WC1 antibody | CC101
|Mouse anti Bovine WC1 antibody, clone CC101, recognizes a subset of WC1+ T-cells expressing the WC1.1 isoform (
The bovine WC1 cell surface antigen is expressed by a population of gamma/delta T-cells that lack CD2, CD4 and CD8, but express CD3. WC1 expression appears to be heterogeneous and antibodies to this cluster show differing reaction patterns (Crocker et al. 1993).
Mouse anti bovine WC1, clone CC101, immunoprecipitates a 215 kDa molecule from bovine cells and also recognizes the swine homolog of WC1, which is a 180 kDa molecule. In pigs, the 180 kDa molecule is expressed by a gamma/delta TCR positive T-cell population that also lack CD2, CD4 and CD8 (Carr et al. 1994).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Pig Sheep
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Con A stimulated bovine lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use10ul of the suggested working dilution to label 106 cells in 100ul.
References for WC1 antibody
Carr, M.M. et al. (1994) Expression on porcine gamma/delta lymphocytes of a phylogenetically conserved surface antigen previously restricted in expression to ruminant gamma/delta T lymphocytes.
Immunology 81: 36-40.
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle.
Vet Immunol Immunopathol. 39: 25-48.
MacHugh, N. et al. (1993) Clustering of monoclonal antibodies recognizing different members of the WC1 gene family.
Vet Immunol Immunopathol. 39: 155-60.
Crocker, G. et al. (1993) Analysis of the gamma/delta T cell restricted antigen WC1.
Vet Immunol Immunopathol. 39: 137-44.
Lund, B. et al. (1993) Expression of T19 (WC1) molecules by ovine lymphocytes.
Vet Immunol Immunopathol. 39: 145-53.
Schröder, A.C. & Hamann, J. (2005) The influence of technical factors on differential cell count in milk.
J Dairy Res. 72: 153-8.
Liu, X. et al. (2014) Crusted scabies is associated with increased IL-17 secretion by skin T cells.
Parasite Immunol. 36: 594-604.
Patarroyo, J.H. et al. (2009) Immune response of bovines stimulated by synthetic vaccine SBm7462 against Rhipicephalus (Boophilus) microplus.
Vet Parasitol. 166: 333-9.
View The Latest Product References
Heiser, A. et al. (2015) Grazing dairy cows had decreased interferon-γ, tumor necrosis factor, and interleukin-17, and increased expression of interleukin-10 during the first week after calving.
J Dairy Sci. 98: 937-46.
Takamatsu, H.H. et al. (2006) Porcine gammadelta T cells: possible roles on the innate and adaptive immune responses following virus infection.
Vet Immunol Immunopathol. 112: 49-61.
Sedlak, C. et al. (2014) IL-12 and IL-18 induce interferon-γ production and de novo CD2 expression in porcine γδ T cells.
Dev Comp Immunol. 47: 115-22.
Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
Sci Rep. 7 (1): 3441.
Hussen, J. et al. (2018) Expression Patterns of Cell Adhesion Molecules on CD4+ T Cells and WC1+ T Cells in the Peripheral Blood of Dromedary Camels.
Pakistan Veterinary Journal. 38 (03): 231-236.
Al-Ashqar, R.A. et al. (2015) The CD markers of camel (Camelus dromedarius.) milk cells during mastitis: the LPAM-1 expression is an indication of possible mucosal nature of the cellular trafficking.
Res Vet Sci. 99: 77-81.
Al-Mohammed Salem Kazem, T. et al. (2012) The Cellular Populations of Normal Camel (<i>Camelus dromedaries</i>) Milk
Open Journal of Veterinary Medicine. 02 (04): 262-5.
Kato-Mori, Y. et al. (2021) Characterization of a variant CD4 molecule in Japanese Black cattle.
Vet Immunol Immunopathol. 232: 110167.
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