkina S, Sokova O, Kireev I, Kopnin P.
Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line.
Molecules. 2021; 26(8):2151.
doi: 10.3390/molecules26082151
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human actin gamma; antibody, clone 2A3 (MCA5776GA) used for the evaluation of γ-actin expression in MDA-MB-231 cells by immunofluorescence.
Image caption:
Visualization of pH3 histone in mitotic MDA-MB-231 control cells (metaphase, anaphase, telophase), with downregulated β-actin (prometa-metaphase) and with downregulated γ-actin (metaphase, anaphase, telophase). Immunofluorescence staining of H3 (green), γ-actin (red) and DNA (DAPI staining, blue). Bar 5 μm.
From: Dugina V, Shagieva G, Novikova M, Lavrushkina S, Sokova O, Kireev I, Kopnin P.
Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line.
Molecules. 2021; 26(8):2151.
doi: 10.3390/molecules26082151
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human actin gamma antibody, clone 2A3 recognizes cytoplasmic actin gamma (known also as ACTG and Gamma Actin), a 41.8 kDa cytoskeletal protein.
Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified (Vandekerckhove and Weber 1978) .
Mouse anti Human actin gamma antibody, clone 2A3 is highly specific for actin gamma and does not react with other actin isoforms (Dugina et al. 2009).
Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified (Vandekerckhove and Weber 1978) .
Mouse anti Human actin gamma antibody, clone 2A3 is highly specific for actin gamma and does not react with other actin isoforms (Dugina et al. 2009).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mammals Expected from Sequence Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Synthetic peptide corresponding to the N-terminal nonapeptide of gamma-cytoplasmic actin, coupled to KLH.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P63261
- Entrez Gene
- ACTG1
- GO Terms
- GO:0007596 blood coagulation
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0005524 ATP binding
- GO:0005856 cytoskeleton
- GO:0006928 cellular component movement
- GO:0007411 axon guidance
- GO:0042802 identical protein binding
- GO:0034329 cell junction assembly
- GO:0034332 adherens junction organization
- Regulatory
- For research purposes only
Applications of Actin Gamma antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | |||
| Immunohistology - Paraffin | |||
| Western Blotting | 1/100 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Actin Gamma antibody
-
Dugina, V. et al. (2009) Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity.
J Cell Sci. 122: 2980-8. -
Brockmann, C. et al. (2011) Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes.
Biol Reprod. 85: 1025-39. -
Miazza, V. et al. (2011) Sendai virus induced cytoplasmic actin remodeling correlates with efficient virus particle production.
Virology. 410: 7-16. -
Baranwal, S. et al. (2012) Nonredundant roles of cytoplasmic β- and γ-actin isoforms in regulation of epithelial apical junctions.
Mol Biol Cell. 23: 3542-53. -
Dugina, V. et al. (2015) Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 6 (16): 14556-71. -
Simiczyjew A et al. (2015) Active invadopodia of mesenchymally migrating cancer cells contain both β and γ cytoplasmic actin isoforms.
Exp Cell Res. pii: S0014-4827(15)30146-4. -
Simiczyjew, A. et al. (2017) Involvement of β- and γ-actin isoforms in actin cytoskeleton organization and migration abilities of bleb-forming human colon cancer cells.
PLoS One. 12 (3): e0173709. -
Chaponnier, C. & Gabbiani, G. (2016) Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
F1000Res. 5: 416. -
Chen, A. et al. (2017) Cytokinesis requires localized β-actin filament production by an actin isoform specific nucleator.
Nat Commun. 8 (1): 1530. -
Dugina, V. et al. (2021) Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line.
Molecules. 26(8):2151.
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