WC1 antibody | CC101

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Mouse anti Bovine WC1:FITC

Mouse anti Bovine WC1

Product Type
Monoclonal Antibody
Clone
CC101
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1655F F 0.1 mg
MCA1655GA C F IP 0.1 mg
MCA1655G C IP 0.25 mg
Mouse anti Bovine WC1 antibody, clone CC101, recognizes a subset of WC1+ T-cells expressing the WC1.1 isoform (MacHugh et al. 1993). The bovine WC1 cell surface antigen is expressed by a population of gamma/delta T-cells that lack CD2, CD4 and CD8, but express CD3. WC1 expression appears to be heterogeneous and antibodies to this cluster show differing reaction patterns (Crocker et al. 1993).

Mouse anti bovine WC1, clone CC101, immunoprecipitates a 215 kDa molecule from bovine cells and also recognizes the swine homolog of WC1, which is a 180 kDa molecule. In pigs, the 180 kDa molecule is expressed by a gamma/delta TCR positive T-cell population that also lack CD2, CD4 and CD8 (Carr et al. 1994).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
Pack Size: 0.1 mg
0.09% Sodium Azide (NaN3)
Pack Size: 0.25 mg
0.09%Sodium Azide
Immunogen
Con A stimulated bovine lymphocytes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgG concentration 1.0 mg/ml
Pack Size: 0.25 mg
IgG concentration 1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P30205 Related reagents
Entrez Gene
CD163L1 Related reagents
GO Terms
GO:0016020 membrane
GO:0005576 extracellular region
GO:0005044 scavenger receptor activity
Regulatory
For research purposes only

Applications of WC1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F 100 Tests F
Mouse IgG2a Negative Control MCA929 100 Tests F

Product Specific References

References for WC1 antibody

  1. Carr, M.M. et al. (1994) Expression on porcine gamma/delta lymphocytes of a phylogenetically conserved surface antigen previously restricted in expression to ruminant gamma/delta T lymphocytes.
    Immunology 81: 36-40.
  2. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle.
    Vet Immunol Immunopathol. 39: 25-48.
  3. MacHugh, N. et al. (1993) Clustering of monoclonal antibodies recognizing different members of the WC1 gene family.
    Vet Immunol Immunopathol. 39: 155-60.
  4. Crocker, G. et al. (1993) Analysis of the gamma/delta T cell restricted antigen WC1.
    Vet Immunol Immunopathol. 39: 137-44.
  5. Lund, B. et al. (1993) Expression of T19 (WC1) molecules by ovine lymphocytes.
    Vet Immunol Immunopathol. 39: 145-53.
  6. Schröder, A.C. & Hamann, J. (2005) The influence of technical factors on differential cell count in milk.
    J Dairy Res. 72: 153-8.
  7. Liu, X. et al. (2014) Crusted scabies is associated with increased IL-17 secretion by skin T cells.
    Parasite Immunol. 36: 594-604.
  8. Patarroyo, J.H. et al. (2009) Immune response of bovines stimulated by synthetic vaccine SBm7462 against Rhipicephalus (Boophilus) microplus.
    Vet Parasitol. 166: 333-9.
  9. Heiser, A. et al. (2015) Grazing dairy cows had decreased interferon-γ, tumor necrosis factor, and interleukin-17, and increased expression of interleukin-10 during the first week after calving.
    J Dairy Sci. 98: 937-46.
  10. Takamatsu, H.H. et al. (2006) Porcine gammadelta T cells: possible roles on the innate and adaptive immune responses following virus infection.
    Vet Immunol Immunopathol. 112: 49-61.
  11. Sedlak, C. et al. (2014) IL-12 and IL-18 induce interferon-γ production and de novo CD2 expression in porcine γδ T cells.
    Dev Comp Immunol. 47: 115-22.
  12. Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
    Sci Rep. 7 (1): 3441.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.
  2. Wijngaard, P. et al. (1992) Molecular characterization of the WC1 antigen expressed specifically on bovine CD4-CD8- gamma delta T lymphocytes.
    J Immunol. 149: 3273-7.