CD11a/CD18 antibody | CVS9

Mouse anti Horse CD11a/CD18

Product Type
Monoclonal Antibody
Clone
CVS9
Isotype
IgG1
Specificity
CD11a/CD18

Product Code Applications Pack Size List Price Your Price Qty
MCA1081GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Horse CD11a/CD18 antibody, clone CVS9 recognizes the equine homolog of the human CD11a/CD18 cell surface antigen, a hetrodimer expressed on all equine cells of haemopoietic origin. Equine CD11a/CD18 has higher expression on some subpopulations of T-cells.

In addition to the CVS9 clone, other CVS clones recognising equine MHC and cell surface antigens are available from Bio-Rad.

Target Species
Horse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Equine leucocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD11a/CD18 antibody

  1. Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991.
    Vet Immunol Immunopathol. 42 (1): 3-60.
  2. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
    Vet Immunol Immunopathol. 62 (2): 101-43.
  3. McClure JT et al. (2001) Immunophenotypic classification of leukemia in 3 horses.
    J Vet Intern Med. 15 (2): 144-52.
  4. Hammond, S.A. et al. (1999) Functional characterization of equine dendritic cells propagated ex vivo using recombinant human GM-CSF and recombinant equine IL-4.
    Vet Immunol Immunopathol. 71 (3-4): 197-214.
  5. Laval, K. et al. (2015) Equine Herpesvirus Type 1 Enhances Viral Replication in CD172a+ Monocytic Cells upon Adhesion to Endothelial Cells.
    J Virol. 89 (21): 10912-23.
  6. Schröck, C. et al. (2017) Bone marrow-derived multipotent mesenchymal stromal cells from horses after euthanasia.
    Vet Med Sci. 3 (4): 239-251.
  7. Theuerkauf, K. et al. (2022) Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome.
    J Vet Diagn Invest. 10406387221077969.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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