IgE antibody | E6-2A1
Mouse anti Dog IgE antibody, clone E6-2A1 (MCA1895) used for the evaluation of mite specific IgE levels in canine samples by ELISA.
Image caption:
Median and interquartile range of DF- and DP-specific IgE antibodies. Aged healthy dogs (> 3 years) had high levels of all antibody types to both species of HDM. In cases of CAD (+IDT or -IDT to DF/DP) high IgE levels were in accordance with IDT results. Dotted line; cut-off levels for each ELISA.
From: Khantavee N, Chanthick C, Tungtrongchitr A, Techakriengkrai N, Suradhat S, Sookrung N, Roytrakul S, Prapasarakul N.
Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus in atopic dogs.
BMC Vet Res. 2021 Feb 5;17(1):71.
doi: 10.1186/s12917-021-02768-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Dog IgE antibody, clone E6-2A1 recognises canine IgE. E6-2A1 does not cross react with dog IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-2A1 demonstrates a single major band of ~62-65 kDa under reducing conditions.
Product Details
- Target Species
- Dog
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
- Immunogen
- Affinity purified IgE preparation from heavily parasitized and allergic dog serum.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgE antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- The antibody may be used as a capture antibody in a sandwich ELISA in combination with MCA1896B as the detection antibody.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for IgE antibody
-
Lin, T.Y. et al. (2006) Generation and characterization of bone marrow-derived cultured canine mast cells.
Vet Immunol Immunopathol. 113 (1-2): 37-52. -
Lin, T.Y. & London, C.A. (2006) A functional comparison of canine and murine bone marrow derived cultured mast cells.
Vet Immunol Immunopathol. 114 (3-4): 320-34. -
Khantavee, N. et al. (2020) Antibody levels to Malassezia pachydermatis. and Staphylococcus pseudintermedius. in atopic dogs and their relationship with lesion scores.
Vet Dermatol. 31 (2): 111-115. -
Khantavee, N. et al. (2021) Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae. and Dermatophagoides pteronyssinus. in atopic dogs.
BMC Vet Res. 17 (1): 71.
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