CD11a/CD18 antibody | CVS9
Mouse anti Horse CD11a/CD18
- Product Type
- Monoclonal Antibody
- Clone
- CVS9
- Isotype
- IgG1
- Specificity
- CD11a/CD18
Antibody Quality and Performance Guarantee
| Mouse anti Horse CD11a/CD18 antibody, clone CVS9 recognizes the equine homolog of the human CD11a/CD18 cell surface antigen, a hetrodimer expressed on all equine cells of haemopoietic origin. Equine CD11a/CD18 has higher expression on some subpopulations of T-cells. In addition to the CVS9 clone, other CVS clones recognising equine MHC and cell surface antigens are available from Bio-Rad. |
- Target Species
- Horse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Equine leucocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD11a/CD18 antibody
-
Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991.
Vet Immunol Immunopathol. 42 (1): 3-60. -
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62 (2): 101-43. -
Hammond, S.A. et al. (1999) Functional characterization of equine dendritic cells propagated ex vivo using recombinant human GM-CSF and recombinant equine IL-4.
Vet Immunol Immunopathol. 71 (3-4): 197-214. -
McClure JT et al. (2001) Immunophenotypic classification of leukemia in 3 horses.
J Vet Intern Med. 15 (2): 144-52. -
Laval, K. et al. (2015) Equine Herpesvirus Type 1 Enhances Viral Replication in CD172a+ Monocytic Cells upon Adhesion to Endothelial Cells.
J Virol. 89 (21): 10912-23. -
Schröck, C. et al. (2017) Bone marrow-derived multipotent mesenchymal stromal cells from horses after euthanasia.
Vet Med Sci. 3 (4): 239-251. -
Theuerkauf, K. et al. (2022) Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome.
J Vet Diagn Invest. 10406387221077969.
MCA1081GA
0112
164687
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