Actin Beta Antibody | 12141

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Actin Beta Antibody | 12141 gallery image 1

Staining of J774 lysate with Human anti actin beta (HCA147) followed by Mouse anti Strep-tag HRP (MCA2489P)

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Actin Beta Antibody | 12141 gallery image 2

Staining of HeLa whole cell lysate with Human anti actin beta DyLight®800 (HCA147D800). Visualised using a LI-COR Odyssey® infrared imaging system

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Actin Beta Antibody | 12141 gallery image 3

Staining of mouse macrophage and fibroblast cell lysates with Human anti actin beta DyLight®680 (HCA147D680). Visualised using a LI-COR Odyssey® infrared imaging system

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Actin Beta Antibody | 12141 gallery image 4

Staining of HeLa lysate with Human anti actin beta HRP (HCA147P)

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Actin Beta Antibody | 12141 gallery image 5

Sandwich ELISA analysis of human actin beta binding using Human anti Human actin beta antibody, clone 12141 (HCA147) as a capture reagent and biotinylated Mouse anti Human actin, clone 4C2 (MCA5775GA) as a detection reagent with purified human actin beta as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Asthmatic sufferer serum samples (orange and blue) and SLE serum sample (green) are shown. Beta actin was undetectable in normal serum samples

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  • Human anti Human Actin Beta
  • Human anti Human Actin Beta:DyLight®800
  • Human anti Human Actin Beta:DyLight®750
  • Human anti Human Actin Beta:DyLight®680
  • Human anti Human Actin Beta:HRP
  • Human anti Human Actin Beta
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    12141
  • Isotype
    HuCAL Fab bivalent
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA147WBdatasheet pdfdatasheet pdf0.1 mg
    HCA147
    HCA147D800WBdatasheet pdfdatasheet pdf0.1 mg
    HCA147D800
    HCA147D750WBdatasheet pdfdatasheet pdf0.1 mg
    HCA147D750
    HCA147D680WBdatasheet pdfdatasheet pdf0.1 mg
    HCA147D680
    HCA147PWBdatasheet pdfdatasheet pdf0.1 mg
    HCA147P
    HCA147GWBdatasheet pdfdatasheet pdf0.5 mg
    HCA147G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti human actin beta antibody, clone 12141 recognizes the 42kDa product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic 1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modelling.

      Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between the human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products.

      Actin beta is a commonly used Western blotting loading control for cell and tissue lysates across many species due to its ubiquitous expression and evolutionary conservation rendering antibodies against this target cross reactive with protein preparations from many different organisms.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      VertebratesExpected from Sequence
      InvertebratesExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to DyLight 800 - liquid.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to DyLight 750 - liquid.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to DyLight 680 - liquid.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to Horseradish Peroxidase (HRP) - liquid
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
    • Reconstitution
      Pack Size: 0.5 mgReconstitute with 5.0ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 0.5 mgReconstitute with 5.0ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
    • Preparation
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Purified Human beta-Actin
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml
      1.0 mg/ml
      1.0 mg/ml
      Total protein concentration 1.0 mg/ml
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 0.5 mgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.5 mgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of reconstitution.
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.

      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.

      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.

      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1001/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/10001/5000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/10001/10000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1001/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      HCA147 detects a band of approximately 42kDa in HeLa cell lysates.

      HCA147 is suitable for use as a loading control.
    • Western Blotting
      HCA147D800 is suitable for use as a loading control.
    • Western Blotting
      HCA147D750 is suitable for use as a loading control.
    • Western Blotting
      HCA147D680 is suitable for use as a loading control.
    • Western Blotting
      HCA147P is suitable for use as a loading control.
    • Western Blotting
      HCA147G detects a band of approximately 42kDa in HeLa cell lysates.

      HCA147G is suitable for use as a loading control.
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Actin Beta Antibody Formats

    Formats Clone Applications Sizes available
    Actin Beta Antibody : Purified 12141 WB 0.1 mg | 0.5 mg
    Actin Beta Antibody : HRP 12141 WB 0.1 mg
    Actin Beta Antibody : DyLight®800 12141 WB 0.1 mg
    Actin Beta Antibody : DyLight®680 12141 WB 0.1 mg
    Actin Beta Antibody : DyLight®750 12141 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
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      MCA2200PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      MCA2200B
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      MCA2200F
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
      MCA2489P
      Mouse anti c-Myc:RPEMCA2200PE100 TestsWB
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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          HuCAL Fab-dHLX-MSx2 Negative ControlHCA1020.1 mgC, E, F, P, WB
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          Recommended Positive Controls

            Histology Controls

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