Actin Beta antibody | 4C2
Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals (Vandekerckhove et al. 1978).
Clone 4C2 is highly specific for cytoplasmic actin β and does not react with other actin isoforms. Mouse anti Human actin beta, clone 4C2 has been used to look at the remodeling of the actin cytoskeleton following sendai virus infection and the specific role of the viral M protein in actin remodeling (Miazza et al. 2011).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rabbit Pig Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Synthetic peptide corresponding to the acetylated N-terminal nonapeptide of beta-cytoplasmic actin, coupled to KLH.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
Applications of Actin Beta antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/50||1/100|
Secondary Antibodies Available
Product Specific References
References for Actin Beta antibody
Dugina, V. et al. (2009) Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity.
J Cell Sci. 122 (Pt 16): 2980-8.
Brockmann, C. et al. (2011) Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes.
Biol Reprod. 85 (5): 1025-39.
Miazza, V. et al. (2011) Sendai virus induced cytoplasmic actin remodeling correlates with efficient virus particle production.
Virology. 410 (1): 7-16.
Baranwal, S. et al. (2012) Nonredundant roles of cytoplasmic β- and γ-actin isoforms in regulation of epithelial apical junctions.
Mol Biol Cell. 23 (18): 3542-53.
Shagieva, G.S. et al. (2012) Actin isoforms and reorganization of adhesion junctions in epithelial-to-mesenchymal transition of cervical carcinoma cells.
Biochemistry (Mosc). 77 (11): 1266-76.
Dugina, V. et al. (2015) Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 6 (16): 14556-71.
Rubtsova, S.N. et al. (2015) A Novel Role of E-Cadherin-Based Adherens Junctions in Neoplastic Cell Dissemination.
PLoS One. 10 (7): e0133578.
Simiczyjew A et al. (2015) Active invadopodia of mesenchymally migrating cancer cells contain both β and γ cytoplasmic actin isoforms.
Exp Cell Res. pii: S0014-4827(15)30146-4.
Simiczyjew, A. et al. (2017) Involvement of β- and γ-actin isoforms in actin cytoskeleton organization and migration abilities of bleb-forming human colon cancer cells.
PLoS One. 12 (3): e0173709.
Novikova, M.V. et al. (2017) [A change in the expression of membrane-associated proteins and cytoplasmic actin isoforms in the progression of human colon tumors].
Arkh Patol. 79 (2): 15-21.
Chaponnier, C. & Gabbiani, G. (2016) Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
F1000Res. 5: 416.
Chen, A. et al. (2017) Cytokinesis requires localized β-actin filament production by an actin isoform specific nucleator.
Nat Commun. 8 (1): 1530.
Dugina, V. et al. (2021) Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line.
Perrin, B.J. & Ervasti, J.M. (2010) The actin gene family: function follows isoform.
Cytoskeleton (Hoboken). 67 (10): 630-4.
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