IL-4 antibody | 13G7
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Mouse anti Horse Interleukin-4
- Product Type
- Monoclonal Antibody
- Clone
- 13G7
- Isotype
- IgG1
- Specificity
- IL-4
Antibody Quality and Performance Guarantee
| Mouse anti Horse interleukin-4 antibody, clone 13G7 recognizes horse interleukin-4 (IL-4). Interleukin-4 is a cytokine derived from Th2 cells (Mosmann and Coffman, 1989). It has a number of regulatory effects on B-cells, T-cells, monocytes and dendritic cells, as well as some non-immune cells such as endothelial cells and fibroblasts (Chomarat and Banchereau, 1998; Galli et al. 2005). IL-4 is the key cytokine for regulating immunoglobulin class switching to IgG1 and IgE ( Siebenkotten et al. 1992). Interleukin-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes (Miyajima et al. 1991, Hudak et al. 1987). Mouse anti Horse interleukin-4 antibody, clone 13G7, is able to detect equine IL-4 in an ELISA on supernatants from horse PBMC stimulated with PHA, PWM and ConA, but not from LPS stimulation (Wagner et al. 2006). |
- Target Species
- Horse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant equine IL-4
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X3.Ag8-653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Antibody Characterization Reference
-
Wagner, B. et al. (2006) A monoclonal antibody to equine interleukin-4.
Vet Immunol Immunopathol. 110 (3-4): 363-7.
- UniProt
- P42202
- Entrez Gene
- IL4
- GO Terms
- GO:0005125 cytokine activity
- GO:0005136 interleukin-4 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0042113 B cell activation
MCA6392GA
162255
163630
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