CD25 antibody | 15-1
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Mouse anti Horse CD25
- Product Type
- Monoclonal Antibody
- Clone
- 15-1
- Isotype
- IgG1
- Specificity
- CD25
Antibody Quality and Performance Guarantee
| Mouse anti Horse CD25, clone 15-1 recognizes horse CD25, also known as the interleukin-2 (IL-2) receptor subunit alpha. CD25 is expressed by activated T and B cells (Jackson et al. 1990, Butcher et al. 1990). CD25 expression on CD4+ T cells is used to identify regulatory T (Treg) cells. CD25 acts as the low affinity receptor for IL-2, but it also assembles into a heterotrimer with CD122 and CD132 to form the high affinity receptor. |
- Target Species
- Horse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant equine CD25
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X3.Ag8-653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/50 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
References for CD25 antibody
-
Wagner, B. et al. (2024) Monoclonal antibodies for equine CD25 improve detection of regulatory T cells in horses.
Vet Immunol Immunopathol. 274: 110790.
- UniProt
- A8VUE0
MCA6398GA
162382
163299
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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