IgGa antibody | CVS48
Mouse anti Horse IgGa antibody, clone CVS48 (MCA1902GA) used for the evaluation of equine IgG1 serum levels by ELISA.
Image caption:
Vaccination significantly increased serum titers of IgA, IgG1, and IgG4/7.
Optical density ratios from serum samples relative to a positive control of IgA (panel A), IgG1 (panel B), and IgG4/7 (panel C) antibodies among IM-only vaccinates (Group = Vx; n = 20) and controls (Group = C; n = 10). The bottoms and tops of the boxes represent the 25th and 75th percentiles, and the horizontal lines in the boxes represent the medians; the thin vertical lines extend to a multiple of 1.75 of the respective interquartile distance, and the small circles represent outliers. Asterisks indicate significant (P <0.05) difference from baseline (Week 0) among vaccinates. For IgA (Panel A), concentrations were significantly higher than baseline through week 11 after initial vaccination (12 weeks after booster injection); for controls, there were no significant differences in titers at any of the time point comparisons. For IgG1 (Panel B), concentrations were significantly higher than week 0 from weeks 3 through 11, and again at week 27. For controls, values at week 27 were significantly (P <0.05) higher than for all other times, but no other differences were significant. We attributed the higher titers at week 27 in both groups to be attributable to an artifact of testing or acquisition of natural antibody to PNAG. For IgG4/7 (Panel C), concentrations were significantly higher than week 0 from weeks 3 through 15; for controls, there were no significant differences among times.
From: Cohen ND, Cywes-Bentley C, Kahn SM, Bordin AI, Bray JM, Wehmeyer SG, et al. (2020)
Vaccination of yearling horses against poly-N-acetyl glucosamine fails to protect against infection with Streptococcus equi subspecies equi.
PLoS ONE 15(10): e0240479.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Horse IgGa antibody, clone CVS48 recognizes equine IgGa, also known as IgG1 (Lewis et al. 2008) and shows no significant binding to other IgG sub-classes.
In addition to Mouse anti Horse IgGa antibody, clone CVS48, we also offer a range of other monoclonal and polyclonal antibodies that are specific to equine immunoglobulins.
In addition to Mouse anti Horse IgGa antibody, clone CVS48, we also offer a range of other monoclonal and polyclonal antibodies that are specific to equine immunoglobulins.
Product Details
- Target Species
- Horse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified equine IgGa.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse X63-Ag8.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgGa antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This product is suitable for use in indirect ELISA applications.
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK003P | CJ | 1 Conjugation For 4mg Antibody | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK004P | CJ | 5 Conjugations For 4mg Antibody | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK005P | CJ | 1 Conjugation For 20mg Antibody | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK006P | CJ | 3 Conjugations For 40µg Antibody |
Product Specific References
References for IgGa antibody
-
Sheoran, A. S. et al. (1998) Monoclonal antibodies to subclass-specific antigenic determinants on equine immunoglobulin gamma chains and their characterization.
Vet. Immunol. Immunopathol. 62: 153-165. -
Lewis, M. J. et al. (2008) The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies.
Mol. Immunol. 45: 818-827. -
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62: 101-143 -
Cohen, N.D. et al. (2020) Vaccination of yearling horses against poly-N-acetyl glucosamine fails to protect against infection with Streptococcus equi subspecies equi.
PLoS One. 15 (10): e0240479.
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