IL-17A antibody | 80-1
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Mouse anti Horse Interleukin-17A
- Product Type
- Monoclonal Antibody
- Clone
- 80-1
- Isotype
- IgG1
- Specificity
- IL-17A
| Mouse anti Horse interleukin-17A antibody, clone 80-1 recognizes horse interleukin-17A (IL-17A). The IL-17 family consists of six proteins (IL-17A to IL-17F) which function as pro-inflammatory cytokines, important in the defence against pathogens (Gurczynski and Moore, 2018). It is also considered a target for several autoimmune and inflammatory diseases. The predominant sources of IL-17A are lymphocytes, including CD4+, CD8+, gamma-delta T, invariant NKT, and innate lymphoid cells (ILCs) (Cua and Tato, 2010). However, the closest association is to Th17 cells which are generated from naïve CD4+ T cells (Park et al, 2005). Perkins et al. found that equine colostral immune cells, compared to PBMC, have an overall inflammatory phenotype shown by high IFN-gamma and IL-17 secretion (Perkins et al, 2014). The purified Mouse anti Horse interleukin-17A antibody, clone 80-1 (MCA6395GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Horse interleukin-17A antibody, clone 79-3 (MCA6396B) as the detection antibody. |
- Target Species
- Horse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant equine IL17A/IL-4 fusion protein
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X3.Ag8-653 myeloma cell line
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Detection Antibody:
MCA6396B
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for IL-17A antibody
-
Perkins, G.A. et al. (2014) Maternal T-lymphocytes in equine colostrum express a primarily inflammatory phenotype.
Vet Immunol Immunopathol. 161 (3-4): 141-50.
- UniProt
- A0A3Q2HZ94
MCA6395GA
162592
165475
166247
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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