HRP Conjugation Kit

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LYNX Rapid HRP Antibody Conjugation Kit

Product Type
Conjugation Kit
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
LNK001P CJ 1 Conjugation For 400µg Antibody
LNK002P CJ 3 Conjugations For 400µg Antibody
LNK003P CJ 1 Conjugation For 4mg Antibody
LNK004P CJ 5 Conjugations For 4mg Antibody
LNK005P CJ 1 Conjugation For 20mg Antibody
LNK006P CJ 3 Conjugations For 40µg Antibody
LYNX Rapid HRP Antibody Conjugation Kit® enables the rapid conjugation of a pre-prepared lyophilized mixture containing Horseradish peroxidase (HRP) label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in directional covalent bonding of HRP to the antibody.

The LYNX Rapid Conjugation kit® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.

Product Details

Reagents in the Kit
Pack Size: 1 Conjugation For 400µg Antibody
1 Vial of 100μg LYNX lyophilized HRP mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
Pack Size: 3 Conjugations For 400µg Antibody
3 Vials of 100ug LYNX lyophilized HRP mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
Pack Size: 1 Conjugation For 4mg Antibody
1 Vial of 1mg LYNX lyophilized HRP mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
Pack Size: 5 Conjugations For 4mg Antibody
5 Vials of 1mg LYNX lyophilized HRP mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
Pack Size: 1 Conjugation For 20mg Antibody
1 Vial of 5mg LYNX lyophilized HRP mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
Pack Size: 3 Conjugations For 40µg Antibody
3 Vials of 10μg LYNX lyophilized HRP mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
Preparing The Antibody
Pack Size: 1 Conjugation For 400µg Antibody
The following buffer solutions are recommended for preparing the antibody:

10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.

The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 100μg HRP you need to add between 100-400μg of antibody. For optimal results the antibody volume should be up to 100μl, at a concentration range of 0.5-5.0mg/ml.
Pack Size: 3 Conjugations For 400µg Antibody
The following buffer solutions are recommended for preparing the antibody:

10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.

The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 100μg HRP you need to add between 100-400μg of antibody. For optimal results the antibody volume should be up to 100μl, at a concentration range of 0.5-5.0mg/ml.
Pack Size: 1 Conjugation For 4mg Antibody
The following buffer solutions are recommended for preparing the antibody:

10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.

The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 1mg HRP you need to add between 1-4mg of antibody.. For optimal results the antibody volume should be up to 1ml, at a concentration range of 0.5-5.0mg/ml.
Pack Size: 5 Conjugations For 4mg Antibody
The following buffer solutions are recommended for preparing the antibody:

10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.

The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 4mg HRP you need to add between 1-4mg of antibody. For optimal results the antibody volume should be up to 1ml, at a concentration range of 0.5-5.0mg/ml.
Pack Size: 1 Conjugation For 20mg Antibody
The following buffer solutions are recommended for preparing the antibody:

10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.

The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 5mg HRP you need to add between 5-20mg of antibody. For optimal results the antibody volume should be up to 5ml, at a concentration range of 0.5-5.0mg/ml.
Pack Size: 3 Conjugations For 40µg Antibody
The following buffer solutions are recommended for preparing the antibody:

10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.

The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 10μg HRP you need to add between 10-40μg of antibody. For optimal results the antibody volume should be up to 10μl, at a concentration range of 0.5-5.0mg/ml.

Storage Information

Storage
Store kit at -20oC only.
Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended, although sodium azide should be avoided.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing.
Shelf Life
12 months after despatch

More Information

Licensed Use
These products and the methodology of conjugation are patent protected under United Kingdom patent number 2446088 and associated international patent applications. The purchase of this product conveys to the buyer the limited, non exclusive non-transferable right (without the right to resell repackage or further sublicense) under these patents to use the product to make conjugates for research and development purposes only. The purchaser cannot sell or otherwise transfer this product, or its components, or materials or data made using this product, or its components to a third party. Further information on purchasing licenses for diagnostic and other uses may be obtained by contacting Bio-Rad, at. Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE UNITED KINGDOM. Tel: +44 1865 852 700. E-mail: antibodies@bio-rad.com
Regulatory
For research purposes only

Applications of HRP Conjugation Kit

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Conjugation

We recommend that for each conjugation the user determines the best antibody:conjugate ratio.
Instructions For Use
1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.

2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

3. Replace cap onto vial and incubate at room temperature (20-25°C) for 3 hours, or overnight if preferred.

4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.

Product Specific References

References for HRP Conjugation Kit

  1. Bondzio, A. et al. (2011) Identification of differentially expressed proteins in ruminal epithelium in response to a concentrate-supplemented diet.
    Am J Physiol Gastrointest Liver Physiol. 301 (2): G260-8.
  2. Lichtmannegger, J. et al. (2016) Methanobactin reverses acute liver failure in a rat model of Wilson disease.
    J Clin Invest. 126 (7): 2721-35.