IgE antibody | E6-2A1
Mouse anti Dog IgE antibody, clone E6-2A1 (MCA1895) used for the evaluation of mite specific IgE levels in canine samples by ELISA.
Image caption:
Median and interquartile range of DF- and DP-specific IgE antibodies. Aged healthy dogs (> 3 years) had high levels of all antibody types to both species of HDM. In cases of CAD (+IDT or -IDT to DF/DP) high IgE levels were in accordance with IDT results. Dotted line; cut-off levels for each ELISA.
From: Khantavee N, Chanthick C, Tungtrongchitr A, Techakriengkrai N, Suradhat S, Sookrung N, Roytrakul S, Prapasarakul N.
Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus in atopic dogs.
BMC Vet Res. 2021 Feb 5;17(1):71.
doi: 10.1186/s12917-021-02768-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Dog IgE
- Product Type
- Monoclonal Antibody
- Clone
- E6-2A1
- Isotype
- IgG2a
- Specificity
- IgE
Antibody Quality and Performance Guarantee
| Mouse anti Dog IgE antibody, clone E6-2A1 recognises canine IgE. E6-2A1 does not cross react with dog IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-2A1 demonstrates a single major band of ~62-65 kDa under reducing conditions. |
- Target Species
- Dog
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Immunogen
- Affinity purified IgE preparation from heavily parasitized and allergic dog serum.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Mouse anti Dog IgE antibody, clone E6-2A1 may be used as a capture antibody in a sandwich ELISA in combination with MCA1896B as the detection antibody.
References for IgE antibody
-
Lin, T.Y. et al. (2006) Generation and characterization of bone marrow-derived cultured canine mast cells.
Vet Immunol Immunopathol. 113 (1-2): 37-52. -
Lin, T.Y. & London, C.A. (2006) A functional comparison of canine and murine bone marrow derived cultured mast cells.
Vet Immunol Immunopathol. 114 (3-4): 320-34. -
Khantavee, N. et al. (2020) Antibody levels to Malassezia pachydermatis. and Staphylococcus pseudintermedius. in atopic dogs and their relationship with lesion scores.
Vet Dermatol. 31 (2): 111-115. -
Khantavee, N. et al. (2021) Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae. and Dermatophagoides pteronyssinus. in atopic dogs.
BMC Vet Res. 17 (1): 71. -
Mason, K. et al. (2023) Immunoglobulin E-specific allergens against leaf in serum of dogs with clinical features of grass leaf allergy.
Vet Dermatol. 34 (5): 393-403.
- RRID
- AB_323084
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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