Actin Beta antibody | 4C2

100% Secure


Mouse anti Human Actin Beta

Product Type
Monoclonal Antibody
Clone
4C2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5775GA C IF WB 0.1 mg
Mouse anti Human actin beta, clone 4C2, recognises cytoplasmic actin beta (known also as ACTB and Beta Actin), a 41.7 kDa cytoskeletal protein.

Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals (Vandekerckhove et al. 1978).

Clone 4C2 is highly specific for cytoplasmic actin β and does not react with other actin isoforms. Mouse anti Human actin beta, clone 4C2 has been used to look at the remodeling of the actin cytoskeleton following sendai virus infection and the specific role of the viral M protein in actin remodelling (Miazza et al. 2011)

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Pig
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Synthetic peptide corresponding to the acetylated N-terminal nonapeptide of beta-cytoplasmic actin, coupled to KLH.
Approx. Protein Concentrations
IgG concentration 1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P60709 Related reagents
Entrez Gene
ACTB Related reagents
Regulatory
For research purposes only

Applications of Actin Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen 1/50 1/100
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for Actin Beta antibody

  1. Dugina, V. et al. (2009) Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity.
    J Cell Sci. 122 (Pt 16): 2980-8.
  2. Brockmann, C. et al. (2011) Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes.
    Biol Reprod. 85 (5): 1025-39.
  3. Miazza, V. et al. (2011) Sendai virus induced cytoplasmic actin remodeling correlates with efficient virus particle production.
    Virology. 410 (1): 7-16.
  4. Baranwal, S. et al. (2012) Nonredundant roles of cytoplasmic β- and γ-actin isoforms in regulation of epithelial apical junctions.
    Mol Biol Cell. 23 (18): 3542-53.
  5. Shagieva, G.S. et al. (2012) Actin isoforms and reorganization of adhesion junctions in epithelial-to-mesenchymal transition of cervical carcinoma cells.
    Biochemistry (Mosc). 77 (11): 1266-76.
  6. Dugina, V. et al. (2015) Tumor promotion by γ and suppression by β non-muscle actin isoforms.
    Oncotarget. 6 (16): 14556-71.
  7. Rubtsova, S.N. et al. (2015) A Novel Role of E-Cadherin-Based Adherens Junctions in Neoplastic Cell Dissemination.
    PLoS One. 10 (7): e0133578.
  8. Simiczyjew A et al. (2015) Active invadopodia of mesenchymally migrating cancer cells contain both β and γ cytoplasmic actin isoforms.
    Exp Cell Res. pii: S0014-4827(15)30146-4.
  9. Simiczyjew, A. et al. (2017) Involvement of β- and γ-actin isoforms in actin cytoskeleton organization and migration abilities of bleb-forming human colon cancer cells.
    PLoS One. 12 (3): e0173709.
  10. Novikova, M.V. et al. (2017) [A change in the expression of membrane-associated proteins and cytoplasmic actin isoforms in the progression of human colon tumors].
    Arkh Patol. 79 (2): 15-21.
  11. Chaponnier, C. & Gabbiani, G. (2016) Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
    F1000Res. 5: 416.
  12. Chen, A. et al. (2017) Cytokinesis requires localized β-actin filament production by an actin isoform specific nucleator.
    Nat Commun. 8 (1): 1530.

Further Reading

  1. Perrin, B.J. & Ervasti, J.M. (2010) The actin gene family: function follows isoform.
    Cytoskeleton (Hoboken). 67 (10): 630-4.